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Isolation of bovine foamy virus in Japan

Bovine foamy virus (BFV) is endemic in many countries, but has not been reported in Japan. A syncytium-forming virus was isolated from peripheral blood leukocytes of clinically healthy cattle on a farm in Kanagawa prefecture during a periodic epidemiological survey of viral diseases. The isolate was...

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Autores principales: HACHIYA, Yuma, KIMURA, Kumiko, OGUMA, Keisuke, ONO, Mamiko, HORIKITA, Tetsuya, SENTSUI, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6207520/
https://www.ncbi.nlm.nih.gov/pubmed/30175751
http://dx.doi.org/10.1292/jvms.18-0121
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author HACHIYA, Yuma
KIMURA, Kumiko
OGUMA, Keisuke
ONO, Mamiko
HORIKITA, Tetsuya
SENTSUI, Hiroshi
author_facet HACHIYA, Yuma
KIMURA, Kumiko
OGUMA, Keisuke
ONO, Mamiko
HORIKITA, Tetsuya
SENTSUI, Hiroshi
author_sort HACHIYA, Yuma
collection PubMed
description Bovine foamy virus (BFV) is endemic in many countries, but has not been reported in Japan. A syncytium-forming virus was isolated from peripheral blood leukocytes of clinically healthy cattle on a farm in Kanagawa prefecture during a periodic epidemiological survey of viral diseases. The isolate was propagated in primary fetal bovine muscle cells and subsequently passaged in Madin–Darby bovine kidney cells. Since the isolate appeared to be distinct from the viruses with syncytium-forming ability previously isolated in Japan, we attempted to identify it using genomic analyses and electron microscopy. A phylogenetic analysis revealed that the isolate belongs to the bovine foamy virus cluster and is highly similar to a BFV strain isolated in China. A sero-epidemiological survey was performed using agar gel immunodiffusion test with the isolated virus as the antigen, and five of the 57 cattle tested were found to be seropositive.
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spelling pubmed-62075202018-11-02 Isolation of bovine foamy virus in Japan HACHIYA, Yuma KIMURA, Kumiko OGUMA, Keisuke ONO, Mamiko HORIKITA, Tetsuya SENTSUI, Hiroshi J Vet Med Sci Virology Bovine foamy virus (BFV) is endemic in many countries, but has not been reported in Japan. A syncytium-forming virus was isolated from peripheral blood leukocytes of clinically healthy cattle on a farm in Kanagawa prefecture during a periodic epidemiological survey of viral diseases. The isolate was propagated in primary fetal bovine muscle cells and subsequently passaged in Madin–Darby bovine kidney cells. Since the isolate appeared to be distinct from the viruses with syncytium-forming ability previously isolated in Japan, we attempted to identify it using genomic analyses and electron microscopy. A phylogenetic analysis revealed that the isolate belongs to the bovine foamy virus cluster and is highly similar to a BFV strain isolated in China. A sero-epidemiological survey was performed using agar gel immunodiffusion test with the isolated virus as the antigen, and five of the 57 cattle tested were found to be seropositive. The Japanese Society of Veterinary Science 2018-09-03 2018-10 /pmc/articles/PMC6207520/ /pubmed/30175751 http://dx.doi.org/10.1292/jvms.18-0121 Text en ©2018 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Virology
HACHIYA, Yuma
KIMURA, Kumiko
OGUMA, Keisuke
ONO, Mamiko
HORIKITA, Tetsuya
SENTSUI, Hiroshi
Isolation of bovine foamy virus in Japan
title Isolation of bovine foamy virus in Japan
title_full Isolation of bovine foamy virus in Japan
title_fullStr Isolation of bovine foamy virus in Japan
title_full_unstemmed Isolation of bovine foamy virus in Japan
title_short Isolation of bovine foamy virus in Japan
title_sort isolation of bovine foamy virus in japan
topic Virology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6207520/
https://www.ncbi.nlm.nih.gov/pubmed/30175751
http://dx.doi.org/10.1292/jvms.18-0121
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