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Blocking-Free ELISA Using a Gold Nanoparticle Layer Coated Commercial Microwell Plate

Enzyme-linked immunosorbent assays (ELISA) show extensive application in immunoassays, to detect and monitor protein biomarkers in clinical diagnosis. Nevertheless, the time required and its multiple steps limit its application. We take advantage of a polyethyleneimine (PEI) gold nanoparticle (GNP)...

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Autores principales: Huang, Ruijia, Zhang, Ke, Zhu, Guoshuai, Sun, Zhencheng, He, Songliang, Chen, Wenwen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6210089/
https://www.ncbi.nlm.nih.gov/pubmed/30347684
http://dx.doi.org/10.3390/s18103537
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author Huang, Ruijia
Zhang, Ke
Zhu, Guoshuai
Sun, Zhencheng
He, Songliang
Chen, Wenwen
author_facet Huang, Ruijia
Zhang, Ke
Zhu, Guoshuai
Sun, Zhencheng
He, Songliang
Chen, Wenwen
author_sort Huang, Ruijia
collection PubMed
description Enzyme-linked immunosorbent assays (ELISA) show extensive application in immunoassays, to detect and monitor protein biomarkers in clinical diagnosis. Nevertheless, the time required and its multiple steps limit its application. We take advantage of a polyethyleneimine (PEI) gold nanoparticle (GNP) coated microwell plate to perform blocking-free ELISA, in which no nonspecific protein adsorption appears on the GNP layer. If the PEI-GNP coated microwell plate and immobilization of captured antibodies on the plate are prepared in advance, such as using an ELISA kit, the whole ELISA process can be finished in less than 2 h. Meanwhile, we have ensured that the GNP layer can preserve the precision and good linearity of ELISA without causing negative effects on the plate.
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spelling pubmed-62100892018-11-02 Blocking-Free ELISA Using a Gold Nanoparticle Layer Coated Commercial Microwell Plate Huang, Ruijia Zhang, Ke Zhu, Guoshuai Sun, Zhencheng He, Songliang Chen, Wenwen Sensors (Basel) Article Enzyme-linked immunosorbent assays (ELISA) show extensive application in immunoassays, to detect and monitor protein biomarkers in clinical diagnosis. Nevertheless, the time required and its multiple steps limit its application. We take advantage of a polyethyleneimine (PEI) gold nanoparticle (GNP) coated microwell plate to perform blocking-free ELISA, in which no nonspecific protein adsorption appears on the GNP layer. If the PEI-GNP coated microwell plate and immobilization of captured antibodies on the plate are prepared in advance, such as using an ELISA kit, the whole ELISA process can be finished in less than 2 h. Meanwhile, we have ensured that the GNP layer can preserve the precision and good linearity of ELISA without causing negative effects on the plate. MDPI 2018-10-19 /pmc/articles/PMC6210089/ /pubmed/30347684 http://dx.doi.org/10.3390/s18103537 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Huang, Ruijia
Zhang, Ke
Zhu, Guoshuai
Sun, Zhencheng
He, Songliang
Chen, Wenwen
Blocking-Free ELISA Using a Gold Nanoparticle Layer Coated Commercial Microwell Plate
title Blocking-Free ELISA Using a Gold Nanoparticle Layer Coated Commercial Microwell Plate
title_full Blocking-Free ELISA Using a Gold Nanoparticle Layer Coated Commercial Microwell Plate
title_fullStr Blocking-Free ELISA Using a Gold Nanoparticle Layer Coated Commercial Microwell Plate
title_full_unstemmed Blocking-Free ELISA Using a Gold Nanoparticle Layer Coated Commercial Microwell Plate
title_short Blocking-Free ELISA Using a Gold Nanoparticle Layer Coated Commercial Microwell Plate
title_sort blocking-free elisa using a gold nanoparticle layer coated commercial microwell plate
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6210089/
https://www.ncbi.nlm.nih.gov/pubmed/30347684
http://dx.doi.org/10.3390/s18103537
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