Development and Validation of an Effective CRISPR/Cas9 Vector for Efficiently Isolating Positive Transformants and Transgene-Free Mutants in a Wide Range of Plant Species

The CRISPR/Cas9 technique is a highly valuable tool in creating new materials for both basic and applied researches. Previously, we succeeded in effectively generating mutations in Brassica napus using an available CRISPR/Cas9 vector pKSE401, while isolation of Cas9-free mutants is laborious and ine...

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Detalles Bibliográficos
Autores principales: Tang, Ting, Yu, Xiwen, Yang, Hong, Gao, Qi, Ji, Hongtao, Wang, Yanxu, Yan, Guanbo, Peng, Yan, Luo, Huifeng, Liu, Kede, Li, Xia, Ma, Chaozhi, Kang, Chunying, Dai, Cheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Plant Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6206294/
https://www.ncbi.nlm.nih.gov/pubmed/30405669
http://dx.doi.org/10.3389/fpls.2018.01533

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