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Uncoupling protein 3 deficiency impairs myocardial fatty acid oxidation and contractile recovery following ischemia/reperfusion

Patients with insulin resistance and type 2 diabetes have poor cardiac outcomes following myocardial infarction (MI). The mitochondrial uncoupling protein 3 (UCP3) is down-regulated in the heart with insulin resistance. We hypothesized that decreased UCP3 levels contribute to poor cardiac recovery f...

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Autores principales: Edwards, Kristin S., Ashraf, Sadia, Lomax, Tyler M., Wiseman, Jessica M., Hall, Michael E., Gava, Fabio N., Hall, John E., Hosler, Jonathan P., Harmancey, Romain
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6208686/
https://www.ncbi.nlm.nih.gov/pubmed/30374710
http://dx.doi.org/10.1007/s00395-018-0707-9
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author Edwards, Kristin S.
Ashraf, Sadia
Lomax, Tyler M.
Wiseman, Jessica M.
Hall, Michael E.
Gava, Fabio N.
Hall, John E.
Hosler, Jonathan P.
Harmancey, Romain
author_facet Edwards, Kristin S.
Ashraf, Sadia
Lomax, Tyler M.
Wiseman, Jessica M.
Hall, Michael E.
Gava, Fabio N.
Hall, John E.
Hosler, Jonathan P.
Harmancey, Romain
author_sort Edwards, Kristin S.
collection PubMed
description Patients with insulin resistance and type 2 diabetes have poor cardiac outcomes following myocardial infarction (MI). The mitochondrial uncoupling protein 3 (UCP3) is down-regulated in the heart with insulin resistance. We hypothesized that decreased UCP3 levels contribute to poor cardiac recovery following ischemia/reperfusion (I/R). After confirming that myocardial UCP3 levels were systematically decreased by 20–49% in animal models of insulin resistance and type 2 diabetes, we genetically engineered Sprague–Dawley rats with partial loss of UCP3 (ucp3(+/−)). Wild-type littermates (ucp3(+/+)) were used as controls. Isolated working hearts from ucp3(+/−) rats were characterized by impaired recovery of cardiac power and decreased long-chain fatty acid (LCFA) oxidation following I/R. Mitochondria isolated from ucp3(+/−) hearts subjected to I/R in vivo displayed increased reactive oxygen species (ROS) generation and decreased respiratory complex I activity. Supplying ucp3(+/−) cardiac mitochondria with the medium-chain fatty acid (MCFA) octanoate slowed electron transport through the respiratory chain and reduced ROS generation. This was accompanied by improvement of cardiac LCFA oxidation and recovery of contractile function post ischemia. In conclusion, we demonstrated that normal cardiac UCP3 levels are essential to recovery of LCFA oxidation, mitochondrial respiratory capacity, and contractile function following I/R. These results reveal a potential mechanism for the poor prognosis of type 2 diabetic patients following MI and expose MCFA supplementation as a feasible metabolic intervention to improve recovery of these patients at reperfusion. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00395-018-0707-9) contains supplementary material, which is available to authorized users.
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spelling pubmed-62086862018-11-09 Uncoupling protein 3 deficiency impairs myocardial fatty acid oxidation and contractile recovery following ischemia/reperfusion Edwards, Kristin S. Ashraf, Sadia Lomax, Tyler M. Wiseman, Jessica M. Hall, Michael E. Gava, Fabio N. Hall, John E. Hosler, Jonathan P. Harmancey, Romain Basic Res Cardiol Original Contribution Patients with insulin resistance and type 2 diabetes have poor cardiac outcomes following myocardial infarction (MI). The mitochondrial uncoupling protein 3 (UCP3) is down-regulated in the heart with insulin resistance. We hypothesized that decreased UCP3 levels contribute to poor cardiac recovery following ischemia/reperfusion (I/R). After confirming that myocardial UCP3 levels were systematically decreased by 20–49% in animal models of insulin resistance and type 2 diabetes, we genetically engineered Sprague–Dawley rats with partial loss of UCP3 (ucp3(+/−)). Wild-type littermates (ucp3(+/+)) were used as controls. Isolated working hearts from ucp3(+/−) rats were characterized by impaired recovery of cardiac power and decreased long-chain fatty acid (LCFA) oxidation following I/R. Mitochondria isolated from ucp3(+/−) hearts subjected to I/R in vivo displayed increased reactive oxygen species (ROS) generation and decreased respiratory complex I activity. Supplying ucp3(+/−) cardiac mitochondria with the medium-chain fatty acid (MCFA) octanoate slowed electron transport through the respiratory chain and reduced ROS generation. This was accompanied by improvement of cardiac LCFA oxidation and recovery of contractile function post ischemia. In conclusion, we demonstrated that normal cardiac UCP3 levels are essential to recovery of LCFA oxidation, mitochondrial respiratory capacity, and contractile function following I/R. These results reveal a potential mechanism for the poor prognosis of type 2 diabetic patients following MI and expose MCFA supplementation as a feasible metabolic intervention to improve recovery of these patients at reperfusion. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00395-018-0707-9) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2018-10-29 2018 /pmc/articles/PMC6208686/ /pubmed/30374710 http://dx.doi.org/10.1007/s00395-018-0707-9 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Contribution
Edwards, Kristin S.
Ashraf, Sadia
Lomax, Tyler M.
Wiseman, Jessica M.
Hall, Michael E.
Gava, Fabio N.
Hall, John E.
Hosler, Jonathan P.
Harmancey, Romain
Uncoupling protein 3 deficiency impairs myocardial fatty acid oxidation and contractile recovery following ischemia/reperfusion
title Uncoupling protein 3 deficiency impairs myocardial fatty acid oxidation and contractile recovery following ischemia/reperfusion
title_full Uncoupling protein 3 deficiency impairs myocardial fatty acid oxidation and contractile recovery following ischemia/reperfusion
title_fullStr Uncoupling protein 3 deficiency impairs myocardial fatty acid oxidation and contractile recovery following ischemia/reperfusion
title_full_unstemmed Uncoupling protein 3 deficiency impairs myocardial fatty acid oxidation and contractile recovery following ischemia/reperfusion
title_short Uncoupling protein 3 deficiency impairs myocardial fatty acid oxidation and contractile recovery following ischemia/reperfusion
title_sort uncoupling protein 3 deficiency impairs myocardial fatty acid oxidation and contractile recovery following ischemia/reperfusion
topic Original Contribution
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6208686/
https://www.ncbi.nlm.nih.gov/pubmed/30374710
http://dx.doi.org/10.1007/s00395-018-0707-9
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