TRPM7 and MagT1 in the osteogenic differentiation of human mesenchymal stem cells in vitro

Mesenchymal stem cells are fundamental for bone formation and repair since they respond to microenvironmental stimuli by undergoing osteogenic differentiation. We show that the kinase and cation channel TRPM7 and the magnesium transporter MagT1 have a role in harmonizing the osteogenic differentiati...

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Autores principales: Castiglioni, Sara, Romeo, Valentina, Locatelli, Laura, Cazzaniga, Alessandra, Maier, Jeanette A. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6212439/
https://www.ncbi.nlm.nih.gov/pubmed/30385806
http://dx.doi.org/10.1038/s41598-018-34324-8
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author Castiglioni, Sara
Romeo, Valentina
Locatelli, Laura
Cazzaniga, Alessandra
Maier, Jeanette A. M.
author_facet Castiglioni, Sara
Romeo, Valentina
Locatelli, Laura
Cazzaniga, Alessandra
Maier, Jeanette A. M.
author_sort Castiglioni, Sara
collection PubMed
description Mesenchymal stem cells are fundamental for bone formation and repair since they respond to microenvironmental stimuli by undergoing osteogenic differentiation. We show that the kinase and cation channel TRPM7 and the magnesium transporter MagT1 have a role in harmonizing the osteogenic differentiation of human mesenchymal stem cells. TRPM7 and MagT1 are upregulated in osteogenic differentiation and silencing either one accelerates osteogenic differentiation, partly through the activation of autophagy. Intriguingly, similar results were obtained when the cells were cultured under magnesium deficient conditions. These results underpin the contribution of magnesium, TRPM7 and MagT1 to autophagy and osteoblastogenesis.
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spelling pubmed-62124392018-11-06 TRPM7 and MagT1 in the osteogenic differentiation of human mesenchymal stem cells in vitro Castiglioni, Sara Romeo, Valentina Locatelli, Laura Cazzaniga, Alessandra Maier, Jeanette A. M. Sci Rep Article Mesenchymal stem cells are fundamental for bone formation and repair since they respond to microenvironmental stimuli by undergoing osteogenic differentiation. We show that the kinase and cation channel TRPM7 and the magnesium transporter MagT1 have a role in harmonizing the osteogenic differentiation of human mesenchymal stem cells. TRPM7 and MagT1 are upregulated in osteogenic differentiation and silencing either one accelerates osteogenic differentiation, partly through the activation of autophagy. Intriguingly, similar results were obtained when the cells were cultured under magnesium deficient conditions. These results underpin the contribution of magnesium, TRPM7 and MagT1 to autophagy and osteoblastogenesis. Nature Publishing Group UK 2018-11-01 /pmc/articles/PMC6212439/ /pubmed/30385806 http://dx.doi.org/10.1038/s41598-018-34324-8 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Castiglioni, Sara
Romeo, Valentina
Locatelli, Laura
Cazzaniga, Alessandra
Maier, Jeanette A. M.
TRPM7 and MagT1 in the osteogenic differentiation of human mesenchymal stem cells in vitro
title TRPM7 and MagT1 in the osteogenic differentiation of human mesenchymal stem cells in vitro
title_full TRPM7 and MagT1 in the osteogenic differentiation of human mesenchymal stem cells in vitro
title_fullStr TRPM7 and MagT1 in the osteogenic differentiation of human mesenchymal stem cells in vitro
title_full_unstemmed TRPM7 and MagT1 in the osteogenic differentiation of human mesenchymal stem cells in vitro
title_short TRPM7 and MagT1 in the osteogenic differentiation of human mesenchymal stem cells in vitro
title_sort trpm7 and magt1 in the osteogenic differentiation of human mesenchymal stem cells in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6212439/
https://www.ncbi.nlm.nih.gov/pubmed/30385806
http://dx.doi.org/10.1038/s41598-018-34324-8
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