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Ultrasensitive Quantification of Cytokine Proteins in Single Lymphocytes From Human Blood Following ex-vivo Stimulation
In this study we demonstrate the feasibility of direct, quantitative measurement of cytokine proteins in single human CD8 lymphocytes from fresh peripheral blood of healthy donors following a brief ex vivo stimulation. Cytokine-secreting cells were identified using cell surface “catch” reagents and...
Autores principales: | Saxena, Ankit, Dagur, Pradeep K., Desai, Alisha, McCoy, John Philip |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6206239/ https://www.ncbi.nlm.nih.gov/pubmed/30405640 http://dx.doi.org/10.3389/fimmu.2018.02462 |
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