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Uranium (VI) detection in groundwater using a gold nanoparticle/paper-based lateral flow device
The contamination in groundwater due to the presence of uranium is nowadays a subject of concern due to the severe health problems associated with renal failure, genotoxicity and cancer. The standard methods to detect uranium require time-consuming processes and expensive non-portable equipment, so...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6212437/ https://www.ncbi.nlm.nih.gov/pubmed/30385866 http://dx.doi.org/10.1038/s41598-018-34610-5 |
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author | Quesada-González, Daniel Jairo, Grace A. Blake, Robert C. Blake, Diane A. Merkoçi, Arben |
author_facet | Quesada-González, Daniel Jairo, Grace A. Blake, Robert C. Blake, Diane A. Merkoçi, Arben |
author_sort | Quesada-González, Daniel |
collection | PubMed |
description | The contamination in groundwater due to the presence of uranium is nowadays a subject of concern due to the severe health problems associated with renal failure, genotoxicity and cancer. The standard methods to detect uranium require time-consuming processes and expensive non-portable equipment, so these measurements are rarely performed in-field, which increases the time until water samples are analysed. Furthermore, the few portable methods available do not allow quantitative analysis and the detection limit is often not low enough to reach the recommendations for drinking water (30 ppb or 126 nM of uranium). For the first time, we propose a portable, fast, inexpensive and sensitive paper-based biosensor able to detect in situ U(VI) in water samples: U(VI) selective gold nanoparticle-based lateral flow strips. Antibody-coated gold nanoparticles are used as labels in the proposed lateral flow system because of their biocompatibility; in addition, these nanoparticles provide high sensitivity due to their intense plasmonic effect. The antibody used in the assay recognizes soluble U(VI) complexed to the chelator, 2,9-dicarboxyl-1,10-phenanthroline (DCP). Because of the small size of the U(VI)-DCP complex, this assay employs a competitive format that reaches a limit of detection of 36.38 nM, lower than the action level (126 nM) established by the World Health Organization and the U.S. Environmental Protection Agency for drinking waters. |
format | Online Article Text |
id | pubmed-6212437 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-62124372018-11-06 Uranium (VI) detection in groundwater using a gold nanoparticle/paper-based lateral flow device Quesada-González, Daniel Jairo, Grace A. Blake, Robert C. Blake, Diane A. Merkoçi, Arben Sci Rep Article The contamination in groundwater due to the presence of uranium is nowadays a subject of concern due to the severe health problems associated with renal failure, genotoxicity and cancer. The standard methods to detect uranium require time-consuming processes and expensive non-portable equipment, so these measurements are rarely performed in-field, which increases the time until water samples are analysed. Furthermore, the few portable methods available do not allow quantitative analysis and the detection limit is often not low enough to reach the recommendations for drinking water (30 ppb or 126 nM of uranium). For the first time, we propose a portable, fast, inexpensive and sensitive paper-based biosensor able to detect in situ U(VI) in water samples: U(VI) selective gold nanoparticle-based lateral flow strips. Antibody-coated gold nanoparticles are used as labels in the proposed lateral flow system because of their biocompatibility; in addition, these nanoparticles provide high sensitivity due to their intense plasmonic effect. The antibody used in the assay recognizes soluble U(VI) complexed to the chelator, 2,9-dicarboxyl-1,10-phenanthroline (DCP). Because of the small size of the U(VI)-DCP complex, this assay employs a competitive format that reaches a limit of detection of 36.38 nM, lower than the action level (126 nM) established by the World Health Organization and the U.S. Environmental Protection Agency for drinking waters. Nature Publishing Group UK 2018-11-01 /pmc/articles/PMC6212437/ /pubmed/30385866 http://dx.doi.org/10.1038/s41598-018-34610-5 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Quesada-González, Daniel Jairo, Grace A. Blake, Robert C. Blake, Diane A. Merkoçi, Arben Uranium (VI) detection in groundwater using a gold nanoparticle/paper-based lateral flow device |
title | Uranium (VI) detection in groundwater using a gold nanoparticle/paper-based lateral flow device |
title_full | Uranium (VI) detection in groundwater using a gold nanoparticle/paper-based lateral flow device |
title_fullStr | Uranium (VI) detection in groundwater using a gold nanoparticle/paper-based lateral flow device |
title_full_unstemmed | Uranium (VI) detection in groundwater using a gold nanoparticle/paper-based lateral flow device |
title_short | Uranium (VI) detection in groundwater using a gold nanoparticle/paper-based lateral flow device |
title_sort | uranium (vi) detection in groundwater using a gold nanoparticle/paper-based lateral flow device |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6212437/ https://www.ncbi.nlm.nih.gov/pubmed/30385866 http://dx.doi.org/10.1038/s41598-018-34610-5 |
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