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Comparative analysis between slow freezing and ultra-rapid freezing for human sperm cryopreservation

OBJECTIVE: Cryopreservation of human spermatozoa is fundamental in assisted reproductive technology. At present, slow freezing techniques are widely used for sperm cryopreservation. Recently, sperm vitrification has been proposed as an alternative to slow freezing. This study aimed to compare the ef...

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Autores principales: S. Riva, Natalí, Ruhlmann, Claudio, S. Iaizzo, Rocío, López, Carla A Marcial, Martínez, Alejandro Gustavo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Brazilian Society of Assisted Reproduction 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6210620/
https://www.ncbi.nlm.nih.gov/pubmed/30132630
http://dx.doi.org/10.5935/1518-0557.20180060
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author S. Riva, Natalí
Ruhlmann, Claudio
S. Iaizzo, Rocío
López, Carla A Marcial
Martínez, Alejandro Gustavo
author_facet S. Riva, Natalí
Ruhlmann, Claudio
S. Iaizzo, Rocío
López, Carla A Marcial
Martínez, Alejandro Gustavo
author_sort S. Riva, Natalí
collection PubMed
description OBJECTIVE: Cryopreservation of human spermatozoa is fundamental in assisted reproductive technology. At present, slow freezing techniques are widely used for sperm cryopreservation. Recently, sperm vitrification has been proposed as an alternative to slow freezing. This study aimed to compare the efficiency of slow versus ultra-rapid freezing after thawing and to determine the level of DNA fragmentation in post-thaw normal human semen samples processed through each of the cryopreservation techniques. METHODS: Ultra-rapid freezing is a method that only differs from conventional ultra-rapid freezing in the use of sucrose as a cryoprotectant. In experiment 1, 24 semen samples were used to compare sperm recovery rates after slow and ultra-rapid sperm freezing. In experiment 2, 18 semen samples were used to compare post-thaw sperm DNA fragmentation levels after each of the cryopreservation techniques. RESULTS: In experiment 1, no significant differences were observed in sperm concentration recovery rates, although slow freezing showed a lower progressive motility rate than ultra-rapid freezing (16.6±7.4% vs. 34.7±10.2%), and higher non-progressive and immotile sperm counts (9.0±4.0% vs. 7.6±2.8%; and 74.4±10.1% vs. 57.8±10.3%, respectively). In experiment 2, sperm DNA fragmentation after thawing was significantly higher in slow freezing than in fresh post gradient processing and ultra-rapid freezing samples (47.3±13.4% vs. 9.1±3.7% vs. 14.6±4.6%, respectively). CONCLUSION: Sperm ultra-rapid freezing may be an alternative to slow freezing with better recovery results and less apparent DNA damage.
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spelling pubmed-62106202018-11-13 Comparative analysis between slow freezing and ultra-rapid freezing for human sperm cryopreservation S. Riva, Natalí Ruhlmann, Claudio S. Iaizzo, Rocío López, Carla A Marcial Martínez, Alejandro Gustavo JBRA Assist Reprod Original Article OBJECTIVE: Cryopreservation of human spermatozoa is fundamental in assisted reproductive technology. At present, slow freezing techniques are widely used for sperm cryopreservation. Recently, sperm vitrification has been proposed as an alternative to slow freezing. This study aimed to compare the efficiency of slow versus ultra-rapid freezing after thawing and to determine the level of DNA fragmentation in post-thaw normal human semen samples processed through each of the cryopreservation techniques. METHODS: Ultra-rapid freezing is a method that only differs from conventional ultra-rapid freezing in the use of sucrose as a cryoprotectant. In experiment 1, 24 semen samples were used to compare sperm recovery rates after slow and ultra-rapid sperm freezing. In experiment 2, 18 semen samples were used to compare post-thaw sperm DNA fragmentation levels after each of the cryopreservation techniques. RESULTS: In experiment 1, no significant differences were observed in sperm concentration recovery rates, although slow freezing showed a lower progressive motility rate than ultra-rapid freezing (16.6±7.4% vs. 34.7±10.2%), and higher non-progressive and immotile sperm counts (9.0±4.0% vs. 7.6±2.8%; and 74.4±10.1% vs. 57.8±10.3%, respectively). In experiment 2, sperm DNA fragmentation after thawing was significantly higher in slow freezing than in fresh post gradient processing and ultra-rapid freezing samples (47.3±13.4% vs. 9.1±3.7% vs. 14.6±4.6%, respectively). CONCLUSION: Sperm ultra-rapid freezing may be an alternative to slow freezing with better recovery results and less apparent DNA damage. Brazilian Society of Assisted Reproduction 2018 /pmc/articles/PMC6210620/ /pubmed/30132630 http://dx.doi.org/10.5935/1518-0557.20180060 Text en http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
S. Riva, Natalí
Ruhlmann, Claudio
S. Iaizzo, Rocío
López, Carla A Marcial
Martínez, Alejandro Gustavo
Comparative analysis between slow freezing and ultra-rapid freezing for human sperm cryopreservation
title Comparative analysis between slow freezing and ultra-rapid freezing for human sperm cryopreservation
title_full Comparative analysis between slow freezing and ultra-rapid freezing for human sperm cryopreservation
title_fullStr Comparative analysis between slow freezing and ultra-rapid freezing for human sperm cryopreservation
title_full_unstemmed Comparative analysis between slow freezing and ultra-rapid freezing for human sperm cryopreservation
title_short Comparative analysis between slow freezing and ultra-rapid freezing for human sperm cryopreservation
title_sort comparative analysis between slow freezing and ultra-rapid freezing for human sperm cryopreservation
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6210620/
https://www.ncbi.nlm.nih.gov/pubmed/30132630
http://dx.doi.org/10.5935/1518-0557.20180060
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