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Differential Intestinal Mucosa Transcriptomic Biomarkers for Crohn's Disease and Ulcerative Colitis

Genetic research has shaped the inflammatory bowel disease (IBD) landscape identifying nearly two hundred risk loci. Nonetheless, the identified variants rendered only a partial success in providing criteria for the differential diagnosis between ulcerative colitis (UC) and Crohn's disease (CD)...

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Autores principales: Dobre, Maria, Milanesi, Elena, Mănuc, Teodora Ecaterina, Arsene, Dorel Eugen, Ţieranu, Cristian George, Maj, Carlo, Becheanu, Gabriel, Mănuc, Mircea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6207860/
https://www.ncbi.nlm.nih.gov/pubmed/30417021
http://dx.doi.org/10.1155/2018/9208274
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author Dobre, Maria
Milanesi, Elena
Mănuc, Teodora Ecaterina
Arsene, Dorel Eugen
Ţieranu, Cristian George
Maj, Carlo
Becheanu, Gabriel
Mănuc, Mircea
author_facet Dobre, Maria
Milanesi, Elena
Mănuc, Teodora Ecaterina
Arsene, Dorel Eugen
Ţieranu, Cristian George
Maj, Carlo
Becheanu, Gabriel
Mănuc, Mircea
author_sort Dobre, Maria
collection PubMed
description Genetic research has shaped the inflammatory bowel disease (IBD) landscape identifying nearly two hundred risk loci. Nonetheless, the identified variants rendered only a partial success in providing criteria for the differential diagnosis between ulcerative colitis (UC) and Crohn's disease (CD). Transcript levels from affected intestinal mucosa may serve as tentative biomarkers for improving classification and diagnosis of IBD. The aim of our study was to identify gene expression profiles specific for UC and CD, in endoscopically affected and normal intestinal colonic mucosa from IBD patients. We evaluated a panel of 84 genes related to the IBD-inflammatory pathway on 21 UC and 22 CD paired inflamed and not inflamed mucosa and on age-matched normal mucosa from 21 non-IBD controls. Two genes in UC (CCL11 and MMP10) and two in CD (C4BPB and IL1RN) showed an upregulation trend in both noninflamed and inflamed mucosa compared to controls. Our results suggest that the transcript levels of CCL11, MMP10, C4BPB, and IL1RN are candidate biomarkers that could help in clinical practice for the differential diagnosis between UC and CD and could guide new research on future therapeutic targets.
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spelling pubmed-62078602018-11-11 Differential Intestinal Mucosa Transcriptomic Biomarkers for Crohn's Disease and Ulcerative Colitis Dobre, Maria Milanesi, Elena Mănuc, Teodora Ecaterina Arsene, Dorel Eugen Ţieranu, Cristian George Maj, Carlo Becheanu, Gabriel Mănuc, Mircea J Immunol Res Research Article Genetic research has shaped the inflammatory bowel disease (IBD) landscape identifying nearly two hundred risk loci. Nonetheless, the identified variants rendered only a partial success in providing criteria for the differential diagnosis between ulcerative colitis (UC) and Crohn's disease (CD). Transcript levels from affected intestinal mucosa may serve as tentative biomarkers for improving classification and diagnosis of IBD. The aim of our study was to identify gene expression profiles specific for UC and CD, in endoscopically affected and normal intestinal colonic mucosa from IBD patients. We evaluated a panel of 84 genes related to the IBD-inflammatory pathway on 21 UC and 22 CD paired inflamed and not inflamed mucosa and on age-matched normal mucosa from 21 non-IBD controls. Two genes in UC (CCL11 and MMP10) and two in CD (C4BPB and IL1RN) showed an upregulation trend in both noninflamed and inflamed mucosa compared to controls. Our results suggest that the transcript levels of CCL11, MMP10, C4BPB, and IL1RN are candidate biomarkers that could help in clinical practice for the differential diagnosis between UC and CD and could guide new research on future therapeutic targets. Hindawi 2018-10-17 /pmc/articles/PMC6207860/ /pubmed/30417021 http://dx.doi.org/10.1155/2018/9208274 Text en Copyright © 2018 Maria Dobre et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Dobre, Maria
Milanesi, Elena
Mănuc, Teodora Ecaterina
Arsene, Dorel Eugen
Ţieranu, Cristian George
Maj, Carlo
Becheanu, Gabriel
Mănuc, Mircea
Differential Intestinal Mucosa Transcriptomic Biomarkers for Crohn's Disease and Ulcerative Colitis
title Differential Intestinal Mucosa Transcriptomic Biomarkers for Crohn's Disease and Ulcerative Colitis
title_full Differential Intestinal Mucosa Transcriptomic Biomarkers for Crohn's Disease and Ulcerative Colitis
title_fullStr Differential Intestinal Mucosa Transcriptomic Biomarkers for Crohn's Disease and Ulcerative Colitis
title_full_unstemmed Differential Intestinal Mucosa Transcriptomic Biomarkers for Crohn's Disease and Ulcerative Colitis
title_short Differential Intestinal Mucosa Transcriptomic Biomarkers for Crohn's Disease and Ulcerative Colitis
title_sort differential intestinal mucosa transcriptomic biomarkers for crohn's disease and ulcerative colitis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6207860/
https://www.ncbi.nlm.nih.gov/pubmed/30417021
http://dx.doi.org/10.1155/2018/9208274
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