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Biomimetic characteristics of mussel adhesive protein-loaded collagen membrane in guided bone regeneration of rabbit calvarial defects
PURPOSE: The aim of the present study was to evaluate the biocompatibility and barrier function of mussel adhesive protein (MAP)-loaded collagen membranes in guided bone regeneration (GBR). METHODS: Eight male New Zealand white rabbits were used. Four circular defects (diameter: 8 mm) were created i...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Korean Academy of Periodontology
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6207793/ https://www.ncbi.nlm.nih.gov/pubmed/30405938 http://dx.doi.org/10.5051/jpis.2018.48.5.305 |
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author | Song, Woong-Kyu Kang, Joo-Hyun Cha, Jae-Kook Lee, Jung-Seok Paik, Jeong-Won Jung, Ui-Won Kim, Byung-Hoon Choi, Seong-Ho |
author_facet | Song, Woong-Kyu Kang, Joo-Hyun Cha, Jae-Kook Lee, Jung-Seok Paik, Jeong-Won Jung, Ui-Won Kim, Byung-Hoon Choi, Seong-Ho |
author_sort | Song, Woong-Kyu |
collection | PubMed |
description | PURPOSE: The aim of the present study was to evaluate the biocompatibility and barrier function of mussel adhesive protein (MAP)-loaded collagen membranes in guided bone regeneration (GBR). METHODS: Eight male New Zealand white rabbits were used. Four circular defects (diameter: 8 mm) were created in the calvarium of each animal. The defects were randomly assigned to 1) a negative control group, 2) a cyanoacrylate (CA)-loaded collagen membrane group (the CA group), 3) a MAP-loaded collagen membrane group (the MAP group), and 4) a group that received a polycaprolactone block with MAP-loaded collagen membrane (the MAP-PCL group). Specimens were harvested at 2 weeks (n=4) and 8 weeks (n=4) postoperatively for observational histology and histometric analysis. RESULTS: In the histologic analysis, MAP was completely absorbed without any byproducts. In contrast, some of the CA adhesive remained, showing an inflammatory reaction, at 8 weeks. In the MAP-PCL group, the MAP-loaded collagen membranes served as a barrier membrane despite their fast degradation in GBR. No significant difference was found in the amount of new bone between the MAP-PCL and MAP groups (1.82±0.86 mm(2) and 2.60±0.65 mm(2), respectively). CONCLUSIONS: The MAP-loaded collagen membrane functioned efficiently in this rabbit calvarial GBR model, with excellent biocompatibility. Further research is needed to assess clinical applications in defect types that are more challenging for GBR than those used in the current model. |
format | Online Article Text |
id | pubmed-6207793 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Korean Academy of Periodontology |
record_format | MEDLINE/PubMed |
spelling | pubmed-62077932018-11-07 Biomimetic characteristics of mussel adhesive protein-loaded collagen membrane in guided bone regeneration of rabbit calvarial defects Song, Woong-Kyu Kang, Joo-Hyun Cha, Jae-Kook Lee, Jung-Seok Paik, Jeong-Won Jung, Ui-Won Kim, Byung-Hoon Choi, Seong-Ho J Periodontal Implant Sci Research Article PURPOSE: The aim of the present study was to evaluate the biocompatibility and barrier function of mussel adhesive protein (MAP)-loaded collagen membranes in guided bone regeneration (GBR). METHODS: Eight male New Zealand white rabbits were used. Four circular defects (diameter: 8 mm) were created in the calvarium of each animal. The defects were randomly assigned to 1) a negative control group, 2) a cyanoacrylate (CA)-loaded collagen membrane group (the CA group), 3) a MAP-loaded collagen membrane group (the MAP group), and 4) a group that received a polycaprolactone block with MAP-loaded collagen membrane (the MAP-PCL group). Specimens were harvested at 2 weeks (n=4) and 8 weeks (n=4) postoperatively for observational histology and histometric analysis. RESULTS: In the histologic analysis, MAP was completely absorbed without any byproducts. In contrast, some of the CA adhesive remained, showing an inflammatory reaction, at 8 weeks. In the MAP-PCL group, the MAP-loaded collagen membranes served as a barrier membrane despite their fast degradation in GBR. No significant difference was found in the amount of new bone between the MAP-PCL and MAP groups (1.82±0.86 mm(2) and 2.60±0.65 mm(2), respectively). CONCLUSIONS: The MAP-loaded collagen membrane functioned efficiently in this rabbit calvarial GBR model, with excellent biocompatibility. Further research is needed to assess clinical applications in defect types that are more challenging for GBR than those used in the current model. Korean Academy of Periodontology 2018-10-24 /pmc/articles/PMC6207793/ /pubmed/30405938 http://dx.doi.org/10.5051/jpis.2018.48.5.305 Text en Copyright © 2018. Korean Academy of Periodontology https://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/). |
spellingShingle | Research Article Song, Woong-Kyu Kang, Joo-Hyun Cha, Jae-Kook Lee, Jung-Seok Paik, Jeong-Won Jung, Ui-Won Kim, Byung-Hoon Choi, Seong-Ho Biomimetic characteristics of mussel adhesive protein-loaded collagen membrane in guided bone regeneration of rabbit calvarial defects |
title | Biomimetic characteristics of mussel adhesive protein-loaded collagen membrane in guided bone regeneration of rabbit calvarial defects |
title_full | Biomimetic characteristics of mussel adhesive protein-loaded collagen membrane in guided bone regeneration of rabbit calvarial defects |
title_fullStr | Biomimetic characteristics of mussel adhesive protein-loaded collagen membrane in guided bone regeneration of rabbit calvarial defects |
title_full_unstemmed | Biomimetic characteristics of mussel adhesive protein-loaded collagen membrane in guided bone regeneration of rabbit calvarial defects |
title_short | Biomimetic characteristics of mussel adhesive protein-loaded collagen membrane in guided bone regeneration of rabbit calvarial defects |
title_sort | biomimetic characteristics of mussel adhesive protein-loaded collagen membrane in guided bone regeneration of rabbit calvarial defects |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6207793/ https://www.ncbi.nlm.nih.gov/pubmed/30405938 http://dx.doi.org/10.5051/jpis.2018.48.5.305 |
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