The therapeutic effect in gliomas of nanobubbles carrying siRNA combined with ultrasound-targeted destruction

BACKGROUND: Nanobubbles (NBs) combined with ultrasound-targeted destruction (UTD) have become promising potential carriers for drug or siRNA delivery. Due to their nano-size, NBs could penetrate tumor blood vessels and accumulate in intercellular spaces so that “sonoporation” induced by UTD would ac...

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Autores principales: Cai, Wenbin, Lv, Wei, Feng, Yang, Yang, Hengli, Zhang, Yajun, Yang, Guodong, Duan, Yunyou, Wang, Jia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2018
Materias:
Original Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6205539/
https://www.ncbi.nlm.nih.gov/pubmed/30425489
http://dx.doi.org/10.2147/IJN.S164760
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author Cai, Wenbin
Lv, Wei
Feng, Yang
Yang, Hengli
Zhang, Yajun
Yang, Guodong
Duan, Yunyou
Wang, Jia
author_facet Cai, Wenbin
Lv, Wei
Feng, Yang
Yang, Hengli
Zhang, Yajun
Yang, Guodong
Duan, Yunyou
Wang, Jia
author_sort Cai, Wenbin
collection PubMed
description BACKGROUND: Nanobubbles (NBs) combined with ultrasound-targeted destruction (UTD) have become promising potential carriers for drug or siRNA delivery. Due to their nano-size, NBs could penetrate tumor blood vessels and accumulate in intercellular spaces so that “sonoporation” induced by UTD would act directly on the tumor cells to increase cell membrane permeability. METHODS: Based on the successful the fabrication of NBs, we synthesized NBs carrying siRNA (NBs-siRNA) by using a biotin-streptavidin system. We then utilized ultrasound irradiation (UI)-targeted NBs-siRNA to improve siRNA transfection and achieve the inhibition of glioma growth. RESULTS: NBs as carriers combined with UI effectively enhanced siRNA transfection and the effect of silencing targeted genes in vitro. Additionally, a better therapeutic effect was shown in the NBs-siRNA with UI group in vivo compared with that of microbubbles (MBs) with UI or NBs-siRNA without UI. CONCLUSION: These results indicated that NBs combined with UTD might be an ideal delivery vector for siRNA to achieve the noninvasive treatment of glioma.
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spelling pubmed-62055392018-11-13 The therapeutic effect in gliomas of nanobubbles carrying siRNA combined with ultrasound-targeted destruction Cai, Wenbin Lv, Wei Feng, Yang Yang, Hengli Zhang, Yajun Yang, Guodong Duan, Yunyou Wang, Jia Int J Nanomedicine Original Research BACKGROUND: Nanobubbles (NBs) combined with ultrasound-targeted destruction (UTD) have become promising potential carriers for drug or siRNA delivery. Due to their nano-size, NBs could penetrate tumor blood vessels and accumulate in intercellular spaces so that “sonoporation” induced by UTD would act directly on the tumor cells to increase cell membrane permeability. METHODS: Based on the successful the fabrication of NBs, we synthesized NBs carrying siRNA (NBs-siRNA) by using a biotin-streptavidin system. We then utilized ultrasound irradiation (UI)-targeted NBs-siRNA to improve siRNA transfection and achieve the inhibition of glioma growth. RESULTS: NBs as carriers combined with UI effectively enhanced siRNA transfection and the effect of silencing targeted genes in vitro. Additionally, a better therapeutic effect was shown in the NBs-siRNA with UI group in vivo compared with that of microbubbles (MBs) with UI or NBs-siRNA without UI. CONCLUSION: These results indicated that NBs combined with UTD might be an ideal delivery vector for siRNA to achieve the noninvasive treatment of glioma. Dove Medical Press 2018-10-24 /pmc/articles/PMC6205539/ /pubmed/30425489 http://dx.doi.org/10.2147/IJN.S164760 Text en © 2018 Cai et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Cai, Wenbin
Lv, Wei
Feng, Yang
Yang, Hengli
Zhang, Yajun
Yang, Guodong
Duan, Yunyou
Wang, Jia
The therapeutic effect in gliomas of nanobubbles carrying siRNA combined with ultrasound-targeted destruction
title The therapeutic effect in gliomas of nanobubbles carrying siRNA combined with ultrasound-targeted destruction
title_full The therapeutic effect in gliomas of nanobubbles carrying siRNA combined with ultrasound-targeted destruction
title_fullStr The therapeutic effect in gliomas of nanobubbles carrying siRNA combined with ultrasound-targeted destruction
title_full_unstemmed The therapeutic effect in gliomas of nanobubbles carrying siRNA combined with ultrasound-targeted destruction
title_short The therapeutic effect in gliomas of nanobubbles carrying siRNA combined with ultrasound-targeted destruction
title_sort therapeutic effect in gliomas of nanobubbles carrying sirna combined with ultrasound-targeted destruction
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6205539/
https://www.ncbi.nlm.nih.gov/pubmed/30425489
http://dx.doi.org/10.2147/IJN.S164760
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