Cargando…

LncRNA myocardial infarction-associated transcript promotes cell proliferation and inhibits cell apoptosis by targeting miR-330-5p in epithelial ovarian cancer cells

INTRODUCTION: Long non-coding RNAs (lncRNAs) have been shown to have great importance in cancer development and progression. However, the mechanism of lncRNAs in epithelial ovarian cancer remains unclear. In the present study, we aimed to explore the role of the lncRNA myocardial infarction-associat...

Descripción completa

Detalles Bibliográficos
Autores principales: Shao, Shiqing, Tian, Jun, Zhang, Hongxia, Wang, Shelian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Termedia Publishing House 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6209713/
https://www.ncbi.nlm.nih.gov/pubmed/30393480
http://dx.doi.org/10.5114/aoms.2018.75535
_version_ 1783366953520332800
author Shao, Shiqing
Tian, Jun
Zhang, Hongxia
Wang, Shelian
author_facet Shao, Shiqing
Tian, Jun
Zhang, Hongxia
Wang, Shelian
author_sort Shao, Shiqing
collection PubMed
description INTRODUCTION: Long non-coding RNAs (lncRNAs) have been shown to have great importance in cancer development and progression. However, the mechanism of lncRNAs in epithelial ovarian cancer remains unclear. In the present study, we aimed to explore the role of the lncRNA myocardial infarction-associated transcript (MIAT) in epithelial ovarian cancer tumorigenesis. MATERIAL AND METHODS: Quantitative real-time PCR (qRT-PCR) was used to determine MIAT expression in human epithelial ovarian cancer tissues and cell lines, and the effects of MIAT on cell proliferation and cell apoptosis were determined by CCK-8 assay or flow cytometry analysis. Dual-Luciferase Reporter assay and Western blot assay were used to explore the molecular mechanisms of MIAT in epithelial ovarian cancer cells progression. RESULTS: Our data showed that the expression of lncRNA MIAT was remarkably increased in human epithelial ovarian cancer tissues and cell lines (p < 0.05). High MIAT expression was associated with poor overall survival of epithelial ovarian cancer patients (p < 0.05). Function assays showed that knockdown of MIAT expression significantly inhibited epithelial ovarian cancer cell proliferation and promoted cell apoptosis in vitro (p < 0.05). Moreover, we revealed that MIAT might function as an endogenous miR-330-5p sponge to regulate the target gene of miR-330-5p in epithelial ovarian cancer progression. CONCLUSIONS: LncRNA MIAT was found to be a tumor oncogenic lncRNA in epithelial ovarian cancer tumorigenesis. LncRNA MIAT promoted cell proliferation and inhibited cell apoptosis by negative regulation of miR-330-5p in epithelial ovarian cancer cells. Our findings suggested that MIAT might act as a candidate prognostic biomarker and new therapeutic target for treating epithelial ovarian cancer patients.
format Online
Article
Text
id pubmed-6209713
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Termedia Publishing House
record_format MEDLINE/PubMed
spelling pubmed-62097132018-11-02 LncRNA myocardial infarction-associated transcript promotes cell proliferation and inhibits cell apoptosis by targeting miR-330-5p in epithelial ovarian cancer cells Shao, Shiqing Tian, Jun Zhang, Hongxia Wang, Shelian Arch Med Sci Basic Research INTRODUCTION: Long non-coding RNAs (lncRNAs) have been shown to have great importance in cancer development and progression. However, the mechanism of lncRNAs in epithelial ovarian cancer remains unclear. In the present study, we aimed to explore the role of the lncRNA myocardial infarction-associated transcript (MIAT) in epithelial ovarian cancer tumorigenesis. MATERIAL AND METHODS: Quantitative real-time PCR (qRT-PCR) was used to determine MIAT expression in human epithelial ovarian cancer tissues and cell lines, and the effects of MIAT on cell proliferation and cell apoptosis were determined by CCK-8 assay or flow cytometry analysis. Dual-Luciferase Reporter assay and Western blot assay were used to explore the molecular mechanisms of MIAT in epithelial ovarian cancer cells progression. RESULTS: Our data showed that the expression of lncRNA MIAT was remarkably increased in human epithelial ovarian cancer tissues and cell lines (p < 0.05). High MIAT expression was associated with poor overall survival of epithelial ovarian cancer patients (p < 0.05). Function assays showed that knockdown of MIAT expression significantly inhibited epithelial ovarian cancer cell proliferation and promoted cell apoptosis in vitro (p < 0.05). Moreover, we revealed that MIAT might function as an endogenous miR-330-5p sponge to regulate the target gene of miR-330-5p in epithelial ovarian cancer progression. CONCLUSIONS: LncRNA MIAT was found to be a tumor oncogenic lncRNA in epithelial ovarian cancer tumorigenesis. LncRNA MIAT promoted cell proliferation and inhibited cell apoptosis by negative regulation of miR-330-5p in epithelial ovarian cancer cells. Our findings suggested that MIAT might act as a candidate prognostic biomarker and new therapeutic target for treating epithelial ovarian cancer patients. Termedia Publishing House 2018-04-30 2018-10 /pmc/articles/PMC6209713/ /pubmed/30393480 http://dx.doi.org/10.5114/aoms.2018.75535 Text en Copyright: © 2018 Termedia & Banach http://creativecommons.org/licenses/by-nc-sa/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) License, allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material, provided the original work is properly cited and states its license.
spellingShingle Basic Research
Shao, Shiqing
Tian, Jun
Zhang, Hongxia
Wang, Shelian
LncRNA myocardial infarction-associated transcript promotes cell proliferation and inhibits cell apoptosis by targeting miR-330-5p in epithelial ovarian cancer cells
title LncRNA myocardial infarction-associated transcript promotes cell proliferation and inhibits cell apoptosis by targeting miR-330-5p in epithelial ovarian cancer cells
title_full LncRNA myocardial infarction-associated transcript promotes cell proliferation and inhibits cell apoptosis by targeting miR-330-5p in epithelial ovarian cancer cells
title_fullStr LncRNA myocardial infarction-associated transcript promotes cell proliferation and inhibits cell apoptosis by targeting miR-330-5p in epithelial ovarian cancer cells
title_full_unstemmed LncRNA myocardial infarction-associated transcript promotes cell proliferation and inhibits cell apoptosis by targeting miR-330-5p in epithelial ovarian cancer cells
title_short LncRNA myocardial infarction-associated transcript promotes cell proliferation and inhibits cell apoptosis by targeting miR-330-5p in epithelial ovarian cancer cells
title_sort lncrna myocardial infarction-associated transcript promotes cell proliferation and inhibits cell apoptosis by targeting mir-330-5p in epithelial ovarian cancer cells
topic Basic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6209713/
https://www.ncbi.nlm.nih.gov/pubmed/30393480
http://dx.doi.org/10.5114/aoms.2018.75535
work_keys_str_mv AT shaoshiqing lncrnamyocardialinfarctionassociatedtranscriptpromotescellproliferationandinhibitscellapoptosisbytargetingmir3305pinepithelialovariancancercells
AT tianjun lncrnamyocardialinfarctionassociatedtranscriptpromotescellproliferationandinhibitscellapoptosisbytargetingmir3305pinepithelialovariancancercells
AT zhanghongxia lncrnamyocardialinfarctionassociatedtranscriptpromotescellproliferationandinhibitscellapoptosisbytargetingmir3305pinepithelialovariancancercells
AT wangshelian lncrnamyocardialinfarctionassociatedtranscriptpromotescellproliferationandinhibitscellapoptosisbytargetingmir3305pinepithelialovariancancercells