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Nafion-coated cadmium pentacyanonitrosylferrate-modified glassy carbon electrode for detection of dopamine in biological samples

[Image: see text] Introduction: Dopamine is one of the key neurotransmitters (NTs) in nature, which plays a crucial role in the mammalian central nervous system (CNS). Its selective determination in the biological fluids is an essential need in the field of biomedicine studies. Methods: In this work...

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Autores principales: Johari-Ahar, Mohammad, Barar, Jaleh, Karami, Pari, Asgari, Davoud, Davaran, Soodabeh, Rashidi, Mohammad-Reza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tabriz University of Medical Sciences 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6209831/
https://www.ncbi.nlm.nih.gov/pubmed/30397581
http://dx.doi.org/10.15171/bi.2018.29
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author Johari-Ahar, Mohammad
Barar, Jaleh
Karami, Pari
Asgari, Davoud
Davaran, Soodabeh
Rashidi, Mohammad-Reza
author_facet Johari-Ahar, Mohammad
Barar, Jaleh
Karami, Pari
Asgari, Davoud
Davaran, Soodabeh
Rashidi, Mohammad-Reza
author_sort Johari-Ahar, Mohammad
collection PubMed
description [Image: see text] Introduction: Dopamine is one of the key neurotransmitters (NTs) in nature, which plays a crucial role in the mammalian central nervous system (CNS). Its selective determination in the biological fluids is an essential need in the field of biomedicine studies. Methods: In this work, an amperometric sensor was developed using Nafion-coated cadmium pentacyanonitrosylferrate (CdPCNF) modified glassy carbon (GC) electrode (Nafion|CdPCNF|GC electrode) as an electrocatalyst to detect dopamine (DA) in human serum samples. To develop this sensor, the surface of bare GC electrode was coated with the film of CdPCNF through an electropolymerization method and then the modified electrode was coated with Nafion to minimize interferences, especially those arising from the presence of anionic compounds. The electrocatalytic behavior of the modified electrodes was studied using the cyclic voltammetry and amperometry, and then the ability of the sensor for the determination of DA in synthetic and biological samples was investigated. Results: The modified electrode was showed a significant electrocatalytic activity for the oxidation of DA at pH 7.4. The limit of detection (LOD) was 0.7 µM and also no interference effects arose from ascorbic acid (AA), uric acid (UA) or the other biological NTs was observed in the DA detection using the modified Nafion|CdPCNF|GC electrode. Conclusion: In comparison with the bare electrode, the Nafion|CdPCNF|GC electrode could determine DA in the biological samples with adequate sensitivity and selectivity. Therefore, we propose that the modified electrode is utilizable as an amperometric DA sensor for the biological sample analysis.
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spelling pubmed-62098312018-11-05 Nafion-coated cadmium pentacyanonitrosylferrate-modified glassy carbon electrode for detection of dopamine in biological samples Johari-Ahar, Mohammad Barar, Jaleh Karami, Pari Asgari, Davoud Davaran, Soodabeh Rashidi, Mohammad-Reza Bioimpacts Original Research [Image: see text] Introduction: Dopamine is one of the key neurotransmitters (NTs) in nature, which plays a crucial role in the mammalian central nervous system (CNS). Its selective determination in the biological fluids is an essential need in the field of biomedicine studies. Methods: In this work, an amperometric sensor was developed using Nafion-coated cadmium pentacyanonitrosylferrate (CdPCNF) modified glassy carbon (GC) electrode (Nafion|CdPCNF|GC electrode) as an electrocatalyst to detect dopamine (DA) in human serum samples. To develop this sensor, the surface of bare GC electrode was coated with the film of CdPCNF through an electropolymerization method and then the modified electrode was coated with Nafion to minimize interferences, especially those arising from the presence of anionic compounds. The electrocatalytic behavior of the modified electrodes was studied using the cyclic voltammetry and amperometry, and then the ability of the sensor for the determination of DA in synthetic and biological samples was investigated. Results: The modified electrode was showed a significant electrocatalytic activity for the oxidation of DA at pH 7.4. The limit of detection (LOD) was 0.7 µM and also no interference effects arose from ascorbic acid (AA), uric acid (UA) or the other biological NTs was observed in the DA detection using the modified Nafion|CdPCNF|GC electrode. Conclusion: In comparison with the bare electrode, the Nafion|CdPCNF|GC electrode could determine DA in the biological samples with adequate sensitivity and selectivity. Therefore, we propose that the modified electrode is utilizable as an amperometric DA sensor for the biological sample analysis. Tabriz University of Medical Sciences 2018 2017-08-30 /pmc/articles/PMC6209831/ /pubmed/30397581 http://dx.doi.org/10.15171/bi.2018.29 Text en © 2018 The Author(s) This work is published by BioImpacts as an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/). Non-commercial uses of the work are permitted, provided the original work is properly cited.
spellingShingle Original Research
Johari-Ahar, Mohammad
Barar, Jaleh
Karami, Pari
Asgari, Davoud
Davaran, Soodabeh
Rashidi, Mohammad-Reza
Nafion-coated cadmium pentacyanonitrosylferrate-modified glassy carbon electrode for detection of dopamine in biological samples
title Nafion-coated cadmium pentacyanonitrosylferrate-modified glassy carbon electrode for detection of dopamine in biological samples
title_full Nafion-coated cadmium pentacyanonitrosylferrate-modified glassy carbon electrode for detection of dopamine in biological samples
title_fullStr Nafion-coated cadmium pentacyanonitrosylferrate-modified glassy carbon electrode for detection of dopamine in biological samples
title_full_unstemmed Nafion-coated cadmium pentacyanonitrosylferrate-modified glassy carbon electrode for detection of dopamine in biological samples
title_short Nafion-coated cadmium pentacyanonitrosylferrate-modified glassy carbon electrode for detection of dopamine in biological samples
title_sort nafion-coated cadmium pentacyanonitrosylferrate-modified glassy carbon electrode for detection of dopamine in biological samples
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6209831/
https://www.ncbi.nlm.nih.gov/pubmed/30397581
http://dx.doi.org/10.15171/bi.2018.29
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