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Dietary Arginine Supplementation Affects Intestinal Function by Enhancing Antioxidant Capacity of a Nitric Oxide–Independent Pathway in Low-Birth-Weight Piglets

BACKGROUND: Low-birth-weight (LBW) neonates are susceptible to intestinal dysfunction. Furthermore, the antioxidant capacity of LBW neonates is significantly lower compared with that of normal-birth-weight (NBW) neonates both at birth and at weaning. In LBW neonates, dietary supplementation with arg...

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Autores principales: Zheng, Ping, Song, Yi, Tian, Yihang, Zhang, Hao, Yu, Bing, He, Jun, Mao, Xiangbing, Yu, Jie, Luo, Yuheng, Luo, Junqiu, Huang, Zhiqing, Tian, Gang, Chen, Hong, Chen, Daiwen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6209807/
https://www.ncbi.nlm.nih.gov/pubmed/30383283
http://dx.doi.org/10.1093/jn/nxy198
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author Zheng, Ping
Song, Yi
Tian, Yihang
Zhang, Hao
Yu, Bing
He, Jun
Mao, Xiangbing
Yu, Jie
Luo, Yuheng
Luo, Junqiu
Huang, Zhiqing
Tian, Gang
Chen, Hong
Chen, Daiwen
author_facet Zheng, Ping
Song, Yi
Tian, Yihang
Zhang, Hao
Yu, Bing
He, Jun
Mao, Xiangbing
Yu, Jie
Luo, Yuheng
Luo, Junqiu
Huang, Zhiqing
Tian, Gang
Chen, Hong
Chen, Daiwen
author_sort Zheng, Ping
collection PubMed
description BACKGROUND: Low-birth-weight (LBW) neonates are susceptible to intestinal dysfunction. Furthermore, the antioxidant capacity of LBW neonates is significantly lower compared with that of normal-birth-weight (NBW) neonates both at birth and at weaning. In LBW neonates, dietary supplementation with arginine has shown beneficial effects on intestinal function. OBJECTIVE: The present study explored the potential mechanisms of arginine-induced protective effects against intestinal dysfunction in LBW piglets. METHODS: Forty 4-d-old LBW piglets [body weight (BW): 1.05 ± 0.04 kg] (Large White × Landrace) were assigned to 4 treatments and artificially fed a whole-milk powder– and whey protein concentrate–based diet (containing 0.65% arginine) either not supplemented with arginine (LBWC) or supplemented with 0.5%, 1.0%, or 1.5% l-arginine for 21 d. In addition, 10 NBW siblings (BW: 1.96 ± 0.03 kg) were selected and fed the basal diet. Growth performance, intestinal morphology, mRNA expression of tight junction protein, redox-sensitive genes and nitric oxide (NO) synthase, cytokines, and redox indexes were determined. Data were subjected to 1-factor ANOVA. RESULTS: LBW piglets exhibited poorer growth performance (29.9%), lower Claudin1 mRNA level (63.6%), lower antioxidant capacity (22.9 ∼ 24.3%), and higher jejunum interleukin 1 (IL-1) concentration (18.8%) compared with NBW piglets. Dietary supplementation with 0.5% and 1.0% l-arginine significantly enhanced daily BW gain of LBW piglets by 13.6% and 18.2%, respectively. Compared with LBWC, dietary supplementation with 1.0% l-arginine increased the serum insulin concentration (32.2%) and villus height in the jejunum (12.2%) and ileum (20.5%). In the jejunum, the mRNA levels for Claudin1 (105%) and glutathione peroxidase (36%) were higher, and the concentrations of IL-1 (31.7%) and tumor necrosis factor α (TNF-α) (30%) were lower in arginine-treated piglets than in the LBWC group. However, NO synthase activity and NO concentration in the jejunum of LBW piglets were not influenced by l-arginine supplementation. CONCLUSION: The results suggested that supplementation with 1.0% l-arginine not only promoted growth performance and improved intestinal functions in LBW piglets but also improved intestinal barrier functions and enhanced antioxidant capacity by an NO-independent pathway.
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spelling pubmed-62098072018-11-05 Dietary Arginine Supplementation Affects Intestinal Function by Enhancing Antioxidant Capacity of a Nitric Oxide–Independent Pathway in Low-Birth-Weight Piglets Zheng, Ping Song, Yi Tian, Yihang Zhang, Hao Yu, Bing He, Jun Mao, Xiangbing Yu, Jie Luo, Yuheng Luo, Junqiu Huang, Zhiqing Tian, Gang Chen, Hong Chen, Daiwen J Nutr Nutrient Physiology, Metabolism, and Nutrient-Nutrient Interactions BACKGROUND: Low-birth-weight (LBW) neonates are susceptible to intestinal dysfunction. Furthermore, the antioxidant capacity of LBW neonates is significantly lower compared with that of normal-birth-weight (NBW) neonates both at birth and at weaning. In LBW neonates, dietary supplementation with arginine has shown beneficial effects on intestinal function. OBJECTIVE: The present study explored the potential mechanisms of arginine-induced protective effects against intestinal dysfunction in LBW piglets. METHODS: Forty 4-d-old LBW piglets [body weight (BW): 1.05 ± 0.04 kg] (Large White × Landrace) were assigned to 4 treatments and artificially fed a whole-milk powder– and whey protein concentrate–based diet (containing 0.65% arginine) either not supplemented with arginine (LBWC) or supplemented with 0.5%, 1.0%, or 1.5% l-arginine for 21 d. In addition, 10 NBW siblings (BW: 1.96 ± 0.03 kg) were selected and fed the basal diet. Growth performance, intestinal morphology, mRNA expression of tight junction protein, redox-sensitive genes and nitric oxide (NO) synthase, cytokines, and redox indexes were determined. Data were subjected to 1-factor ANOVA. RESULTS: LBW piglets exhibited poorer growth performance (29.9%), lower Claudin1 mRNA level (63.6%), lower antioxidant capacity (22.9 ∼ 24.3%), and higher jejunum interleukin 1 (IL-1) concentration (18.8%) compared with NBW piglets. Dietary supplementation with 0.5% and 1.0% l-arginine significantly enhanced daily BW gain of LBW piglets by 13.6% and 18.2%, respectively. Compared with LBWC, dietary supplementation with 1.0% l-arginine increased the serum insulin concentration (32.2%) and villus height in the jejunum (12.2%) and ileum (20.5%). In the jejunum, the mRNA levels for Claudin1 (105%) and glutathione peroxidase (36%) were higher, and the concentrations of IL-1 (31.7%) and tumor necrosis factor α (TNF-α) (30%) were lower in arginine-treated piglets than in the LBWC group. However, NO synthase activity and NO concentration in the jejunum of LBW piglets were not influenced by l-arginine supplementation. CONCLUSION: The results suggested that supplementation with 1.0% l-arginine not only promoted growth performance and improved intestinal functions in LBW piglets but also improved intestinal barrier functions and enhanced antioxidant capacity by an NO-independent pathway. Oxford University Press 2018-11 2018-11-01 /pmc/articles/PMC6209807/ /pubmed/30383283 http://dx.doi.org/10.1093/jn/nxy198 Text en © 2018 American Society for Nutrition. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Nutrient Physiology, Metabolism, and Nutrient-Nutrient Interactions
Zheng, Ping
Song, Yi
Tian, Yihang
Zhang, Hao
Yu, Bing
He, Jun
Mao, Xiangbing
Yu, Jie
Luo, Yuheng
Luo, Junqiu
Huang, Zhiqing
Tian, Gang
Chen, Hong
Chen, Daiwen
Dietary Arginine Supplementation Affects Intestinal Function by Enhancing Antioxidant Capacity of a Nitric Oxide–Independent Pathway in Low-Birth-Weight Piglets
title Dietary Arginine Supplementation Affects Intestinal Function by Enhancing Antioxidant Capacity of a Nitric Oxide–Independent Pathway in Low-Birth-Weight Piglets
title_full Dietary Arginine Supplementation Affects Intestinal Function by Enhancing Antioxidant Capacity of a Nitric Oxide–Independent Pathway in Low-Birth-Weight Piglets
title_fullStr Dietary Arginine Supplementation Affects Intestinal Function by Enhancing Antioxidant Capacity of a Nitric Oxide–Independent Pathway in Low-Birth-Weight Piglets
title_full_unstemmed Dietary Arginine Supplementation Affects Intestinal Function by Enhancing Antioxidant Capacity of a Nitric Oxide–Independent Pathway in Low-Birth-Weight Piglets
title_short Dietary Arginine Supplementation Affects Intestinal Function by Enhancing Antioxidant Capacity of a Nitric Oxide–Independent Pathway in Low-Birth-Weight Piglets
title_sort dietary arginine supplementation affects intestinal function by enhancing antioxidant capacity of a nitric oxide–independent pathway in low-birth-weight piglets
topic Nutrient Physiology, Metabolism, and Nutrient-Nutrient Interactions
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6209807/
https://www.ncbi.nlm.nih.gov/pubmed/30383283
http://dx.doi.org/10.1093/jn/nxy198
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