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Cytomorphological Differences between Liquid-Based Cytology and Conventional Smears in Fine-Needle Aspirates of Thyroid Lesions

BACKGROUND: Liquid-based cytology (LBC) is becoming an independent processing modality for cytology specimens. Decreased obscuration, single-slide examination, lesser screening time, and potential application of ancillary techniques are the various advantages it offers. AIM: To study and compare the...

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Autores principales: Sharma, Shuchita, Agarwal, Shilpi, Jain, Manjula, Singh, Gautam Bir, Andley, Manoj
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6210815/
https://www.ncbi.nlm.nih.gov/pubmed/30498291
http://dx.doi.org/10.4103/JOC.JOC_150_17
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author Sharma, Shuchita
Agarwal, Shilpi
Jain, Manjula
Singh, Gautam Bir
Andley, Manoj
author_facet Sharma, Shuchita
Agarwal, Shilpi
Jain, Manjula
Singh, Gautam Bir
Andley, Manoj
author_sort Sharma, Shuchita
collection PubMed
description BACKGROUND: Liquid-based cytology (LBC) is becoming an independent processing modality for cytology specimens. Decreased obscuration, single-slide examination, lesser screening time, and potential application of ancillary techniques are the various advantages it offers. AIM: To study and compare the cytological features of fine-needle aspirates from thyroid swellings on LBC with conventional smears (CS). MATERIALS AND METHODS: Fine-needle aspiration was performed on 150 patients with thyroid nodule. The aspirate was first used to prepare a minimum of two CS and the remaining aspirate was used to prepare one SurePath smear. The final diagnosis was given according to the Bethesda system for reporting thyroid cytopathology (2007). Cytomorphological parameters were semi-quantitatively scored. Pearson's Chi-square test was used and P value was calculated. A P value <0.05 was considered significant. Cytohistological correlation was done wherever possible. RESULTS: LBC showed higher nondiagnostic rate than CS. Significant cytomorphological differences on LBC included: (i) higher frequency of single, naked nuclei; (ii) lesser nuclear and cytoplasmic details; (iii) decreased colloid which appeared differently (as napkin fold and dense droplet); (iv) increased cyst macrophages; and (v) decreased obscuration by blood. CONCLUSION: LBC can supplement CS but cannot replace it.
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spelling pubmed-62108152018-11-29 Cytomorphological Differences between Liquid-Based Cytology and Conventional Smears in Fine-Needle Aspirates of Thyroid Lesions Sharma, Shuchita Agarwal, Shilpi Jain, Manjula Singh, Gautam Bir Andley, Manoj J Cytol Original Article BACKGROUND: Liquid-based cytology (LBC) is becoming an independent processing modality for cytology specimens. Decreased obscuration, single-slide examination, lesser screening time, and potential application of ancillary techniques are the various advantages it offers. AIM: To study and compare the cytological features of fine-needle aspirates from thyroid swellings on LBC with conventional smears (CS). MATERIALS AND METHODS: Fine-needle aspiration was performed on 150 patients with thyroid nodule. The aspirate was first used to prepare a minimum of two CS and the remaining aspirate was used to prepare one SurePath smear. The final diagnosis was given according to the Bethesda system for reporting thyroid cytopathology (2007). Cytomorphological parameters were semi-quantitatively scored. Pearson's Chi-square test was used and P value was calculated. A P value <0.05 was considered significant. Cytohistological correlation was done wherever possible. RESULTS: LBC showed higher nondiagnostic rate than CS. Significant cytomorphological differences on LBC included: (i) higher frequency of single, naked nuclei; (ii) lesser nuclear and cytoplasmic details; (iii) decreased colloid which appeared differently (as napkin fold and dense droplet); (iv) increased cyst macrophages; and (v) decreased obscuration by blood. CONCLUSION: LBC can supplement CS but cannot replace it. Medknow Publications & Media Pvt Ltd 2018 /pmc/articles/PMC6210815/ /pubmed/30498291 http://dx.doi.org/10.4103/JOC.JOC_150_17 Text en Copyright: © 2018 Journal of Cytology http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Sharma, Shuchita
Agarwal, Shilpi
Jain, Manjula
Singh, Gautam Bir
Andley, Manoj
Cytomorphological Differences between Liquid-Based Cytology and Conventional Smears in Fine-Needle Aspirates of Thyroid Lesions
title Cytomorphological Differences between Liquid-Based Cytology and Conventional Smears in Fine-Needle Aspirates of Thyroid Lesions
title_full Cytomorphological Differences between Liquid-Based Cytology and Conventional Smears in Fine-Needle Aspirates of Thyroid Lesions
title_fullStr Cytomorphological Differences between Liquid-Based Cytology and Conventional Smears in Fine-Needle Aspirates of Thyroid Lesions
title_full_unstemmed Cytomorphological Differences between Liquid-Based Cytology and Conventional Smears in Fine-Needle Aspirates of Thyroid Lesions
title_short Cytomorphological Differences between Liquid-Based Cytology and Conventional Smears in Fine-Needle Aspirates of Thyroid Lesions
title_sort cytomorphological differences between liquid-based cytology and conventional smears in fine-needle aspirates of thyroid lesions
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6210815/
https://www.ncbi.nlm.nih.gov/pubmed/30498291
http://dx.doi.org/10.4103/JOC.JOC_150_17
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