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Blocking-Free ELISA Using a Gold Nanoparticle Layer Coated Commercial Microwell Plate
Enzyme-linked immunosorbent assays (ELISA) show extensive application in immunoassays, to detect and monitor protein biomarkers in clinical diagnosis. Nevertheless, the time required and its multiple steps limit its application. We take advantage of a polyethyleneimine (PEI) gold nanoparticle (GNP)...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6210089/ https://www.ncbi.nlm.nih.gov/pubmed/30347684 http://dx.doi.org/10.3390/s18103537 |
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author | Huang, Ruijia Zhang, Ke Zhu, Guoshuai Sun, Zhencheng He, Songliang Chen, Wenwen |
author_facet | Huang, Ruijia Zhang, Ke Zhu, Guoshuai Sun, Zhencheng He, Songliang Chen, Wenwen |
author_sort | Huang, Ruijia |
collection | PubMed |
description | Enzyme-linked immunosorbent assays (ELISA) show extensive application in immunoassays, to detect and monitor protein biomarkers in clinical diagnosis. Nevertheless, the time required and its multiple steps limit its application. We take advantage of a polyethyleneimine (PEI) gold nanoparticle (GNP) coated microwell plate to perform blocking-free ELISA, in which no nonspecific protein adsorption appears on the GNP layer. If the PEI-GNP coated microwell plate and immobilization of captured antibodies on the plate are prepared in advance, such as using an ELISA kit, the whole ELISA process can be finished in less than 2 h. Meanwhile, we have ensured that the GNP layer can preserve the precision and good linearity of ELISA without causing negative effects on the plate. |
format | Online Article Text |
id | pubmed-6210089 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62100892018-11-02 Blocking-Free ELISA Using a Gold Nanoparticle Layer Coated Commercial Microwell Plate Huang, Ruijia Zhang, Ke Zhu, Guoshuai Sun, Zhencheng He, Songliang Chen, Wenwen Sensors (Basel) Article Enzyme-linked immunosorbent assays (ELISA) show extensive application in immunoassays, to detect and monitor protein biomarkers in clinical diagnosis. Nevertheless, the time required and its multiple steps limit its application. We take advantage of a polyethyleneimine (PEI) gold nanoparticle (GNP) coated microwell plate to perform blocking-free ELISA, in which no nonspecific protein adsorption appears on the GNP layer. If the PEI-GNP coated microwell plate and immobilization of captured antibodies on the plate are prepared in advance, such as using an ELISA kit, the whole ELISA process can be finished in less than 2 h. Meanwhile, we have ensured that the GNP layer can preserve the precision and good linearity of ELISA without causing negative effects on the plate. MDPI 2018-10-19 /pmc/articles/PMC6210089/ /pubmed/30347684 http://dx.doi.org/10.3390/s18103537 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Huang, Ruijia Zhang, Ke Zhu, Guoshuai Sun, Zhencheng He, Songliang Chen, Wenwen Blocking-Free ELISA Using a Gold Nanoparticle Layer Coated Commercial Microwell Plate |
title | Blocking-Free ELISA Using a Gold Nanoparticle Layer Coated Commercial Microwell Plate |
title_full | Blocking-Free ELISA Using a Gold Nanoparticle Layer Coated Commercial Microwell Plate |
title_fullStr | Blocking-Free ELISA Using a Gold Nanoparticle Layer Coated Commercial Microwell Plate |
title_full_unstemmed | Blocking-Free ELISA Using a Gold Nanoparticle Layer Coated Commercial Microwell Plate |
title_short | Blocking-Free ELISA Using a Gold Nanoparticle Layer Coated Commercial Microwell Plate |
title_sort | blocking-free elisa using a gold nanoparticle layer coated commercial microwell plate |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6210089/ https://www.ncbi.nlm.nih.gov/pubmed/30347684 http://dx.doi.org/10.3390/s18103537 |
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