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A Label-Free Fluorescent AND Logic Gate Aptasensor for Sensitive ATP Detection
In this study, a label-free fluorescent, enzyme-free, simple, highly sensitive AND logic gate aptasensor was developed for the detection of adenosine triphosphate (ATP). Double-stranded deoxyribonucleic acid (DNA) with cohesive ends was attached to graphene oxide (GO) to form an aptasensor probe. AT...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6210427/ https://www.ncbi.nlm.nih.gov/pubmed/30274300 http://dx.doi.org/10.3390/s18103281 |
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author | Zhang, Jingjing Yang, Chunzheng Niu, Chaoqun Liu, Chen Cai, Xuepin Du, Jie Chen, Yong |
author_facet | Zhang, Jingjing Yang, Chunzheng Niu, Chaoqun Liu, Chen Cai, Xuepin Du, Jie Chen, Yong |
author_sort | Zhang, Jingjing |
collection | PubMed |
description | In this study, a label-free fluorescent, enzyme-free, simple, highly sensitive AND logic gate aptasensor was developed for the detection of adenosine triphosphate (ATP). Double-stranded deoxyribonucleic acid (DNA) with cohesive ends was attached to graphene oxide (GO) to form an aptasensor probe. ATP and single-stranded DNA were used as input signals. Fluorescence intensity of PicoGreen dye was used as an output signal. The biosensor-related performances, including the logic gate construction, reaction time, linearity, sensitivity, and specificity, were investigated and the results showed that an AND logic gate was successfully constructed. The ATP detection range was found to be 20 to 400 nM (R(2) = 0.9943) with limit of detection (LOD) of 142.6 pM, and the sensitivity range was 1.846 × 10(6) to 2.988 × 10(6) M(−1). This method for the detection of ATP has the characteristics of being simple, low cost, and highly sensitive. |
format | Online Article Text |
id | pubmed-6210427 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62104272018-11-02 A Label-Free Fluorescent AND Logic Gate Aptasensor for Sensitive ATP Detection Zhang, Jingjing Yang, Chunzheng Niu, Chaoqun Liu, Chen Cai, Xuepin Du, Jie Chen, Yong Sensors (Basel) Article In this study, a label-free fluorescent, enzyme-free, simple, highly sensitive AND logic gate aptasensor was developed for the detection of adenosine triphosphate (ATP). Double-stranded deoxyribonucleic acid (DNA) with cohesive ends was attached to graphene oxide (GO) to form an aptasensor probe. ATP and single-stranded DNA were used as input signals. Fluorescence intensity of PicoGreen dye was used as an output signal. The biosensor-related performances, including the logic gate construction, reaction time, linearity, sensitivity, and specificity, were investigated and the results showed that an AND logic gate was successfully constructed. The ATP detection range was found to be 20 to 400 nM (R(2) = 0.9943) with limit of detection (LOD) of 142.6 pM, and the sensitivity range was 1.846 × 10(6) to 2.988 × 10(6) M(−1). This method for the detection of ATP has the characteristics of being simple, low cost, and highly sensitive. MDPI 2018-09-29 /pmc/articles/PMC6210427/ /pubmed/30274300 http://dx.doi.org/10.3390/s18103281 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhang, Jingjing Yang, Chunzheng Niu, Chaoqun Liu, Chen Cai, Xuepin Du, Jie Chen, Yong A Label-Free Fluorescent AND Logic Gate Aptasensor for Sensitive ATP Detection |
title | A Label-Free Fluorescent AND Logic Gate Aptasensor for Sensitive ATP Detection |
title_full | A Label-Free Fluorescent AND Logic Gate Aptasensor for Sensitive ATP Detection |
title_fullStr | A Label-Free Fluorescent AND Logic Gate Aptasensor for Sensitive ATP Detection |
title_full_unstemmed | A Label-Free Fluorescent AND Logic Gate Aptasensor for Sensitive ATP Detection |
title_short | A Label-Free Fluorescent AND Logic Gate Aptasensor for Sensitive ATP Detection |
title_sort | label-free fluorescent and logic gate aptasensor for sensitive atp detection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6210427/ https://www.ncbi.nlm.nih.gov/pubmed/30274300 http://dx.doi.org/10.3390/s18103281 |
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