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Single-particle imaging without symmetry constraints at an X-ray free-electron laser

The analysis of a single-particle imaging (SPI) experiment performed at the AMO beamline at LCLS as part of the SPI initiative is presented here. A workflow for the three-dimensional virus reconstruction of the PR772 bacteriophage from measured single-particle data is developed. It consists of sever...

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Autores principales: Rose, Max, Bobkov, Sergey, Ayyer, Kartik, Kurta, Ruslan P., Dzhigaev, Dmitry, Kim, Young Yong, Morgan, Andrew J., Yoon, Chun Hong, Westphal, Daniel, Bielecki, Johan, Sellberg, Jonas A., Williams, Garth, Maia, Filipe R.N.C., Yefanov, Olexander M., Ilyin, Vyacheslav, Mancuso, Adrian P., Chapman, Henry N., Hogue, Brenda G., Aquila, Andrew, Barty, Anton, Vartanyants, Ivan A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6211532/
https://www.ncbi.nlm.nih.gov/pubmed/30443357
http://dx.doi.org/10.1107/S205225251801120X
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author Rose, Max
Bobkov, Sergey
Ayyer, Kartik
Kurta, Ruslan P.
Dzhigaev, Dmitry
Kim, Young Yong
Morgan, Andrew J.
Yoon, Chun Hong
Westphal, Daniel
Bielecki, Johan
Sellberg, Jonas A.
Williams, Garth
Maia, Filipe R.N.C.
Yefanov, Olexander M.
Ilyin, Vyacheslav
Mancuso, Adrian P.
Chapman, Henry N.
Hogue, Brenda G.
Aquila, Andrew
Barty, Anton
Vartanyants, Ivan A.
author_facet Rose, Max
Bobkov, Sergey
Ayyer, Kartik
Kurta, Ruslan P.
Dzhigaev, Dmitry
Kim, Young Yong
Morgan, Andrew J.
Yoon, Chun Hong
Westphal, Daniel
Bielecki, Johan
Sellberg, Jonas A.
Williams, Garth
Maia, Filipe R.N.C.
Yefanov, Olexander M.
Ilyin, Vyacheslav
Mancuso, Adrian P.
Chapman, Henry N.
Hogue, Brenda G.
Aquila, Andrew
Barty, Anton
Vartanyants, Ivan A.
author_sort Rose, Max
collection PubMed
description The analysis of a single-particle imaging (SPI) experiment performed at the AMO beamline at LCLS as part of the SPI initiative is presented here. A workflow for the three-dimensional virus reconstruction of the PR772 bacteriophage from measured single-particle data is developed. It consists of several well defined steps including single-hit diffraction data classification, refined filtering of the classified data, reconstruction of three-dimensional scattered intensity from the experimental diffraction patterns by orientation determination and a final three-dimensional reconstruction of the virus electron density without symmetry constraints. The analysis developed here revealed and quantified nanoscale features of the PR772 virus measured in this experiment, with the obtained resolution better than 10 nm, with a clear indication that the structure was compressed in one direction and, as such, deviates from ideal icosahedral symmetry.
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spelling pubmed-62115322018-11-15 Single-particle imaging without symmetry constraints at an X-ray free-electron laser Rose, Max Bobkov, Sergey Ayyer, Kartik Kurta, Ruslan P. Dzhigaev, Dmitry Kim, Young Yong Morgan, Andrew J. Yoon, Chun Hong Westphal, Daniel Bielecki, Johan Sellberg, Jonas A. Williams, Garth Maia, Filipe R.N.C. Yefanov, Olexander M. Ilyin, Vyacheslav Mancuso, Adrian P. Chapman, Henry N. Hogue, Brenda G. Aquila, Andrew Barty, Anton Vartanyants, Ivan A. IUCrJ Research Papers The analysis of a single-particle imaging (SPI) experiment performed at the AMO beamline at LCLS as part of the SPI initiative is presented here. A workflow for the three-dimensional virus reconstruction of the PR772 bacteriophage from measured single-particle data is developed. It consists of several well defined steps including single-hit diffraction data classification, refined filtering of the classified data, reconstruction of three-dimensional scattered intensity from the experimental diffraction patterns by orientation determination and a final three-dimensional reconstruction of the virus electron density without symmetry constraints. The analysis developed here revealed and quantified nanoscale features of the PR772 virus measured in this experiment, with the obtained resolution better than 10 nm, with a clear indication that the structure was compressed in one direction and, as such, deviates from ideal icosahedral symmetry. International Union of Crystallography 2018-09-18 /pmc/articles/PMC6211532/ /pubmed/30443357 http://dx.doi.org/10.1107/S205225251801120X Text en © Max Rose et al. 2018 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.http://creativecommons.org/licenses/by/2.0/uk/
spellingShingle Research Papers
Rose, Max
Bobkov, Sergey
Ayyer, Kartik
Kurta, Ruslan P.
Dzhigaev, Dmitry
Kim, Young Yong
Morgan, Andrew J.
Yoon, Chun Hong
Westphal, Daniel
Bielecki, Johan
Sellberg, Jonas A.
Williams, Garth
Maia, Filipe R.N.C.
Yefanov, Olexander M.
Ilyin, Vyacheslav
Mancuso, Adrian P.
Chapman, Henry N.
Hogue, Brenda G.
Aquila, Andrew
Barty, Anton
Vartanyants, Ivan A.
Single-particle imaging without symmetry constraints at an X-ray free-electron laser
title Single-particle imaging without symmetry constraints at an X-ray free-electron laser
title_full Single-particle imaging without symmetry constraints at an X-ray free-electron laser
title_fullStr Single-particle imaging without symmetry constraints at an X-ray free-electron laser
title_full_unstemmed Single-particle imaging without symmetry constraints at an X-ray free-electron laser
title_short Single-particle imaging without symmetry constraints at an X-ray free-electron laser
title_sort single-particle imaging without symmetry constraints at an x-ray free-electron laser
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6211532/
https://www.ncbi.nlm.nih.gov/pubmed/30443357
http://dx.doi.org/10.1107/S205225251801120X
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