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Non-Linear Cellular Dielectrophoretic Behavior Characterization Using Dielectrophoretic Tweezers-Based Force Spectroscopy inside a Microfluidic Device

Characterization of cellular dielectrophoretic (DEP) behaviors, when cells are exposed to an alternating current (AC) electric field of varying frequency, is fundamentally important to many applications using dielectrophoresis. However, to date, that characterization has been performed with monotoni...

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Autores principales: Choi, Seungyeop, Ko, Kwanhwi, Lim, Jongwon, Kim, Sung Hoon, Woo, Sung-Hun, Kim, Yoon Suk, Key, Jaehong, Lee, Sei Young, Park, In Su, Lee, Sang Woo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6210972/
https://www.ncbi.nlm.nih.gov/pubmed/30347732
http://dx.doi.org/10.3390/s18103543
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author Choi, Seungyeop
Ko, Kwanhwi
Lim, Jongwon
Kim, Sung Hoon
Woo, Sung-Hun
Kim, Yoon Suk
Key, Jaehong
Lee, Sei Young
Park, In Su
Lee, Sang Woo
author_facet Choi, Seungyeop
Ko, Kwanhwi
Lim, Jongwon
Kim, Sung Hoon
Woo, Sung-Hun
Kim, Yoon Suk
Key, Jaehong
Lee, Sei Young
Park, In Su
Lee, Sang Woo
author_sort Choi, Seungyeop
collection PubMed
description Characterization of cellular dielectrophoretic (DEP) behaviors, when cells are exposed to an alternating current (AC) electric field of varying frequency, is fundamentally important to many applications using dielectrophoresis. However, to date, that characterization has been performed with monotonically increasing or decreasing frequency, not with successive increases and decreases, even though cells might behave differently with those frequency modulations due to the nonlinear cellular electrodynamic responses reported in previous works. In this report, we present a method to trace the behaviors of numerous cells simultaneously at the single-cell level in a simple, robust manner using dielectrophoretic tweezers-based force spectroscopy. Using this method, the behaviors of more than 150 cells were traced in a single environment at the same time, while a modulated DEP force acted upon them, resulting in characterization of nonlinear DEP cellular behaviors and generation of different cross-over frequencies in living cells by modulating the DEP force. This study demonstrated that living cells can have non-linear di-polarized responses depending on the modulation direction of the applied frequency as well as providing a simple and reliable platform from which to measure a cellular cross-over frequency and characterize its nonlinear property.
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spelling pubmed-62109722018-11-02 Non-Linear Cellular Dielectrophoretic Behavior Characterization Using Dielectrophoretic Tweezers-Based Force Spectroscopy inside a Microfluidic Device Choi, Seungyeop Ko, Kwanhwi Lim, Jongwon Kim, Sung Hoon Woo, Sung-Hun Kim, Yoon Suk Key, Jaehong Lee, Sei Young Park, In Su Lee, Sang Woo Sensors (Basel) Article Characterization of cellular dielectrophoretic (DEP) behaviors, when cells are exposed to an alternating current (AC) electric field of varying frequency, is fundamentally important to many applications using dielectrophoresis. However, to date, that characterization has been performed with monotonically increasing or decreasing frequency, not with successive increases and decreases, even though cells might behave differently with those frequency modulations due to the nonlinear cellular electrodynamic responses reported in previous works. In this report, we present a method to trace the behaviors of numerous cells simultaneously at the single-cell level in a simple, robust manner using dielectrophoretic tweezers-based force spectroscopy. Using this method, the behaviors of more than 150 cells were traced in a single environment at the same time, while a modulated DEP force acted upon them, resulting in characterization of nonlinear DEP cellular behaviors and generation of different cross-over frequencies in living cells by modulating the DEP force. This study demonstrated that living cells can have non-linear di-polarized responses depending on the modulation direction of the applied frequency as well as providing a simple and reliable platform from which to measure a cellular cross-over frequency and characterize its nonlinear property. MDPI 2018-10-19 /pmc/articles/PMC6210972/ /pubmed/30347732 http://dx.doi.org/10.3390/s18103543 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Choi, Seungyeop
Ko, Kwanhwi
Lim, Jongwon
Kim, Sung Hoon
Woo, Sung-Hun
Kim, Yoon Suk
Key, Jaehong
Lee, Sei Young
Park, In Su
Lee, Sang Woo
Non-Linear Cellular Dielectrophoretic Behavior Characterization Using Dielectrophoretic Tweezers-Based Force Spectroscopy inside a Microfluidic Device
title Non-Linear Cellular Dielectrophoretic Behavior Characterization Using Dielectrophoretic Tweezers-Based Force Spectroscopy inside a Microfluidic Device
title_full Non-Linear Cellular Dielectrophoretic Behavior Characterization Using Dielectrophoretic Tweezers-Based Force Spectroscopy inside a Microfluidic Device
title_fullStr Non-Linear Cellular Dielectrophoretic Behavior Characterization Using Dielectrophoretic Tweezers-Based Force Spectroscopy inside a Microfluidic Device
title_full_unstemmed Non-Linear Cellular Dielectrophoretic Behavior Characterization Using Dielectrophoretic Tweezers-Based Force Spectroscopy inside a Microfluidic Device
title_short Non-Linear Cellular Dielectrophoretic Behavior Characterization Using Dielectrophoretic Tweezers-Based Force Spectroscopy inside a Microfluidic Device
title_sort non-linear cellular dielectrophoretic behavior characterization using dielectrophoretic tweezers-based force spectroscopy inside a microfluidic device
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6210972/
https://www.ncbi.nlm.nih.gov/pubmed/30347732
http://dx.doi.org/10.3390/s18103543
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