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Specific expression of lncRNA RP13-650J16.1 and TCONS_00023979 in prostate cancer
The aim of the present study was to explore the expression profile and the potential regulatory mechanism of two long non-coding RNAs (lncRNAs) (RP13-650J16.1 and TCONS_00023979) in prostate cancer (PCa). Expression profile of lncRNAs in PCa and paracancerous tissues were investgated by the high-thr...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Portland Press Ltd.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6209587/ https://www.ncbi.nlm.nih.gov/pubmed/30279207 http://dx.doi.org/10.1042/BSR20171571 |
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author | Zhou, Xiaochen Chen, Qiong Wang, Haolin Zhang, Cheng Fu, Bin Wang, Gongxian |
author_facet | Zhou, Xiaochen Chen, Qiong Wang, Haolin Zhang, Cheng Fu, Bin Wang, Gongxian |
author_sort | Zhou, Xiaochen |
collection | PubMed |
description | The aim of the present study was to explore the expression profile and the potential regulatory mechanism of two long non-coding RNAs (lncRNAs) (RP13-650J16.1 and TCONS_00023979) in prostate cancer (PCa). Expression profile of lncRNAs in PCa and paracancerous tissues were investgated by the high-throughput gene chip technology. Specific siRNA of RP13-650J16.1 or TCONS_00023979 was transfected into DU145 cells. Then, the relative expression of RP13-650J16.1, receptor-associated coactivator 3 (RAC3), promyelocytic leukemia (PML), and TCONS_00023979 was detected by quantitative real-time PCR and Western blotting. MTT assay was used to detect the proliferation of DU145 cells. The migration ability of DU145 cells was measured by Transwell chambers. Single cell proliferation and clonogenic ability were detected by plate clone formation assay. RP13-650J16.1 and RAC3 expression was up-regulated, and TCONS_00023979 and PML expression was down-regulated in PCa tissues. Silencing RP13-650J16.1 could decrease RAC3 expression, and knockout of TCONS_00023979 also reduced PML expression. Moreover, the ability of proliferation, migration, and colony formation of DU145 cells was decreased after transfected with si-RP13-650J16.1, while these abilities were increased after transfected with si-TCONS_00023979. Collectively, our findings demonstrated that RP13-650J16.1 might be an oncogene and TCONS_00023979 might be an antioncogene in PCa. |
format | Online Article Text |
id | pubmed-6209587 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-62095872018-11-14 Specific expression of lncRNA RP13-650J16.1 and TCONS_00023979 in prostate cancer Zhou, Xiaochen Chen, Qiong Wang, Haolin Zhang, Cheng Fu, Bin Wang, Gongxian Biosci Rep Research Articles The aim of the present study was to explore the expression profile and the potential regulatory mechanism of two long non-coding RNAs (lncRNAs) (RP13-650J16.1 and TCONS_00023979) in prostate cancer (PCa). Expression profile of lncRNAs in PCa and paracancerous tissues were investgated by the high-throughput gene chip technology. Specific siRNA of RP13-650J16.1 or TCONS_00023979 was transfected into DU145 cells. Then, the relative expression of RP13-650J16.1, receptor-associated coactivator 3 (RAC3), promyelocytic leukemia (PML), and TCONS_00023979 was detected by quantitative real-time PCR and Western blotting. MTT assay was used to detect the proliferation of DU145 cells. The migration ability of DU145 cells was measured by Transwell chambers. Single cell proliferation and clonogenic ability were detected by plate clone formation assay. RP13-650J16.1 and RAC3 expression was up-regulated, and TCONS_00023979 and PML expression was down-regulated in PCa tissues. Silencing RP13-650J16.1 could decrease RAC3 expression, and knockout of TCONS_00023979 also reduced PML expression. Moreover, the ability of proliferation, migration, and colony formation of DU145 cells was decreased after transfected with si-RP13-650J16.1, while these abilities were increased after transfected with si-TCONS_00023979. Collectively, our findings demonstrated that RP13-650J16.1 might be an oncogene and TCONS_00023979 might be an antioncogene in PCa. Portland Press Ltd. 2018-10-31 /pmc/articles/PMC6209587/ /pubmed/30279207 http://dx.doi.org/10.1042/BSR20171571 Text en © 2018 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Articles Zhou, Xiaochen Chen, Qiong Wang, Haolin Zhang, Cheng Fu, Bin Wang, Gongxian Specific expression of lncRNA RP13-650J16.1 and TCONS_00023979 in prostate cancer |
title | Specific expression of lncRNA RP13-650J16.1 and TCONS_00023979 in prostate cancer |
title_full | Specific expression of lncRNA RP13-650J16.1 and TCONS_00023979 in prostate cancer |
title_fullStr | Specific expression of lncRNA RP13-650J16.1 and TCONS_00023979 in prostate cancer |
title_full_unstemmed | Specific expression of lncRNA RP13-650J16.1 and TCONS_00023979 in prostate cancer |
title_short | Specific expression of lncRNA RP13-650J16.1 and TCONS_00023979 in prostate cancer |
title_sort | specific expression of lncrna rp13-650j16.1 and tcons_00023979 in prostate cancer |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6209587/ https://www.ncbi.nlm.nih.gov/pubmed/30279207 http://dx.doi.org/10.1042/BSR20171571 |
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