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Effects of concurrent chloroquine and ethanol administration on the rat kidney morphology
INTRODUCTION: The use of antimalarial chloroquine in malaria-endemic regions of Africa is rampant and it is not uncommon to find individuals taken the drug concurrent with alcohol. Effects of anti-malarial drug chloroquine (Chq) and ethanol (Et) combination on kidney volume and function using rat mo...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The African Field Epidemiology Network
2018
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6211805/ https://www.ncbi.nlm.nih.gov/pubmed/30402202 http://dx.doi.org/10.11604/pamj.2018.29.49.12471 |
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author | Abdulkadir, Abdurrahman Mbajiorgu, Ejikeme Felix Nyirenda, Trust |
author_facet | Abdulkadir, Abdurrahman Mbajiorgu, Ejikeme Felix Nyirenda, Trust |
author_sort | Abdulkadir, Abdurrahman |
collection | PubMed |
description | INTRODUCTION: The use of antimalarial chloroquine in malaria-endemic regions of Africa is rampant and it is not uncommon to find individuals taken the drug concurrent with alcohol. Effects of anti-malarial drug chloroquine (Chq) and ethanol (Et) combination on kidney volume and function using rat model was investigated. METHODS: 32 adult male rats were randomly distributed into four groups of 8 rats each. Group C serve as control and received vehicle only, while Q is Chq treated only, E is Et treated and QE is Et and Chq treated. Chq was administered intraperitoneally at 1mg/100g body weight weekly and 6% Et in drinking water provided ad libitum. Urine volume was collected before the treatment began and after the treatment. After eight weeks, all animals were euthanized; kidneys were harvested and fixed in 10% neutral formalin. The fixed left kidneys were scanned with computed tomography and the scan slices were used to estimate 3-dimensional kidney volume on ImageJ. RESULTS: Total kidney volume was none significantly increased in Q, E and QE treated compared to control groups (p = 0.5150). Also, microscopic analysis showed increased proximal tubule diameter (p = 0.1426) and epithelial hypertrophy (p = 0.2530) and significant urinary space shrinkage (p = 0.00001). The initial urine volume was not significantly different between the control and treated groups (p = 0.9864) however, following treatment urine volume was significantly reduced in QE rats group (p = 0.0029). CONCLUSION: The results suggest chloroquine and ethanol combination as potential cause of kidney injury through structural damage and function derangement. |
format | Online Article Text |
id | pubmed-6211805 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | The African Field Epidemiology Network |
record_format | MEDLINE/PubMed |
spelling | pubmed-62118052018-11-06 Effects of concurrent chloroquine and ethanol administration on the rat kidney morphology Abdulkadir, Abdurrahman Mbajiorgu, Ejikeme Felix Nyirenda, Trust Pan Afr Med J Research INTRODUCTION: The use of antimalarial chloroquine in malaria-endemic regions of Africa is rampant and it is not uncommon to find individuals taken the drug concurrent with alcohol. Effects of anti-malarial drug chloroquine (Chq) and ethanol (Et) combination on kidney volume and function using rat model was investigated. METHODS: 32 adult male rats were randomly distributed into four groups of 8 rats each. Group C serve as control and received vehicle only, while Q is Chq treated only, E is Et treated and QE is Et and Chq treated. Chq was administered intraperitoneally at 1mg/100g body weight weekly and 6% Et in drinking water provided ad libitum. Urine volume was collected before the treatment began and after the treatment. After eight weeks, all animals were euthanized; kidneys were harvested and fixed in 10% neutral formalin. The fixed left kidneys were scanned with computed tomography and the scan slices were used to estimate 3-dimensional kidney volume on ImageJ. RESULTS: Total kidney volume was none significantly increased in Q, E and QE treated compared to control groups (p = 0.5150). Also, microscopic analysis showed increased proximal tubule diameter (p = 0.1426) and epithelial hypertrophy (p = 0.2530) and significant urinary space shrinkage (p = 0.00001). The initial urine volume was not significantly different between the control and treated groups (p = 0.9864) however, following treatment urine volume was significantly reduced in QE rats group (p = 0.0029). CONCLUSION: The results suggest chloroquine and ethanol combination as potential cause of kidney injury through structural damage and function derangement. The African Field Epidemiology Network 2018-01-18 /pmc/articles/PMC6211805/ /pubmed/30402202 http://dx.doi.org/10.11604/pamj.2018.29.49.12471 Text en © Abdurrahman Abdulkadir et al. http://creativecommons.org/licenses/by/2.0/ The Pan African Medical Journal - ISSN 1937-8688. This is an Open Access article distributed under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Abdulkadir, Abdurrahman Mbajiorgu, Ejikeme Felix Nyirenda, Trust Effects of concurrent chloroquine and ethanol administration on the rat kidney morphology |
title | Effects of concurrent chloroquine and ethanol administration on the rat kidney morphology |
title_full | Effects of concurrent chloroquine and ethanol administration on the rat kidney morphology |
title_fullStr | Effects of concurrent chloroquine and ethanol administration on the rat kidney morphology |
title_full_unstemmed | Effects of concurrent chloroquine and ethanol administration on the rat kidney morphology |
title_short | Effects of concurrent chloroquine and ethanol administration on the rat kidney morphology |
title_sort | effects of concurrent chloroquine and ethanol administration on the rat kidney morphology |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6211805/ https://www.ncbi.nlm.nih.gov/pubmed/30402202 http://dx.doi.org/10.11604/pamj.2018.29.49.12471 |
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