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Advancing metabolic engineering of Yarrowia lipolytica using the CRISPR/Cas system

The oleaginous yeast Yarrowia lipolytica is widely used for the production of both bulk and fine chemicals, including organic acids, fatty acid-derived biofuels and chemicals, polyunsaturated fatty acids, single-cell proteins, terpenoids, and other valuable products. Consequently, it is becoming inc...

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Autores principales: Shi, Tian-Qiong, Huang, He, Kerkhoven, Eduard J., Ji, Xiao-Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6208947/
https://www.ncbi.nlm.nih.gov/pubmed/30238143
http://dx.doi.org/10.1007/s00253-018-9366-x
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author Shi, Tian-Qiong
Huang, He
Kerkhoven, Eduard J.
Ji, Xiao-Jun
author_facet Shi, Tian-Qiong
Huang, He
Kerkhoven, Eduard J.
Ji, Xiao-Jun
author_sort Shi, Tian-Qiong
collection PubMed
description The oleaginous yeast Yarrowia lipolytica is widely used for the production of both bulk and fine chemicals, including organic acids, fatty acid-derived biofuels and chemicals, polyunsaturated fatty acids, single-cell proteins, terpenoids, and other valuable products. Consequently, it is becoming increasingly popular for metabolic engineering applications. Multiple gene manipulation tools including URA blast, Cre/LoxP, and transcription activator-like effector nucleases (TALENs) have been developed for metabolic engineering in Y. lipolytica. However, the low efficiency and time-consuming procedures involved in these methods hamper further research. The emergence of the CRISPR/Cas system offers a potential solution for these problems due to its high efficiency, ease of operation, and time savings, which can significantly accelerate the genomic engineering of Y. lipolytica. In this review, we summarize the research progress on the development of CRISPR/Cas systems for Y. lipolytica, including Cas9 proteins and sgRNA expression strategies, as well as gene knock-out/knock-in and repression/activation applications. Finally, the most promising and tantalizing future prospects in this area are highlighted.
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spelling pubmed-62089472018-11-09 Advancing metabolic engineering of Yarrowia lipolytica using the CRISPR/Cas system Shi, Tian-Qiong Huang, He Kerkhoven, Eduard J. Ji, Xiao-Jun Appl Microbiol Biotechnol Mini-Review The oleaginous yeast Yarrowia lipolytica is widely used for the production of both bulk and fine chemicals, including organic acids, fatty acid-derived biofuels and chemicals, polyunsaturated fatty acids, single-cell proteins, terpenoids, and other valuable products. Consequently, it is becoming increasingly popular for metabolic engineering applications. Multiple gene manipulation tools including URA blast, Cre/LoxP, and transcription activator-like effector nucleases (TALENs) have been developed for metabolic engineering in Y. lipolytica. However, the low efficiency and time-consuming procedures involved in these methods hamper further research. The emergence of the CRISPR/Cas system offers a potential solution for these problems due to its high efficiency, ease of operation, and time savings, which can significantly accelerate the genomic engineering of Y. lipolytica. In this review, we summarize the research progress on the development of CRISPR/Cas systems for Y. lipolytica, including Cas9 proteins and sgRNA expression strategies, as well as gene knock-out/knock-in and repression/activation applications. Finally, the most promising and tantalizing future prospects in this area are highlighted. Springer Berlin Heidelberg 2018-09-21 2018 /pmc/articles/PMC6208947/ /pubmed/30238143 http://dx.doi.org/10.1007/s00253-018-9366-x Text en © The Author(s) 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Mini-Review
Shi, Tian-Qiong
Huang, He
Kerkhoven, Eduard J.
Ji, Xiao-Jun
Advancing metabolic engineering of Yarrowia lipolytica using the CRISPR/Cas system
title Advancing metabolic engineering of Yarrowia lipolytica using the CRISPR/Cas system
title_full Advancing metabolic engineering of Yarrowia lipolytica using the CRISPR/Cas system
title_fullStr Advancing metabolic engineering of Yarrowia lipolytica using the CRISPR/Cas system
title_full_unstemmed Advancing metabolic engineering of Yarrowia lipolytica using the CRISPR/Cas system
title_short Advancing metabolic engineering of Yarrowia lipolytica using the CRISPR/Cas system
title_sort advancing metabolic engineering of yarrowia lipolytica using the crispr/cas system
topic Mini-Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6208947/
https://www.ncbi.nlm.nih.gov/pubmed/30238143
http://dx.doi.org/10.1007/s00253-018-9366-x
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