Cargando…
The Effect of Ethanol on Telomere Dynamics and Regulation in Human Cells
Telomeres (TLs) protect chromosome ends from chromosomal fusion and degradation, thus conferring genomic stability, and play crucial roles in cellular aging and disease. Recent studies have found a correlation between environmental, physiological and even mental stresses on TL dynamics in humans. Ho...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6210749/ https://www.ncbi.nlm.nih.gov/pubmed/30326633 http://dx.doi.org/10.3390/cells7100169 |
_version_ | 1783367188636237824 |
---|---|
author | Harpaz, Tomer Abumock, Heba Beery, Einat Edel, Yonatan Lahav, Meir Rozovski, Uri Uziel, Orit |
author_facet | Harpaz, Tomer Abumock, Heba Beery, Einat Edel, Yonatan Lahav, Meir Rozovski, Uri Uziel, Orit |
author_sort | Harpaz, Tomer |
collection | PubMed |
description | Telomeres (TLs) protect chromosome ends from chromosomal fusion and degradation, thus conferring genomic stability, and play crucial roles in cellular aging and disease. Recent studies have found a correlation between environmental, physiological and even mental stresses on TL dynamics in humans. However, the causal relationship between stress and TL length and the molecular mechanisms underlying that relationship are far from being understood. This study describes the effect of moderate concentrations of ethanol, equivalent to social drinking, on human TL dynamics and partially elucidates the mechanism mediating this effect. The exposure of Immortalized human foreskin fibroblast, primary human foreskin fibroblast and human hepatocellular carcinoma cells to 25 mM ethanol for one week moderately shortened telomeres in all cells. Similar TL shortening was obtained following cells’ exposure to 25 µM acetaldehyde (AcH) and to a much lower extent after exposure to 4-methylpyrazolean, an inhibitor of alcoholdehydrogenase, suggesting that AcH plays a key role in ethanol-dependent telomere shortening. Telomerase activity was not involved in this effect. TRF2 and several TRF2 binding proteins increased their binding to TLs after ethanol treatment, implying their involvement in this effect. The methylation status of several sub-telomeric regions increased in response to EtOH exposure. Gene expression profiling showed distinct patterns in cells treated with EtOH and in cells recovered from EtOH. In addition to cellular ageing, the described telomere shortening may contribute to the carcinogenic potential of acute alcohol consumption; both are associated with the shortening of TLs and provide new insights regarding the moderate consumption of alcohol referred to as “social drinking.” |
format | Online Article Text |
id | pubmed-6210749 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62107492018-11-02 The Effect of Ethanol on Telomere Dynamics and Regulation in Human Cells Harpaz, Tomer Abumock, Heba Beery, Einat Edel, Yonatan Lahav, Meir Rozovski, Uri Uziel, Orit Cells Article Telomeres (TLs) protect chromosome ends from chromosomal fusion and degradation, thus conferring genomic stability, and play crucial roles in cellular aging and disease. Recent studies have found a correlation between environmental, physiological and even mental stresses on TL dynamics in humans. However, the causal relationship between stress and TL length and the molecular mechanisms underlying that relationship are far from being understood. This study describes the effect of moderate concentrations of ethanol, equivalent to social drinking, on human TL dynamics and partially elucidates the mechanism mediating this effect. The exposure of Immortalized human foreskin fibroblast, primary human foreskin fibroblast and human hepatocellular carcinoma cells to 25 mM ethanol for one week moderately shortened telomeres in all cells. Similar TL shortening was obtained following cells’ exposure to 25 µM acetaldehyde (AcH) and to a much lower extent after exposure to 4-methylpyrazolean, an inhibitor of alcoholdehydrogenase, suggesting that AcH plays a key role in ethanol-dependent telomere shortening. Telomerase activity was not involved in this effect. TRF2 and several TRF2 binding proteins increased their binding to TLs after ethanol treatment, implying their involvement in this effect. The methylation status of several sub-telomeric regions increased in response to EtOH exposure. Gene expression profiling showed distinct patterns in cells treated with EtOH and in cells recovered from EtOH. In addition to cellular ageing, the described telomere shortening may contribute to the carcinogenic potential of acute alcohol consumption; both are associated with the shortening of TLs and provide new insights regarding the moderate consumption of alcohol referred to as “social drinking.” MDPI 2018-10-15 /pmc/articles/PMC6210749/ /pubmed/30326633 http://dx.doi.org/10.3390/cells7100169 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Harpaz, Tomer Abumock, Heba Beery, Einat Edel, Yonatan Lahav, Meir Rozovski, Uri Uziel, Orit The Effect of Ethanol on Telomere Dynamics and Regulation in Human Cells |
title | The Effect of Ethanol on Telomere Dynamics and Regulation in Human Cells |
title_full | The Effect of Ethanol on Telomere Dynamics and Regulation in Human Cells |
title_fullStr | The Effect of Ethanol on Telomere Dynamics and Regulation in Human Cells |
title_full_unstemmed | The Effect of Ethanol on Telomere Dynamics and Regulation in Human Cells |
title_short | The Effect of Ethanol on Telomere Dynamics and Regulation in Human Cells |
title_sort | effect of ethanol on telomere dynamics and regulation in human cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6210749/ https://www.ncbi.nlm.nih.gov/pubmed/30326633 http://dx.doi.org/10.3390/cells7100169 |
work_keys_str_mv | AT harpaztomer theeffectofethanolontelomeredynamicsandregulationinhumancells AT abumockheba theeffectofethanolontelomeredynamicsandregulationinhumancells AT beeryeinat theeffectofethanolontelomeredynamicsandregulationinhumancells AT edelyonatan theeffectofethanolontelomeredynamicsandregulationinhumancells AT lahavmeir theeffectofethanolontelomeredynamicsandregulationinhumancells AT rozovskiuri theeffectofethanolontelomeredynamicsandregulationinhumancells AT uzielorit theeffectofethanolontelomeredynamicsandregulationinhumancells AT harpaztomer effectofethanolontelomeredynamicsandregulationinhumancells AT abumockheba effectofethanolontelomeredynamicsandregulationinhumancells AT beeryeinat effectofethanolontelomeredynamicsandregulationinhumancells AT edelyonatan effectofethanolontelomeredynamicsandregulationinhumancells AT lahavmeir effectofethanolontelomeredynamicsandregulationinhumancells AT rozovskiuri effectofethanolontelomeredynamicsandregulationinhumancells AT uzielorit effectofethanolontelomeredynamicsandregulationinhumancells |