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Distinct features of rabbit and human adipose-derived mesenchymal stem cells: implications for biotechnology and translational research

INTRODUCTION: Owing to their similarity with humans, rabbits are useful for multiple applications in biotechnology and translational research from basic to preclinical studies. In this sense, mesenchymal stem cells (MSCs) are known for their therapeutic potential and promising future in regenerative...

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Autores principales: Zomer, Helena Debiazi, Roballo, Kelly CS, Lessa, Thais Borges, Bressan, Fabiana Fernandes, Gonçalves, Natália Nardeli, Meirelles, Flávio Vieira, Trentin, Andrea Gonçalves, Ambrósio, Carlos Eduardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6204872/
https://www.ncbi.nlm.nih.gov/pubmed/30425533
http://dx.doi.org/10.2147/SCCAA.S175749
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author Zomer, Helena Debiazi
Roballo, Kelly CS
Lessa, Thais Borges
Bressan, Fabiana Fernandes
Gonçalves, Natália Nardeli
Meirelles, Flávio Vieira
Trentin, Andrea Gonçalves
Ambrósio, Carlos Eduardo
author_facet Zomer, Helena Debiazi
Roballo, Kelly CS
Lessa, Thais Borges
Bressan, Fabiana Fernandes
Gonçalves, Natália Nardeli
Meirelles, Flávio Vieira
Trentin, Andrea Gonçalves
Ambrósio, Carlos Eduardo
author_sort Zomer, Helena Debiazi
collection PubMed
description INTRODUCTION: Owing to their similarity with humans, rabbits are useful for multiple applications in biotechnology and translational research from basic to preclinical studies. In this sense, mesenchymal stem cells (MSCs) are known for their therapeutic potential and promising future in regenerative medicine. As many studies have been using rabbit adipose-derived MSCs (ASCs) as a model of human ASCs (hASCs), it is fundamental to compare their characteristics and understand how distinct features could affect the translation to human medicine. OBJECTIVE: The aim of this study was to comparatively characterize rabbit ASCs (rASCs) and hASCs to further uses in biotechnology and translational studies. MATERIALS AND METHODS: rASCs and hASCs were isolated and characterized by their immunophenotype, differentiation potential, proliferative profile, and nuclear stability in vitro. RESULTS AND DISCUSSION: Both ASCs presented differentiation potential to osteocytes, chondrocytes, and adipocytes and shared similar immunophenotype expression to CD105+, CD34−, and CD45−, but rabbit cells expressed significantly lower CD73 and CD90 than human cells. In addition, rASCs presented greater clonogenic potential and proliferation rate than hASCs but no difference in nuclear alterations. CONCLUSION: The distinct features of rASCs and hASCs can positively or negatively affect their use for different applications in biotechnology (such as cell reprogramming) and translational studies (such as cell transplantation, tissue engineering, and pharmacokinetics). Nevertheless, the particularities between rabbit and human MSCs should not prevent rabbit use in preclinical models, but care should be taken to interpret results and properly translate animal findings to medicine.
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spelling pubmed-62048722018-11-13 Distinct features of rabbit and human adipose-derived mesenchymal stem cells: implications for biotechnology and translational research Zomer, Helena Debiazi Roballo, Kelly CS Lessa, Thais Borges Bressan, Fabiana Fernandes Gonçalves, Natália Nardeli Meirelles, Flávio Vieira Trentin, Andrea Gonçalves Ambrósio, Carlos Eduardo Stem Cells Cloning Original Research INTRODUCTION: Owing to their similarity with humans, rabbits are useful for multiple applications in biotechnology and translational research from basic to preclinical studies. In this sense, mesenchymal stem cells (MSCs) are known for their therapeutic potential and promising future in regenerative medicine. As many studies have been using rabbit adipose-derived MSCs (ASCs) as a model of human ASCs (hASCs), it is fundamental to compare their characteristics and understand how distinct features could affect the translation to human medicine. OBJECTIVE: The aim of this study was to comparatively characterize rabbit ASCs (rASCs) and hASCs to further uses in biotechnology and translational studies. MATERIALS AND METHODS: rASCs and hASCs were isolated and characterized by their immunophenotype, differentiation potential, proliferative profile, and nuclear stability in vitro. RESULTS AND DISCUSSION: Both ASCs presented differentiation potential to osteocytes, chondrocytes, and adipocytes and shared similar immunophenotype expression to CD105+, CD34−, and CD45−, but rabbit cells expressed significantly lower CD73 and CD90 than human cells. In addition, rASCs presented greater clonogenic potential and proliferation rate than hASCs but no difference in nuclear alterations. CONCLUSION: The distinct features of rASCs and hASCs can positively or negatively affect their use for different applications in biotechnology (such as cell reprogramming) and translational studies (such as cell transplantation, tissue engineering, and pharmacokinetics). Nevertheless, the particularities between rabbit and human MSCs should not prevent rabbit use in preclinical models, but care should be taken to interpret results and properly translate animal findings to medicine. Dove Medical Press 2018-10-23 /pmc/articles/PMC6204872/ /pubmed/30425533 http://dx.doi.org/10.2147/SCCAA.S175749 Text en © 2018 Zomer et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Zomer, Helena Debiazi
Roballo, Kelly CS
Lessa, Thais Borges
Bressan, Fabiana Fernandes
Gonçalves, Natália Nardeli
Meirelles, Flávio Vieira
Trentin, Andrea Gonçalves
Ambrósio, Carlos Eduardo
Distinct features of rabbit and human adipose-derived mesenchymal stem cells: implications for biotechnology and translational research
title Distinct features of rabbit and human adipose-derived mesenchymal stem cells: implications for biotechnology and translational research
title_full Distinct features of rabbit and human adipose-derived mesenchymal stem cells: implications for biotechnology and translational research
title_fullStr Distinct features of rabbit and human adipose-derived mesenchymal stem cells: implications for biotechnology and translational research
title_full_unstemmed Distinct features of rabbit and human adipose-derived mesenchymal stem cells: implications for biotechnology and translational research
title_short Distinct features of rabbit and human adipose-derived mesenchymal stem cells: implications for biotechnology and translational research
title_sort distinct features of rabbit and human adipose-derived mesenchymal stem cells: implications for biotechnology and translational research
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6204872/
https://www.ncbi.nlm.nih.gov/pubmed/30425533
http://dx.doi.org/10.2147/SCCAA.S175749
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