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Apoptotic Effect of Melittin Purified from Iranian Honey Bee Venom on Human Cervical Cancer HeLa Cell Line

Melittin, an amphipathic 26-residue peptide, is the main component of honey bee venom. Studies have been demonstrated that melittin has an inhibitory effect on proliferation of cancer cells. However, the precise mechanism of action is not completely understood. In the present study we have shown tha...

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Autores principales: Zarrinnahad, Hannaneh, Mahmoodzadeh, Amir, Hamidi, Monireh Parviz, Mahdavi, Mehdi, Moradi, Ali, Bagheri, Kamran Pooshang, Shahbazzadeh, Delavar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6208649/
https://www.ncbi.nlm.nih.gov/pubmed/30416405
http://dx.doi.org/10.1007/s10989-017-9641-1
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author Zarrinnahad, Hannaneh
Mahmoodzadeh, Amir
Hamidi, Monireh Parviz
Mahdavi, Mehdi
Moradi, Ali
Bagheri, Kamran Pooshang
Shahbazzadeh, Delavar
author_facet Zarrinnahad, Hannaneh
Mahmoodzadeh, Amir
Hamidi, Monireh Parviz
Mahdavi, Mehdi
Moradi, Ali
Bagheri, Kamran Pooshang
Shahbazzadeh, Delavar
author_sort Zarrinnahad, Hannaneh
collection PubMed
description Melittin, an amphipathic 26-residue peptide, is the main component of honey bee venom. Studies have been demonstrated that melittin has an inhibitory effect on proliferation of cancer cells. However, the precise mechanism of action is not completely understood. In the present study we have shown that purified melittin from Iranian honey bee venom shows anti-cancer effects on human cervical cancer cell line through induction of apoptosis. The venom was collected from Iranian honey bee (Apis mellifera meda) and melittin isolated using reversed phase HPLC. Biological activity of melittin was analyzed by hemolytic test on human red blood cells. In order to investigate whether melittin inhibits proliferation of cervical cancer cells, the viability of the melittin treated HeLa cell line was measured via MTT assay. Finally, cell death analysis was performed using Propidum iodide and Annexin V-FITC dual staining. The results showed that the half hemolytic concentration (HD50) induced by mellitin was 0.5 µg/ml in free FBS solution. IC50 obtained after 12 h at 1.8 µg/ml by MTT assay. According to flow cytometric analysis, melittin induced apoptosis at concentrations more than 1 µg/ml. These results suggest that melittin induces apoptotic cell death in cervical cancerous cells as observed by flow cytometric assay. It is concluded that melittin could be regarded as a potential candidate in future studies to discovery of new anticancer agents.
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spelling pubmed-62086492018-11-09 Apoptotic Effect of Melittin Purified from Iranian Honey Bee Venom on Human Cervical Cancer HeLa Cell Line Zarrinnahad, Hannaneh Mahmoodzadeh, Amir Hamidi, Monireh Parviz Mahdavi, Mehdi Moradi, Ali Bagheri, Kamran Pooshang Shahbazzadeh, Delavar Int J Pept Res Ther Article Melittin, an amphipathic 26-residue peptide, is the main component of honey bee venom. Studies have been demonstrated that melittin has an inhibitory effect on proliferation of cancer cells. However, the precise mechanism of action is not completely understood. In the present study we have shown that purified melittin from Iranian honey bee venom shows anti-cancer effects on human cervical cancer cell line through induction of apoptosis. The venom was collected from Iranian honey bee (Apis mellifera meda) and melittin isolated using reversed phase HPLC. Biological activity of melittin was analyzed by hemolytic test on human red blood cells. In order to investigate whether melittin inhibits proliferation of cervical cancer cells, the viability of the melittin treated HeLa cell line was measured via MTT assay. Finally, cell death analysis was performed using Propidum iodide and Annexin V-FITC dual staining. The results showed that the half hemolytic concentration (HD50) induced by mellitin was 0.5 µg/ml in free FBS solution. IC50 obtained after 12 h at 1.8 µg/ml by MTT assay. According to flow cytometric analysis, melittin induced apoptosis at concentrations more than 1 µg/ml. These results suggest that melittin induces apoptotic cell death in cervical cancerous cells as observed by flow cytometric assay. It is concluded that melittin could be regarded as a potential candidate in future studies to discovery of new anticancer agents. Springer Netherlands 2017-11-11 2018 /pmc/articles/PMC6208649/ /pubmed/30416405 http://dx.doi.org/10.1007/s10989-017-9641-1 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Article
Zarrinnahad, Hannaneh
Mahmoodzadeh, Amir
Hamidi, Monireh Parviz
Mahdavi, Mehdi
Moradi, Ali
Bagheri, Kamran Pooshang
Shahbazzadeh, Delavar
Apoptotic Effect of Melittin Purified from Iranian Honey Bee Venom on Human Cervical Cancer HeLa Cell Line
title Apoptotic Effect of Melittin Purified from Iranian Honey Bee Venom on Human Cervical Cancer HeLa Cell Line
title_full Apoptotic Effect of Melittin Purified from Iranian Honey Bee Venom on Human Cervical Cancer HeLa Cell Line
title_fullStr Apoptotic Effect of Melittin Purified from Iranian Honey Bee Venom on Human Cervical Cancer HeLa Cell Line
title_full_unstemmed Apoptotic Effect of Melittin Purified from Iranian Honey Bee Venom on Human Cervical Cancer HeLa Cell Line
title_short Apoptotic Effect of Melittin Purified from Iranian Honey Bee Venom on Human Cervical Cancer HeLa Cell Line
title_sort apoptotic effect of melittin purified from iranian honey bee venom on human cervical cancer hela cell line
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6208649/
https://www.ncbi.nlm.nih.gov/pubmed/30416405
http://dx.doi.org/10.1007/s10989-017-9641-1
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