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p53 Is Active in Human Amniotic Fluid Stem Cells
Despite increasing interest in human amniotic fluid cells, very little is known about the regulation and function of p53 in this cell type. In this study, we show that undifferentiated human amniotic fluid cells express p53, yet at lower levels than in cancer cells. The p53 protein in amniotic fluid...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Mary Ann Liebert, Inc., publishers
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6209428/ https://www.ncbi.nlm.nih.gov/pubmed/30044176 http://dx.doi.org/10.1089/scd.2017.0254 |
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author | Rodrigues, Melissa Antonucci, Ivana Elabd, Seham Kancherla, Shilpa Marchisio, Marco Blattner, Christine Stuppia, Liborio |
author_facet | Rodrigues, Melissa Antonucci, Ivana Elabd, Seham Kancherla, Shilpa Marchisio, Marco Blattner, Christine Stuppia, Liborio |
author_sort | Rodrigues, Melissa |
collection | PubMed |
description | Despite increasing interest in human amniotic fluid cells, very little is known about the regulation and function of p53 in this cell type. In this study, we show that undifferentiated human amniotic fluid cells express p53, yet at lower levels than in cancer cells. The p53 protein in amniotic fluid cells is mainly localized in the nuclei, however, its antiproliferative activity is compromised in these cells. Igf2, a maternal imprinted gene, and c-jun, a proto-oncogene, are regulated by p53 in these cells. DNA damage leads to an increase in p53 abundance in human amniotic fluid cells and to transcriptional activation of its target genes. Interestingly, cell differentiation toward the neural lineage leads to p53 induction as differentiation progresses. |
format | Online Article Text |
id | pubmed-6209428 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Mary Ann Liebert, Inc., publishers |
record_format | MEDLINE/PubMed |
spelling | pubmed-62094282018-11-02 p53 Is Active in Human Amniotic Fluid Stem Cells Rodrigues, Melissa Antonucci, Ivana Elabd, Seham Kancherla, Shilpa Marchisio, Marco Blattner, Christine Stuppia, Liborio Stem Cells Dev Original Research Reports Despite increasing interest in human amniotic fluid cells, very little is known about the regulation and function of p53 in this cell type. In this study, we show that undifferentiated human amniotic fluid cells express p53, yet at lower levels than in cancer cells. The p53 protein in amniotic fluid cells is mainly localized in the nuclei, however, its antiproliferative activity is compromised in these cells. Igf2, a maternal imprinted gene, and c-jun, a proto-oncogene, are regulated by p53 in these cells. DNA damage leads to an increase in p53 abundance in human amniotic fluid cells and to transcriptional activation of its target genes. Interestingly, cell differentiation toward the neural lineage leads to p53 induction as differentiation progresses. Mary Ann Liebert, Inc., publishers 2018-11-01 2018-10-25 /pmc/articles/PMC6209428/ /pubmed/30044176 http://dx.doi.org/10.1089/scd.2017.0254 Text en © Melissa Rodrigues et al. 2018; Published by Mary Ann Liebert, Inc. This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Research Reports Rodrigues, Melissa Antonucci, Ivana Elabd, Seham Kancherla, Shilpa Marchisio, Marco Blattner, Christine Stuppia, Liborio p53 Is Active in Human Amniotic Fluid Stem Cells |
title | p53 Is Active in Human Amniotic Fluid Stem Cells |
title_full | p53 Is Active in Human Amniotic Fluid Stem Cells |
title_fullStr | p53 Is Active in Human Amniotic Fluid Stem Cells |
title_full_unstemmed | p53 Is Active in Human Amniotic Fluid Stem Cells |
title_short | p53 Is Active in Human Amniotic Fluid Stem Cells |
title_sort | p53 is active in human amniotic fluid stem cells |
topic | Original Research Reports |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6209428/ https://www.ncbi.nlm.nih.gov/pubmed/30044176 http://dx.doi.org/10.1089/scd.2017.0254 |
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