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Single-particle imaging without symmetry constraints at an X-ray free-electron laser
The analysis of a single-particle imaging (SPI) experiment performed at the AMO beamline at LCLS as part of the SPI initiative is presented here. A workflow for the three-dimensional virus reconstruction of the PR772 bacteriophage from measured single-particle data is developed. It consists of sever...
Autores principales: | , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6211532/ https://www.ncbi.nlm.nih.gov/pubmed/30443357 http://dx.doi.org/10.1107/S205225251801120X |
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author | Rose, Max Bobkov, Sergey Ayyer, Kartik Kurta, Ruslan P. Dzhigaev, Dmitry Kim, Young Yong Morgan, Andrew J. Yoon, Chun Hong Westphal, Daniel Bielecki, Johan Sellberg, Jonas A. Williams, Garth Maia, Filipe R.N.C. Yefanov, Olexander M. Ilyin, Vyacheslav Mancuso, Adrian P. Chapman, Henry N. Hogue, Brenda G. Aquila, Andrew Barty, Anton Vartanyants, Ivan A. |
author_facet | Rose, Max Bobkov, Sergey Ayyer, Kartik Kurta, Ruslan P. Dzhigaev, Dmitry Kim, Young Yong Morgan, Andrew J. Yoon, Chun Hong Westphal, Daniel Bielecki, Johan Sellberg, Jonas A. Williams, Garth Maia, Filipe R.N.C. Yefanov, Olexander M. Ilyin, Vyacheslav Mancuso, Adrian P. Chapman, Henry N. Hogue, Brenda G. Aquila, Andrew Barty, Anton Vartanyants, Ivan A. |
author_sort | Rose, Max |
collection | PubMed |
description | The analysis of a single-particle imaging (SPI) experiment performed at the AMO beamline at LCLS as part of the SPI initiative is presented here. A workflow for the three-dimensional virus reconstruction of the PR772 bacteriophage from measured single-particle data is developed. It consists of several well defined steps including single-hit diffraction data classification, refined filtering of the classified data, reconstruction of three-dimensional scattered intensity from the experimental diffraction patterns by orientation determination and a final three-dimensional reconstruction of the virus electron density without symmetry constraints. The analysis developed here revealed and quantified nanoscale features of the PR772 virus measured in this experiment, with the obtained resolution better than 10 nm, with a clear indication that the structure was compressed in one direction and, as such, deviates from ideal icosahedral symmetry. |
format | Online Article Text |
id | pubmed-6211532 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-62115322018-11-15 Single-particle imaging without symmetry constraints at an X-ray free-electron laser Rose, Max Bobkov, Sergey Ayyer, Kartik Kurta, Ruslan P. Dzhigaev, Dmitry Kim, Young Yong Morgan, Andrew J. Yoon, Chun Hong Westphal, Daniel Bielecki, Johan Sellberg, Jonas A. Williams, Garth Maia, Filipe R.N.C. Yefanov, Olexander M. Ilyin, Vyacheslav Mancuso, Adrian P. Chapman, Henry N. Hogue, Brenda G. Aquila, Andrew Barty, Anton Vartanyants, Ivan A. IUCrJ Research Papers The analysis of a single-particle imaging (SPI) experiment performed at the AMO beamline at LCLS as part of the SPI initiative is presented here. A workflow for the three-dimensional virus reconstruction of the PR772 bacteriophage from measured single-particle data is developed. It consists of several well defined steps including single-hit diffraction data classification, refined filtering of the classified data, reconstruction of three-dimensional scattered intensity from the experimental diffraction patterns by orientation determination and a final three-dimensional reconstruction of the virus electron density without symmetry constraints. The analysis developed here revealed and quantified nanoscale features of the PR772 virus measured in this experiment, with the obtained resolution better than 10 nm, with a clear indication that the structure was compressed in one direction and, as such, deviates from ideal icosahedral symmetry. International Union of Crystallography 2018-09-18 /pmc/articles/PMC6211532/ /pubmed/30443357 http://dx.doi.org/10.1107/S205225251801120X Text en © Max Rose et al. 2018 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.http://creativecommons.org/licenses/by/2.0/uk/ |
spellingShingle | Research Papers Rose, Max Bobkov, Sergey Ayyer, Kartik Kurta, Ruslan P. Dzhigaev, Dmitry Kim, Young Yong Morgan, Andrew J. Yoon, Chun Hong Westphal, Daniel Bielecki, Johan Sellberg, Jonas A. Williams, Garth Maia, Filipe R.N.C. Yefanov, Olexander M. Ilyin, Vyacheslav Mancuso, Adrian P. Chapman, Henry N. Hogue, Brenda G. Aquila, Andrew Barty, Anton Vartanyants, Ivan A. Single-particle imaging without symmetry constraints at an X-ray free-electron laser |
title | Single-particle imaging without symmetry constraints at an X-ray free-electron laser |
title_full | Single-particle imaging without symmetry constraints at an X-ray free-electron laser |
title_fullStr | Single-particle imaging without symmetry constraints at an X-ray free-electron laser |
title_full_unstemmed | Single-particle imaging without symmetry constraints at an X-ray free-electron laser |
title_short | Single-particle imaging without symmetry constraints at an X-ray free-electron laser |
title_sort | single-particle imaging without symmetry constraints at an x-ray free-electron laser |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6211532/ https://www.ncbi.nlm.nih.gov/pubmed/30443357 http://dx.doi.org/10.1107/S205225251801120X |
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