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MicroRNA expression profile during different conditions of hypoxia

INTRODUCTION: MicroRNAs (miRNAs) are small non coding RNAs which play a role in several cellular processes. MiRNA expression is influenced by oxidative stress, inflammatory cascade and hypoxia. Effects of different types of hypoxia (intermittent and chronic) have been poorly investigated. The aim of...

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Autores principales: Lacedonia, Donato, Scioscia, Giulia, Pia Palladino, Grazia, Gallo, Crescenzio, Carpagnano, Giovanna Elisiana, Sabato, Roberto, Foschino Barbaro, Maria Pia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6205556/
https://www.ncbi.nlm.nih.gov/pubmed/30416683
http://dx.doi.org/10.18632/oncotarget.26210
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author Lacedonia, Donato
Scioscia, Giulia
Pia Palladino, Grazia
Gallo, Crescenzio
Carpagnano, Giovanna Elisiana
Sabato, Roberto
Foschino Barbaro, Maria Pia
author_facet Lacedonia, Donato
Scioscia, Giulia
Pia Palladino, Grazia
Gallo, Crescenzio
Carpagnano, Giovanna Elisiana
Sabato, Roberto
Foschino Barbaro, Maria Pia
author_sort Lacedonia, Donato
collection PubMed
description INTRODUCTION: MicroRNAs (miRNAs) are small non coding RNAs which play a role in several cellular processes. MiRNA expression is influenced by oxidative stress, inflammatory cascade and hypoxia. Effects of different types of hypoxia (intermittent and chronic) have been poorly investigated. The aim of this study was to evaluate how intermittent and chronic hypoxia influence the expression of a pool of miRNAs. RESULTS: Subjects with HI presented higher levels of miR-21, miR-23b, miR-145 and miR-210 compared to the other groups, while higher levels of miR-26 was observed in the HC group. Subjects with HCHI had lower levels of all selected miRNAs. A strong correlation was found between miR-23b and miR-210 and both correlated with PaO2, age and FEV1. MiR-145 is correlated with miR-21 but no correlations were found with other parameters. The level of miR-26a seems to be correlated only with BMI. MATERIALS AND METHODS: We used RT-PCR to detect the miRNAs expression in three different models of hypoxemia: intermittent (HI), chronic (HC) and both of them (HCHI). Expression of miRNAs was analyzed using ANOVA and post hoc analysis, moreover, Spearman correlation and Cluster analysis were applied to study the relationship between miRNAs and main clinical parameters. CONCLUSIONS: Intermittent hypoxia induces the expression of some miRNAs more than chronic hypoxia. These miRNAs may play an important role in the development of different diseases usually associated with OSA such as cardiovascular disease. In addition, mechanisms involved in cancer progression may be induced in the presence of chronic and more often intermittent hypoxia.
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spelling pubmed-62055562018-11-09 MicroRNA expression profile during different conditions of hypoxia Lacedonia, Donato Scioscia, Giulia Pia Palladino, Grazia Gallo, Crescenzio Carpagnano, Giovanna Elisiana Sabato, Roberto Foschino Barbaro, Maria Pia Oncotarget Research Paper INTRODUCTION: MicroRNAs (miRNAs) are small non coding RNAs which play a role in several cellular processes. MiRNA expression is influenced by oxidative stress, inflammatory cascade and hypoxia. Effects of different types of hypoxia (intermittent and chronic) have been poorly investigated. The aim of this study was to evaluate how intermittent and chronic hypoxia influence the expression of a pool of miRNAs. RESULTS: Subjects with HI presented higher levels of miR-21, miR-23b, miR-145 and miR-210 compared to the other groups, while higher levels of miR-26 was observed in the HC group. Subjects with HCHI had lower levels of all selected miRNAs. A strong correlation was found between miR-23b and miR-210 and both correlated with PaO2, age and FEV1. MiR-145 is correlated with miR-21 but no correlations were found with other parameters. The level of miR-26a seems to be correlated only with BMI. MATERIALS AND METHODS: We used RT-PCR to detect the miRNAs expression in three different models of hypoxemia: intermittent (HI), chronic (HC) and both of them (HCHI). Expression of miRNAs was analyzed using ANOVA and post hoc analysis, moreover, Spearman correlation and Cluster analysis were applied to study the relationship between miRNAs and main clinical parameters. CONCLUSIONS: Intermittent hypoxia induces the expression of some miRNAs more than chronic hypoxia. These miRNAs may play an important role in the development of different diseases usually associated with OSA such as cardiovascular disease. In addition, mechanisms involved in cancer progression may be induced in the presence of chronic and more often intermittent hypoxia. Impact Journals LLC 2018-10-12 /pmc/articles/PMC6205556/ /pubmed/30416683 http://dx.doi.org/10.18632/oncotarget.26210 Text en Copyright: © 2018 Lacedonia et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (http://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Lacedonia, Donato
Scioscia, Giulia
Pia Palladino, Grazia
Gallo, Crescenzio
Carpagnano, Giovanna Elisiana
Sabato, Roberto
Foschino Barbaro, Maria Pia
MicroRNA expression profile during different conditions of hypoxia
title MicroRNA expression profile during different conditions of hypoxia
title_full MicroRNA expression profile during different conditions of hypoxia
title_fullStr MicroRNA expression profile during different conditions of hypoxia
title_full_unstemmed MicroRNA expression profile during different conditions of hypoxia
title_short MicroRNA expression profile during different conditions of hypoxia
title_sort microrna expression profile during different conditions of hypoxia
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6205556/
https://www.ncbi.nlm.nih.gov/pubmed/30416683
http://dx.doi.org/10.18632/oncotarget.26210
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