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Chronic heart failure is characterized by altered mitochondrial function and structure in circulating leucocytes
Oxidative stress is currently viewed as a key factor in the genesis and progression of Heart Failure (HF). The aim of this study was to characterize the mitochondrial changes linked to oxidative stress generation in circulating peripheral blood mononuclear cells isolated from chronic HF patients (HF...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6205552/ https://www.ncbi.nlm.nih.gov/pubmed/30416677 http://dx.doi.org/10.18632/oncotarget.26164 |
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author | Coluccia, Roberta Raffa, Salvatore Ranieri, Danilo Micaloni, Andrea Valente, Sabatino Salerno, Gerardo Scrofani, Cristina Testa, Marco Gallo, Giovanna Pagannone, Erika Torrisi, Maria Rosaria Volpe, Massimo Rubattu, Speranza |
author_facet | Coluccia, Roberta Raffa, Salvatore Ranieri, Danilo Micaloni, Andrea Valente, Sabatino Salerno, Gerardo Scrofani, Cristina Testa, Marco Gallo, Giovanna Pagannone, Erika Torrisi, Maria Rosaria Volpe, Massimo Rubattu, Speranza |
author_sort | Coluccia, Roberta |
collection | PubMed |
description | Oxidative stress is currently viewed as a key factor in the genesis and progression of Heart Failure (HF). The aim of this study was to characterize the mitochondrial changes linked to oxidative stress generation in circulating peripheral blood mononuclear cells isolated from chronic HF patients (HF_PBMCs) in order to highlight the involvement of mitochondrial dysfunction in the pathophysiology of HF. To assess the production of reactive oxygen species (ROS), mitochondrial function and ultrastructure and the mitophagic flux in circulating PBMCs we enrolled 15 patients with HF and a control group of ten healthy subjects. The HF_PBMCs showed a mitochondrial population consisting of damaged and less functional organelles responsible of higher superoxide anion production both at baseline and under in vitro stress conditions, with evidence of cellular apoptosis. Although the mitophagic flux at baseline was enhanced in HF_PBMCs at level similar to those that could be achieved in control PBMCs only under inflammatory stress conditions, the activation of mitophagy was unable to preserve a proper mitochondrial dynamics upon stress stimuli in HF. In summary, circulating HF_PBMCs show structural and functional derangements of mitochondria with overproduction of reactive oxidant species. This mitochondrial failure sustains a leucocyte dysfunctional status in the blood that may contribute to development and persistence of stress conditions within the cardiovascular system in HF. |
format | Online Article Text |
id | pubmed-6205552 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-62055522018-11-09 Chronic heart failure is characterized by altered mitochondrial function and structure in circulating leucocytes Coluccia, Roberta Raffa, Salvatore Ranieri, Danilo Micaloni, Andrea Valente, Sabatino Salerno, Gerardo Scrofani, Cristina Testa, Marco Gallo, Giovanna Pagannone, Erika Torrisi, Maria Rosaria Volpe, Massimo Rubattu, Speranza Oncotarget Research Paper: Pathology Oxidative stress is currently viewed as a key factor in the genesis and progression of Heart Failure (HF). The aim of this study was to characterize the mitochondrial changes linked to oxidative stress generation in circulating peripheral blood mononuclear cells isolated from chronic HF patients (HF_PBMCs) in order to highlight the involvement of mitochondrial dysfunction in the pathophysiology of HF. To assess the production of reactive oxygen species (ROS), mitochondrial function and ultrastructure and the mitophagic flux in circulating PBMCs we enrolled 15 patients with HF and a control group of ten healthy subjects. The HF_PBMCs showed a mitochondrial population consisting of damaged and less functional organelles responsible of higher superoxide anion production both at baseline and under in vitro stress conditions, with evidence of cellular apoptosis. Although the mitophagic flux at baseline was enhanced in HF_PBMCs at level similar to those that could be achieved in control PBMCs only under inflammatory stress conditions, the activation of mitophagy was unable to preserve a proper mitochondrial dynamics upon stress stimuli in HF. In summary, circulating HF_PBMCs show structural and functional derangements of mitochondria with overproduction of reactive oxidant species. This mitochondrial failure sustains a leucocyte dysfunctional status in the blood that may contribute to development and persistence of stress conditions within the cardiovascular system in HF. Impact Journals LLC 2018-10-12 /pmc/articles/PMC6205552/ /pubmed/30416677 http://dx.doi.org/10.18632/oncotarget.26164 Text en Copyright: © 2018 Coluccia et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (http://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper: Pathology Coluccia, Roberta Raffa, Salvatore Ranieri, Danilo Micaloni, Andrea Valente, Sabatino Salerno, Gerardo Scrofani, Cristina Testa, Marco Gallo, Giovanna Pagannone, Erika Torrisi, Maria Rosaria Volpe, Massimo Rubattu, Speranza Chronic heart failure is characterized by altered mitochondrial function and structure in circulating leucocytes |
title | Chronic heart failure is characterized by altered mitochondrial function and structure in circulating leucocytes |
title_full | Chronic heart failure is characterized by altered mitochondrial function and structure in circulating leucocytes |
title_fullStr | Chronic heart failure is characterized by altered mitochondrial function and structure in circulating leucocytes |
title_full_unstemmed | Chronic heart failure is characterized by altered mitochondrial function and structure in circulating leucocytes |
title_short | Chronic heart failure is characterized by altered mitochondrial function and structure in circulating leucocytes |
title_sort | chronic heart failure is characterized by altered mitochondrial function and structure in circulating leucocytes |
topic | Research Paper: Pathology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6205552/ https://www.ncbi.nlm.nih.gov/pubmed/30416677 http://dx.doi.org/10.18632/oncotarget.26164 |
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