Novel neural network application for bacterial colony classification

BACKGROUND: Bacterial colony morphology is the first step of classifying the bacterial species before sending them to subsequent identification process with devices, such as VITEK 2 automated system and mass spectrometry microbial identification system. It is essential as a pre-screening process because it can greatly reduce the scope of possible bacterial species and will make the subsequent identification more specific and increase work efficiency in clinical bacteriology. But this work needs adequate clinical laboratory expertise of bacterial colony morphology, which is especially difficult for beginners to handle properly. This study presents automatic programs for bacterial colony classification task, by applying the deep convolutional neural networks (CNN), which has a widespread use of digital imaging data analysis in hospitals. The most common 18 bacterial colony classes from Peking University First Hospital were used to train this framework, and other images out of these training dataset were utilized to test the performance of this classifier. RESULTS: The feasibility of this framework was verified by the comparison between predicted result and standard bacterial category. The classification accuracy of all 18 bacteria can reach 73%, and the accuracy and specificity of each kind of bacteria can reach as high as 90%. CONCLUSIONS: The supervised neural networks we use can have more promising classification characteristics for bacterial colony pre-screening process, and the unsupervised network should have more advantages in revealing novel characteristics from pictures, which can provide some practical indications to our clinical staffs.