Use of the MALDI BioTyper system and rapid BACpro with MALDI-TOF MS for rapid identification of microorganisms causing bacterial urinary tract infection in feline urine samples

With the increasing number of cats kept as pets, opportunities to treat cats with lower urinary tract disease (LUTD) have recently increased in the clinical veterinary field. Urine samples collected from 50 cats with bacterial cystitis brought to Maeda Veterinary Hospital between August 10, 2015 and...

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Autores principales: MAEDA, Hiroto, HAYASHI, Kaori, ISHIGE, Takayuki, SUNAGAWA, Tomohiro, TANIGAWA, Shigeko, MISHINA, Mika, WATANABE, Toshifumi, SOGAWA, Kazuyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2018
Materias:
Bacteriology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6207508/
https://www.ncbi.nlm.nih.gov/pubmed/30089741
http://dx.doi.org/10.1292/jvms.18-0145
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author MAEDA, Hiroto
HAYASHI, Kaori
ISHIGE, Takayuki
SUNAGAWA, Tomohiro
TANIGAWA, Shigeko
MISHINA, Mika
WATANABE, Toshifumi
SOGAWA, Kazuyuki
author_facet MAEDA, Hiroto
HAYASHI, Kaori
ISHIGE, Takayuki
SUNAGAWA, Tomohiro
TANIGAWA, Shigeko
MISHINA, Mika
WATANABE, Toshifumi
SOGAWA, Kazuyuki
author_sort MAEDA, Hiroto
collection PubMed
description With the increasing number of cats kept as pets, opportunities to treat cats with lower urinary tract disease (LUTD) have recently increased in the clinical veterinary field. Urine samples collected from 50 cats with bacterial cystitis brought to Maeda Veterinary Hospital between August 10, 2015 and March 31, 2017 were used in the study. Sample preparation of the urine was performed using a MALDI Sepsityper kit and rapid BACpro. To identify the isolates, MALDI-TOF MS was performed on an AutoFlex TOF/TOF mass spectrometer. MALDI-TOF MS using rapid BACpro for pretreatment was found to be a quick and reliable method for identification of bacteria from infected urine, with a shortened analysis time enabling earlier and more accurate selection of antibiotics for treatment of feline LUTD.
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spelling pubmed-62075082018-11-02 Use of the MALDI BioTyper system and rapid BACpro with MALDI-TOF MS for rapid identification of microorganisms causing bacterial urinary tract infection in feline urine samples MAEDA, Hiroto HAYASHI, Kaori ISHIGE, Takayuki SUNAGAWA, Tomohiro TANIGAWA, Shigeko MISHINA, Mika WATANABE, Toshifumi SOGAWA, Kazuyuki J Vet Med Sci Bacteriology With the increasing number of cats kept as pets, opportunities to treat cats with lower urinary tract disease (LUTD) have recently increased in the clinical veterinary field. Urine samples collected from 50 cats with bacterial cystitis brought to Maeda Veterinary Hospital between August 10, 2015 and March 31, 2017 were used in the study. Sample preparation of the urine was performed using a MALDI Sepsityper kit and rapid BACpro. To identify the isolates, MALDI-TOF MS was performed on an AutoFlex TOF/TOF mass spectrometer. MALDI-TOF MS using rapid BACpro for pretreatment was found to be a quick and reliable method for identification of bacteria from infected urine, with a shortened analysis time enabling earlier and more accurate selection of antibiotics for treatment of feline LUTD. The Japanese Society of Veterinary Science 2018-08-09 2018-10 /pmc/articles/PMC6207508/ /pubmed/30089741 http://dx.doi.org/10.1292/jvms.18-0145 Text en ©2018 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Bacteriology
MAEDA, Hiroto
HAYASHI, Kaori
ISHIGE, Takayuki
SUNAGAWA, Tomohiro
TANIGAWA, Shigeko
MISHINA, Mika
WATANABE, Toshifumi
SOGAWA, Kazuyuki
Use of the MALDI BioTyper system and rapid BACpro with MALDI-TOF MS for rapid identification of microorganisms causing bacterial urinary tract infection in feline urine samples
title Use of the MALDI BioTyper system and rapid BACpro with MALDI-TOF MS for rapid identification of microorganisms causing bacterial urinary tract infection in feline urine samples
title_full Use of the MALDI BioTyper system and rapid BACpro with MALDI-TOF MS for rapid identification of microorganisms causing bacterial urinary tract infection in feline urine samples
title_fullStr Use of the MALDI BioTyper system and rapid BACpro with MALDI-TOF MS for rapid identification of microorganisms causing bacterial urinary tract infection in feline urine samples
title_full_unstemmed Use of the MALDI BioTyper system and rapid BACpro with MALDI-TOF MS for rapid identification of microorganisms causing bacterial urinary tract infection in feline urine samples
title_short Use of the MALDI BioTyper system and rapid BACpro with MALDI-TOF MS for rapid identification of microorganisms causing bacterial urinary tract infection in feline urine samples
title_sort use of the maldi biotyper system and rapid bacpro with maldi-tof ms for rapid identification of microorganisms causing bacterial urinary tract infection in feline urine samples
topic Bacteriology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6207508/
https://www.ncbi.nlm.nih.gov/pubmed/30089741
http://dx.doi.org/10.1292/jvms.18-0145
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