Cargando…
Direct observation of selective autophagy induction in cells and tissues by self-assembled chiral nanodevice
The interactions between chiral nanomaterials and organisms are still challenging and mysterious. Here, a chiral nanodevice made of yolk–shell nanoparticles tetrahedron (UYTe), centralized with upconversion nanoparticles (UCNPs), was fabricated to induce autophagy in vivo. The proposed chiral nanode...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6206072/ https://www.ncbi.nlm.nih.gov/pubmed/30374052 http://dx.doi.org/10.1038/s41467-018-06946-z |
_version_ | 1783366294761897984 |
---|---|
author | Sun, Maozhong Hao, Tiantian Li, Xiaoyun Qu, Aihua Xu, Liguang Hao, Changlong Xu, Chuanlai Kuang, Hua |
author_facet | Sun, Maozhong Hao, Tiantian Li, Xiaoyun Qu, Aihua Xu, Liguang Hao, Changlong Xu, Chuanlai Kuang, Hua |
author_sort | Sun, Maozhong |
collection | PubMed |
description | The interactions between chiral nanomaterials and organisms are still challenging and mysterious. Here, a chiral nanodevice made of yolk–shell nanoparticles tetrahedron (UYTe), centralized with upconversion nanoparticles (UCNPs), was fabricated to induce autophagy in vivo. The proposed chiral nanodevice displayed a tunable circular dichroism (CD) signal when modified with different enantiomers of glutathione (GSH). Notably, UYTe showed significant chirality-dependent autophagy-inducing ability after d-GSH-modification because the enhanced oxidative stress and accumulation in living cell. The activation of autophagy resulted in the reduced intracellular CD intensity from the disassembly of the structure. The intracellular ATP concentration was simultaneously enhanced in response to autophagy activity, which was quantitatively bio-imaged with the upconversion luminescence (UCL) signal of the UCNP that escaped from UYTe. The autophagy effect induced in vivo by the chiral UYTe was also visualized with UCL imaging, demonstrating the great potential utility of the chiral nanostructure for cellular biological applications. |
format | Online Article Text |
id | pubmed-6206072 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-62060722018-10-31 Direct observation of selective autophagy induction in cells and tissues by self-assembled chiral nanodevice Sun, Maozhong Hao, Tiantian Li, Xiaoyun Qu, Aihua Xu, Liguang Hao, Changlong Xu, Chuanlai Kuang, Hua Nat Commun Article The interactions between chiral nanomaterials and organisms are still challenging and mysterious. Here, a chiral nanodevice made of yolk–shell nanoparticles tetrahedron (UYTe), centralized with upconversion nanoparticles (UCNPs), was fabricated to induce autophagy in vivo. The proposed chiral nanodevice displayed a tunable circular dichroism (CD) signal when modified with different enantiomers of glutathione (GSH). Notably, UYTe showed significant chirality-dependent autophagy-inducing ability after d-GSH-modification because the enhanced oxidative stress and accumulation in living cell. The activation of autophagy resulted in the reduced intracellular CD intensity from the disassembly of the structure. The intracellular ATP concentration was simultaneously enhanced in response to autophagy activity, which was quantitatively bio-imaged with the upconversion luminescence (UCL) signal of the UCNP that escaped from UYTe. The autophagy effect induced in vivo by the chiral UYTe was also visualized with UCL imaging, demonstrating the great potential utility of the chiral nanostructure for cellular biological applications. Nature Publishing Group UK 2018-10-29 /pmc/articles/PMC6206072/ /pubmed/30374052 http://dx.doi.org/10.1038/s41467-018-06946-z Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Sun, Maozhong Hao, Tiantian Li, Xiaoyun Qu, Aihua Xu, Liguang Hao, Changlong Xu, Chuanlai Kuang, Hua Direct observation of selective autophagy induction in cells and tissues by self-assembled chiral nanodevice |
title | Direct observation of selective autophagy induction in cells and tissues by self-assembled chiral nanodevice |
title_full | Direct observation of selective autophagy induction in cells and tissues by self-assembled chiral nanodevice |
title_fullStr | Direct observation of selective autophagy induction in cells and tissues by self-assembled chiral nanodevice |
title_full_unstemmed | Direct observation of selective autophagy induction in cells and tissues by self-assembled chiral nanodevice |
title_short | Direct observation of selective autophagy induction in cells and tissues by self-assembled chiral nanodevice |
title_sort | direct observation of selective autophagy induction in cells and tissues by self-assembled chiral nanodevice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6206072/ https://www.ncbi.nlm.nih.gov/pubmed/30374052 http://dx.doi.org/10.1038/s41467-018-06946-z |
work_keys_str_mv | AT sunmaozhong directobservationofselectiveautophagyinductionincellsandtissuesbyselfassembledchiralnanodevice AT haotiantian directobservationofselectiveautophagyinductionincellsandtissuesbyselfassembledchiralnanodevice AT lixiaoyun directobservationofselectiveautophagyinductionincellsandtissuesbyselfassembledchiralnanodevice AT quaihua directobservationofselectiveautophagyinductionincellsandtissuesbyselfassembledchiralnanodevice AT xuliguang directobservationofselectiveautophagyinductionincellsandtissuesbyselfassembledchiralnanodevice AT haochanglong directobservationofselectiveautophagyinductionincellsandtissuesbyselfassembledchiralnanodevice AT xuchuanlai directobservationofselectiveautophagyinductionincellsandtissuesbyselfassembledchiralnanodevice AT kuanghua directobservationofselectiveautophagyinductionincellsandtissuesbyselfassembledchiralnanodevice |