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Regulation of CATSPER1 expression by the testis-determining gene SRY
CATSPER1 gene encodes a pore-forming and pH-sensing subunit of the CatSper Ca(2+)- permeable channel, a protein in the flagellum essential for sperm hyperactivation. Previous studies have shown that the murine Catsper1 gene promoter is regulated by different Sox proteins. Likewise, it is acknowledge...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6209213/ https://www.ncbi.nlm.nih.gov/pubmed/30379860 http://dx.doi.org/10.1371/journal.pone.0205744 |
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author | Olivares, Aleida Hernández-Reyes, Adriana Felix, Ricardo Forero, Ángela Mata-Rocha, Minerva Hernández-Sánchez, Javier Santos, Isis Aguirre-Alvarado, Charmina Oviedo, Norma |
author_facet | Olivares, Aleida Hernández-Reyes, Adriana Felix, Ricardo Forero, Ángela Mata-Rocha, Minerva Hernández-Sánchez, Javier Santos, Isis Aguirre-Alvarado, Charmina Oviedo, Norma |
author_sort | Olivares, Aleida |
collection | PubMed |
description | CATSPER1 gene encodes a pore-forming and pH-sensing subunit of the CatSper Ca(2+)- permeable channel, a protein in the flagellum essential for sperm hyperactivation. Previous studies have shown that the murine Catsper1 gene promoter is regulated by different Sox proteins. Likewise, it is acknowledged that the human CATSPER1 gene promoter sequence is enriched in potential interaction sites for the sex-determining region Y gene (SRY), which suggest a novel regulatory transcriptional mechanism for CatSper1 channel expression. Therefore, in this work, we sought to determine whether the human CATSPER1 gene expression is regulated by the SRY transcription factor. To this end, a series of deletions and mutations were introduced in the wild- type CATSPER1 gene promoter to eliminate the SRY sites, and the different constructs were tested for their ability to activate transcription in human embryonic kidney and murine spermatogonial germ cell lines (HEK-293 and GC1-spg, respectively) using luciferase assays. In addition, by using a strategy that combines electrophoretic mobility shift assays (EMSA) and chromatin immunoprecipitation (ChIP) we investigated whether the CATSPER1 gene expression is regulated by the SRY transcription factor both in vitro and in vivo. Our results show that the transcriptional factor SRY specifically binds to different sites in the promoter sequence and has the ability to control CATSPER1 gene transcription. |
format | Online Article Text |
id | pubmed-6209213 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-62092132018-11-19 Regulation of CATSPER1 expression by the testis-determining gene SRY Olivares, Aleida Hernández-Reyes, Adriana Felix, Ricardo Forero, Ángela Mata-Rocha, Minerva Hernández-Sánchez, Javier Santos, Isis Aguirre-Alvarado, Charmina Oviedo, Norma PLoS One Research Article CATSPER1 gene encodes a pore-forming and pH-sensing subunit of the CatSper Ca(2+)- permeable channel, a protein in the flagellum essential for sperm hyperactivation. Previous studies have shown that the murine Catsper1 gene promoter is regulated by different Sox proteins. Likewise, it is acknowledged that the human CATSPER1 gene promoter sequence is enriched in potential interaction sites for the sex-determining region Y gene (SRY), which suggest a novel regulatory transcriptional mechanism for CatSper1 channel expression. Therefore, in this work, we sought to determine whether the human CATSPER1 gene expression is regulated by the SRY transcription factor. To this end, a series of deletions and mutations were introduced in the wild- type CATSPER1 gene promoter to eliminate the SRY sites, and the different constructs were tested for their ability to activate transcription in human embryonic kidney and murine spermatogonial germ cell lines (HEK-293 and GC1-spg, respectively) using luciferase assays. In addition, by using a strategy that combines electrophoretic mobility shift assays (EMSA) and chromatin immunoprecipitation (ChIP) we investigated whether the CATSPER1 gene expression is regulated by the SRY transcription factor both in vitro and in vivo. Our results show that the transcriptional factor SRY specifically binds to different sites in the promoter sequence and has the ability to control CATSPER1 gene transcription. Public Library of Science 2018-10-31 /pmc/articles/PMC6209213/ /pubmed/30379860 http://dx.doi.org/10.1371/journal.pone.0205744 Text en © 2018 Olivares et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Olivares, Aleida Hernández-Reyes, Adriana Felix, Ricardo Forero, Ángela Mata-Rocha, Minerva Hernández-Sánchez, Javier Santos, Isis Aguirre-Alvarado, Charmina Oviedo, Norma Regulation of CATSPER1 expression by the testis-determining gene SRY |
title | Regulation of CATSPER1 expression by the testis-determining gene SRY |
title_full | Regulation of CATSPER1 expression by the testis-determining gene SRY |
title_fullStr | Regulation of CATSPER1 expression by the testis-determining gene SRY |
title_full_unstemmed | Regulation of CATSPER1 expression by the testis-determining gene SRY |
title_short | Regulation of CATSPER1 expression by the testis-determining gene SRY |
title_sort | regulation of catsper1 expression by the testis-determining gene sry |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6209213/ https://www.ncbi.nlm.nih.gov/pubmed/30379860 http://dx.doi.org/10.1371/journal.pone.0205744 |
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