Cargando…

Dynamic evolution of inverted repeats in Euglenophyta plastid genomes

Photosynthetic euglenids (Euglenophyta) are a monophyletic group of unicellular eukaryotes characterized by the presence of plastids, which arose as the result of the secondary endosymbiosis. Many Euglenophyta plastid (pt) genomes have been characterized recently, but they represented mainly one fam...

Descripción completa

Detalles Bibliográficos
Autores principales: Karnkowska, Anna, Bennett, Matthew S., Triemer, Richard E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6207741/
https://www.ncbi.nlm.nih.gov/pubmed/30375469
http://dx.doi.org/10.1038/s41598-018-34457-w
_version_ 1783366573977763840
author Karnkowska, Anna
Bennett, Matthew S.
Triemer, Richard E.
author_facet Karnkowska, Anna
Bennett, Matthew S.
Triemer, Richard E.
author_sort Karnkowska, Anna
collection PubMed
description Photosynthetic euglenids (Euglenophyta) are a monophyletic group of unicellular eukaryotes characterized by the presence of plastids, which arose as the result of the secondary endosymbiosis. Many Euglenophyta plastid (pt) genomes have been characterized recently, but they represented mainly one family – Euglenaceae. Here, we report a comparative analysis of plastid genomes from eight representatives of the family Phacaceae. Newly sequenced plastid genomes share a number of features including synteny and gene content, except for genes mat2 and mat5 encoding maturases. The observed diversity of intron number and presence/absence of maturases corroborated previously suggested correlation between the number of maturases in the pt genome and intron proliferation. Surprisingly, pt genomes of taxa belonging to Discoplastis and Lepocinclis encode two inverted repeat (IR) regions containing the rDNA operon, which are absent from the Euglenaceae. By mapping the presence/absence of IR region on the obtained phylogenomic tree, we reconstructed the most probable events in the evolution of IRs in the Euglenophyta. Our study highlights the dynamic nature of the Euglenophyta plastid genome, in particular with regards to the IR regions that underwent losses repeatedly.
format Online
Article
Text
id pubmed-6207741
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-62077412018-11-01 Dynamic evolution of inverted repeats in Euglenophyta plastid genomes Karnkowska, Anna Bennett, Matthew S. Triemer, Richard E. Sci Rep Article Photosynthetic euglenids (Euglenophyta) are a monophyletic group of unicellular eukaryotes characterized by the presence of plastids, which arose as the result of the secondary endosymbiosis. Many Euglenophyta plastid (pt) genomes have been characterized recently, but they represented mainly one family – Euglenaceae. Here, we report a comparative analysis of plastid genomes from eight representatives of the family Phacaceae. Newly sequenced plastid genomes share a number of features including synteny and gene content, except for genes mat2 and mat5 encoding maturases. The observed diversity of intron number and presence/absence of maturases corroborated previously suggested correlation between the number of maturases in the pt genome and intron proliferation. Surprisingly, pt genomes of taxa belonging to Discoplastis and Lepocinclis encode two inverted repeat (IR) regions containing the rDNA operon, which are absent from the Euglenaceae. By mapping the presence/absence of IR region on the obtained phylogenomic tree, we reconstructed the most probable events in the evolution of IRs in the Euglenophyta. Our study highlights the dynamic nature of the Euglenophyta plastid genome, in particular with regards to the IR regions that underwent losses repeatedly. Nature Publishing Group UK 2018-10-30 /pmc/articles/PMC6207741/ /pubmed/30375469 http://dx.doi.org/10.1038/s41598-018-34457-w Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Karnkowska, Anna
Bennett, Matthew S.
Triemer, Richard E.
Dynamic evolution of inverted repeats in Euglenophyta plastid genomes
title Dynamic evolution of inverted repeats in Euglenophyta plastid genomes
title_full Dynamic evolution of inverted repeats in Euglenophyta plastid genomes
title_fullStr Dynamic evolution of inverted repeats in Euglenophyta plastid genomes
title_full_unstemmed Dynamic evolution of inverted repeats in Euglenophyta plastid genomes
title_short Dynamic evolution of inverted repeats in Euglenophyta plastid genomes
title_sort dynamic evolution of inverted repeats in euglenophyta plastid genomes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6207741/
https://www.ncbi.nlm.nih.gov/pubmed/30375469
http://dx.doi.org/10.1038/s41598-018-34457-w
work_keys_str_mv AT karnkowskaanna dynamicevolutionofinvertedrepeatsineuglenophytaplastidgenomes
AT bennettmatthews dynamicevolutionofinvertedrepeatsineuglenophytaplastidgenomes
AT triemerricharde dynamicevolutionofinvertedrepeatsineuglenophytaplastidgenomes