Development and characterization of microsatellite markers in Gaultheria pumila Lf. (Ericaceae)

BACKGROUND: Polymorphic microsatellite markers were developed for Gaultheria pumila (Ericaceae) to evaluate genetic diversity and population structure within its native range in Chile. This is a very important Ericaceae endemic to Chile with a large commercial potential. Its resistance to different...

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Detalles Bibliográficos
Autores principales: Garcia-Gonzales, Rolando, Pico-Mendoza, José, Quiroz, Karla, Carrasco, Basilio, Cáceres, Pablo, Chong-Perez, Borys, Pino, Hugo, Seiltgens, Marjorie, Greck, Eglis, Caligari, Peter D. S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6211410/
https://www.ncbi.nlm.nih.gov/pubmed/30384861
http://dx.doi.org/10.1186/s40659-018-0193-4
Descripción
Sumario:BACKGROUND: Polymorphic microsatellite markers were developed for Gaultheria pumila (Ericaceae) to evaluate genetic diversity and population structure within its native range in Chile. This is a very important Ericaceae endemic to Chile with a large commercial potential. Its resistance to different abiotic conditions makes it a valuable target for genetic improvement. RESULTS: Ten polymorphic simple sequence repeat (SSR) loci were isolated from Gaultheria pumila using new-generation 454 FLX Titanium pyrosequencing technology. The mean number of alleles per locus ranged from 2 to 4. Observed and expected heterozygosity ranged from 0.00 to 1.0 and 0.00 to 0.64, respectively. CONCLUSIONS: From 10 SSR markers developed for G. pumila, 9 markers are promising candidates for analyzing genetic variation within or between natural populations of G. pumila and other species from the same genus.

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