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Effect of biocompatible nucleants in rapid crystallization of natural amino acids using a CW Nd:YAG laser

Laser-induced crystallization is emerging as an alternative technique to crystallize biomolecules. However, its applications are limited to specific small molecules and some simple proteins, possibly because of the need to use high-intensity, pulsed lasers and relatively long laser irradiation time....

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Autores principales: Thippeshappa, Shilpa, George, Sajan D., Bankapur, Aseefhali, Chidangil, Santhosh, Mathur, Deepak, Abdul Salam, Abdul Ajees
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6207789/
https://www.ncbi.nlm.nih.gov/pubmed/30375443
http://dx.doi.org/10.1038/s41598-018-34356-0
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author Thippeshappa, Shilpa
George, Sajan D.
Bankapur, Aseefhali
Chidangil, Santhosh
Mathur, Deepak
Abdul Salam, Abdul Ajees
author_facet Thippeshappa, Shilpa
George, Sajan D.
Bankapur, Aseefhali
Chidangil, Santhosh
Mathur, Deepak
Abdul Salam, Abdul Ajees
author_sort Thippeshappa, Shilpa
collection PubMed
description Laser-induced crystallization is emerging as an alternative technique to crystallize biomolecules. However, its applications are limited to specific small molecules and some simple proteins, possibly because of the need to use high-intensity, pulsed lasers and relatively long laser irradiation time. Both these factors tend to denature biological molecules. If the laser-intensity and time required to crystallize biomolecules were to be reduced, laser-induced crystallization may well become of widespread utility. We report here the crystallization of nineteen natural amino acids by a laser-induced method in combination with one of three nucleants: aluminum, coconut coir, and peacock feather barbule. We have utilized a low-power, continuous wave (CW) Nd:YAG laser (λ = 1064 nm). The advantages of our method are (i) the use of very small laser powers (60 mW), and (ii) the ability to obtain diffraction quality crystals within a mere few seconds. For most amino acids our method yields several orders of magnitude reduction in crystallization time. The use of biocompatible nucleants like coir fibres and peacock feather barbules are novel; their non-toxic nature may find broad applicability in rapid crystallization of diverse biological molecules.
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spelling pubmed-62077892018-11-01 Effect of biocompatible nucleants in rapid crystallization of natural amino acids using a CW Nd:YAG laser Thippeshappa, Shilpa George, Sajan D. Bankapur, Aseefhali Chidangil, Santhosh Mathur, Deepak Abdul Salam, Abdul Ajees Sci Rep Article Laser-induced crystallization is emerging as an alternative technique to crystallize biomolecules. However, its applications are limited to specific small molecules and some simple proteins, possibly because of the need to use high-intensity, pulsed lasers and relatively long laser irradiation time. Both these factors tend to denature biological molecules. If the laser-intensity and time required to crystallize biomolecules were to be reduced, laser-induced crystallization may well become of widespread utility. We report here the crystallization of nineteen natural amino acids by a laser-induced method in combination with one of three nucleants: aluminum, coconut coir, and peacock feather barbule. We have utilized a low-power, continuous wave (CW) Nd:YAG laser (λ = 1064 nm). The advantages of our method are (i) the use of very small laser powers (60 mW), and (ii) the ability to obtain diffraction quality crystals within a mere few seconds. For most amino acids our method yields several orders of magnitude reduction in crystallization time. The use of biocompatible nucleants like coir fibres and peacock feather barbules are novel; their non-toxic nature may find broad applicability in rapid crystallization of diverse biological molecules. Nature Publishing Group UK 2018-10-30 /pmc/articles/PMC6207789/ /pubmed/30375443 http://dx.doi.org/10.1038/s41598-018-34356-0 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Thippeshappa, Shilpa
George, Sajan D.
Bankapur, Aseefhali
Chidangil, Santhosh
Mathur, Deepak
Abdul Salam, Abdul Ajees
Effect of biocompatible nucleants in rapid crystallization of natural amino acids using a CW Nd:YAG laser
title Effect of biocompatible nucleants in rapid crystallization of natural amino acids using a CW Nd:YAG laser
title_full Effect of biocompatible nucleants in rapid crystallization of natural amino acids using a CW Nd:YAG laser
title_fullStr Effect of biocompatible nucleants in rapid crystallization of natural amino acids using a CW Nd:YAG laser
title_full_unstemmed Effect of biocompatible nucleants in rapid crystallization of natural amino acids using a CW Nd:YAG laser
title_short Effect of biocompatible nucleants in rapid crystallization of natural amino acids using a CW Nd:YAG laser
title_sort effect of biocompatible nucleants in rapid crystallization of natural amino acids using a cw nd:yag laser
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6207789/
https://www.ncbi.nlm.nih.gov/pubmed/30375443
http://dx.doi.org/10.1038/s41598-018-34356-0
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