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Cervicovaginal Fungi and Bacteria Associated With Cervical Intraepithelial Neoplasia and High-Risk Human Papillomavirus Infections in a Hispanic Population

The human cervicovaginal microbiota resides at an interface between the host and the environment and may affect susceptibility to disease. Puerto Rican women have high human papillomavirus (HPV) infection and cervical cancer rates. We hypothesized that the population structure of the cervicovaginal...

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Autores principales: Godoy-Vitorino, Filipa, Romaguera, Josefina, Zhao, Chunyu, Vargas-Robles, Daniela, Ortiz-Morales, Gilmary, Vázquez-Sánchez, Frances, Sanchez-Vázquez, Maria, de la Garza-Casillas, Manuel, Martinez-Ferrer, Magaly, White, James Robert, Bittinger, Kyle, Dominguez-Bello, Maria Gloria, Blaser, Martin J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6208322/
https://www.ncbi.nlm.nih.gov/pubmed/30405584
http://dx.doi.org/10.3389/fmicb.2018.02533
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author Godoy-Vitorino, Filipa
Romaguera, Josefina
Zhao, Chunyu
Vargas-Robles, Daniela
Ortiz-Morales, Gilmary
Vázquez-Sánchez, Frances
Sanchez-Vázquez, Maria
de la Garza-Casillas, Manuel
Martinez-Ferrer, Magaly
White, James Robert
Bittinger, Kyle
Dominguez-Bello, Maria Gloria
Blaser, Martin J.
author_facet Godoy-Vitorino, Filipa
Romaguera, Josefina
Zhao, Chunyu
Vargas-Robles, Daniela
Ortiz-Morales, Gilmary
Vázquez-Sánchez, Frances
Sanchez-Vázquez, Maria
de la Garza-Casillas, Manuel
Martinez-Ferrer, Magaly
White, James Robert
Bittinger, Kyle
Dominguez-Bello, Maria Gloria
Blaser, Martin J.
author_sort Godoy-Vitorino, Filipa
collection PubMed
description The human cervicovaginal microbiota resides at an interface between the host and the environment and may affect susceptibility to disease. Puerto Rican women have high human papillomavirus (HPV) infection and cervical cancer rates. We hypothesized that the population structure of the cervicovaginal bacterial and fungal biota changed with cervical squamous intraepithelial lesions and HPV infections. DNA was extracted from cervix, introitus, and anal sites of 62 patients attending high-risk San Juan clinics. The 16S rRNA V4 region and ITS-2 fungal regions were amplified and sequenced using Illumina technology. HPV genotyping was determined by reverse hybridization with the HPV SPF10-LiPA25 kit. HPV prevalence was 84% of which ∼44% subjects were infected with high-risk HPV, ∼35% were co-infected with as many as 9 HPV types and ∼5% were infected with exclusively low-risk HPV types. HPV diversity did not change with cervical dysplasia. Cervical bacteria were more diverse in patients with CIN3 pre-cancerous lesions. We found enrichment of Atopobium vaginae and Gardnerella vaginalis in patients with CIN3 lesions. We found no significant bacterial biomarkers associated with HPV infections. Fungal diversity was significantly higher in cervical samples with high-risk HPV and introitus samples of patients with Atypical Squamous Cells of Undetermined Significance (ASCUS). Fungal biomarker signatures for vagina and cervix include Sporidiobolaceae and Sacharomyces for ASCUS, and Malassezia for high-risk HPV infections. Our combined data suggests that specific cervicovaginal bacterial and fungal populations are related to the host epithelial microenvironment, and could play roles in cervical dysplasia.
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spelling pubmed-62083222018-11-07 Cervicovaginal Fungi and Bacteria Associated With Cervical Intraepithelial Neoplasia and High-Risk Human Papillomavirus Infections in a Hispanic Population Godoy-Vitorino, Filipa Romaguera, Josefina Zhao, Chunyu Vargas-Robles, Daniela Ortiz-Morales, Gilmary Vázquez-Sánchez, Frances Sanchez-Vázquez, Maria de la Garza-Casillas, Manuel Martinez-Ferrer, Magaly White, James Robert Bittinger, Kyle Dominguez-Bello, Maria Gloria Blaser, Martin J. Front Microbiol Microbiology The human cervicovaginal microbiota resides at an interface between the host and the environment and may affect susceptibility to disease. Puerto Rican women have high human papillomavirus (HPV) infection and cervical cancer rates. We hypothesized that the population structure of the cervicovaginal bacterial and fungal biota changed with cervical squamous intraepithelial lesions and HPV infections. DNA was extracted from cervix, introitus, and anal sites of 62 patients attending high-risk San Juan clinics. The 16S rRNA V4 region and ITS-2 fungal regions were amplified and sequenced using Illumina technology. HPV genotyping was determined by reverse hybridization with the HPV SPF10-LiPA25 kit. HPV prevalence was 84% of which ∼44% subjects were infected with high-risk HPV, ∼35% were co-infected with as many as 9 HPV types and ∼5% were infected with exclusively low-risk HPV types. HPV diversity did not change with cervical dysplasia. Cervical bacteria were more diverse in patients with CIN3 pre-cancerous lesions. We found enrichment of Atopobium vaginae and Gardnerella vaginalis in patients with CIN3 lesions. We found no significant bacterial biomarkers associated with HPV infections. Fungal diversity was significantly higher in cervical samples with high-risk HPV and introitus samples of patients with Atypical Squamous Cells of Undetermined Significance (ASCUS). Fungal biomarker signatures for vagina and cervix include Sporidiobolaceae and Sacharomyces for ASCUS, and Malassezia for high-risk HPV infections. Our combined data suggests that specific cervicovaginal bacterial and fungal populations are related to the host epithelial microenvironment, and could play roles in cervical dysplasia. Frontiers Media S.A. 2018-10-23 /pmc/articles/PMC6208322/ /pubmed/30405584 http://dx.doi.org/10.3389/fmicb.2018.02533 Text en Copyright © 2018 Godoy-Vitorino, Romaguera, Zhao, Vargas-Robles, Ortiz-Morales, Vázquez-Sánchez, Sanchez-Vázquez, de la Garza-Casillas, Martinez-Ferrer, White, Bittinger, Dominguez-Bello and Blaser. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Godoy-Vitorino, Filipa
Romaguera, Josefina
Zhao, Chunyu
Vargas-Robles, Daniela
Ortiz-Morales, Gilmary
Vázquez-Sánchez, Frances
Sanchez-Vázquez, Maria
de la Garza-Casillas, Manuel
Martinez-Ferrer, Magaly
White, James Robert
Bittinger, Kyle
Dominguez-Bello, Maria Gloria
Blaser, Martin J.
Cervicovaginal Fungi and Bacteria Associated With Cervical Intraepithelial Neoplasia and High-Risk Human Papillomavirus Infections in a Hispanic Population
title Cervicovaginal Fungi and Bacteria Associated With Cervical Intraepithelial Neoplasia and High-Risk Human Papillomavirus Infections in a Hispanic Population
title_full Cervicovaginal Fungi and Bacteria Associated With Cervical Intraepithelial Neoplasia and High-Risk Human Papillomavirus Infections in a Hispanic Population
title_fullStr Cervicovaginal Fungi and Bacteria Associated With Cervical Intraepithelial Neoplasia and High-Risk Human Papillomavirus Infections in a Hispanic Population
title_full_unstemmed Cervicovaginal Fungi and Bacteria Associated With Cervical Intraepithelial Neoplasia and High-Risk Human Papillomavirus Infections in a Hispanic Population
title_short Cervicovaginal Fungi and Bacteria Associated With Cervical Intraepithelial Neoplasia and High-Risk Human Papillomavirus Infections in a Hispanic Population
title_sort cervicovaginal fungi and bacteria associated with cervical intraepithelial neoplasia and high-risk human papillomavirus infections in a hispanic population
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6208322/
https://www.ncbi.nlm.nih.gov/pubmed/30405584
http://dx.doi.org/10.3389/fmicb.2018.02533
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