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Photocage-initiated time-resolved solution X-ray scattering investigation of protein dimerization
This work demonstrates a new method for investigating time-resolved structural changes in protein conformation and oligomerization via photocage-initiated time-resolved X-ray solution scattering by observing the ATP-driven dimerization of the MsbA nucleotide-binding domain. Photocaged small molecule...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6211537/ https://www.ncbi.nlm.nih.gov/pubmed/30443351 http://dx.doi.org/10.1107/S2052252518012149 |
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author | Josts, Inokentijs Niebling, Stephan Gao, Yunyun Levantino, Matteo Tidow, Henning Monteiro, Diana |
author_facet | Josts, Inokentijs Niebling, Stephan Gao, Yunyun Levantino, Matteo Tidow, Henning Monteiro, Diana |
author_sort | Josts, Inokentijs |
collection | PubMed |
description | This work demonstrates a new method for investigating time-resolved structural changes in protein conformation and oligomerization via photocage-initiated time-resolved X-ray solution scattering by observing the ATP-driven dimerization of the MsbA nucleotide-binding domain. Photocaged small molecules allow the observation of single-turnover reactions of non-naturally photoactivatable proteins. The kinetics of the reaction can be derived from changes in X-ray scattering associated with ATP-binding and subsequent dimerization. This method can be expanded to any small-molecule-driven protein reaction with conformational changes traceable by X-ray scattering where the small molecule can be photocaged. |
format | Online Article Text |
id | pubmed-6211537 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-62115372018-11-15 Photocage-initiated time-resolved solution X-ray scattering investigation of protein dimerization Josts, Inokentijs Niebling, Stephan Gao, Yunyun Levantino, Matteo Tidow, Henning Monteiro, Diana IUCrJ Research Letters This work demonstrates a new method for investigating time-resolved structural changes in protein conformation and oligomerization via photocage-initiated time-resolved X-ray solution scattering by observing the ATP-driven dimerization of the MsbA nucleotide-binding domain. Photocaged small molecules allow the observation of single-turnover reactions of non-naturally photoactivatable proteins. The kinetics of the reaction can be derived from changes in X-ray scattering associated with ATP-binding and subsequent dimerization. This method can be expanded to any small-molecule-driven protein reaction with conformational changes traceable by X-ray scattering where the small molecule can be photocaged. International Union of Crystallography 2018-09-13 /pmc/articles/PMC6211537/ /pubmed/30443351 http://dx.doi.org/10.1107/S2052252518012149 Text en © Inokentijs Josts et al. 2018 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.http://creativecommons.org/licenses/by/2.0/uk/ |
spellingShingle | Research Letters Josts, Inokentijs Niebling, Stephan Gao, Yunyun Levantino, Matteo Tidow, Henning Monteiro, Diana Photocage-initiated time-resolved solution X-ray scattering investigation of protein dimerization |
title | Photocage-initiated time-resolved solution X-ray scattering investigation of protein dimerization |
title_full | Photocage-initiated time-resolved solution X-ray scattering investigation of protein dimerization |
title_fullStr | Photocage-initiated time-resolved solution X-ray scattering investigation of protein dimerization |
title_full_unstemmed | Photocage-initiated time-resolved solution X-ray scattering investigation of protein dimerization |
title_short | Photocage-initiated time-resolved solution X-ray scattering investigation of protein dimerization |
title_sort | photocage-initiated time-resolved solution x-ray scattering investigation of protein dimerization |
topic | Research Letters |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6211537/ https://www.ncbi.nlm.nih.gov/pubmed/30443351 http://dx.doi.org/10.1107/S2052252518012149 |
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