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Visualization of a Dinoflagellate-Infecting Virus HcDNAV and Its Infection Process

HcDNAV (a type species of Genus Dinodnavirus) is a large double-stranded DNA virus, which lytically infects the bloom-forming marine microalga Heterocapsa circularisquama Horiguchi (Dinophyceae). In the present study, detailed observation of the HcDNAV particle and its infection process was conducte...

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Autores principales: Takano, Yoshihito, Tomaru, Yuji, Nagasaki, Keizo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6212932/
https://www.ncbi.nlm.nih.gov/pubmed/30314306
http://dx.doi.org/10.3390/v10100554
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author Takano, Yoshihito
Tomaru, Yuji
Nagasaki, Keizo
author_facet Takano, Yoshihito
Tomaru, Yuji
Nagasaki, Keizo
author_sort Takano, Yoshihito
collection PubMed
description HcDNAV (a type species of Genus Dinodnavirus) is a large double-stranded DNA virus, which lytically infects the bloom-forming marine microalga Heterocapsa circularisquama Horiguchi (Dinophyceae). In the present study, detailed observation of the HcDNAV particle and its infection process was conducted via field emission scanning electron microscopy (FE-SEM) and epifluorescence microscopy (EFM). Each five-fold vertex of the icosahedral virion was decorated with a protrusion, which may be related to the entry process of HcDNAV into the host. The transverse groove of host cells is proposed to be the main virus entry site. A visible DAPI-stained region, which is considered to be the viroplasm (virus factory), appeared in close proximity to the host nucleus at 11 h post infection (hpi); the putative viral DAPI signal was remarkably enlarged at 11–30 hpi. It was kidney-shaped at 13–15 hpi, horseshoe-shaped at 20 hpi, doughnut-shaped at 30 hpi, and changed into a three-dimensionally complicated shape at 51–53 hpi, by which time most parts of the host cell were occupied by the putative viral DAPI signal. While the virions were within the viroplasm, they were easily distinguishable by their vertex protrusions by FE-SEM.
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spelling pubmed-62129322018-11-09 Visualization of a Dinoflagellate-Infecting Virus HcDNAV and Its Infection Process Takano, Yoshihito Tomaru, Yuji Nagasaki, Keizo Viruses Communication HcDNAV (a type species of Genus Dinodnavirus) is a large double-stranded DNA virus, which lytically infects the bloom-forming marine microalga Heterocapsa circularisquama Horiguchi (Dinophyceae). In the present study, detailed observation of the HcDNAV particle and its infection process was conducted via field emission scanning electron microscopy (FE-SEM) and epifluorescence microscopy (EFM). Each five-fold vertex of the icosahedral virion was decorated with a protrusion, which may be related to the entry process of HcDNAV into the host. The transverse groove of host cells is proposed to be the main virus entry site. A visible DAPI-stained region, which is considered to be the viroplasm (virus factory), appeared in close proximity to the host nucleus at 11 h post infection (hpi); the putative viral DAPI signal was remarkably enlarged at 11–30 hpi. It was kidney-shaped at 13–15 hpi, horseshoe-shaped at 20 hpi, doughnut-shaped at 30 hpi, and changed into a three-dimensionally complicated shape at 51–53 hpi, by which time most parts of the host cell were occupied by the putative viral DAPI signal. While the virions were within the viroplasm, they were easily distinguishable by their vertex protrusions by FE-SEM. MDPI 2018-10-11 /pmc/articles/PMC6212932/ /pubmed/30314306 http://dx.doi.org/10.3390/v10100554 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Takano, Yoshihito
Tomaru, Yuji
Nagasaki, Keizo
Visualization of a Dinoflagellate-Infecting Virus HcDNAV and Its Infection Process
title Visualization of a Dinoflagellate-Infecting Virus HcDNAV and Its Infection Process
title_full Visualization of a Dinoflagellate-Infecting Virus HcDNAV and Its Infection Process
title_fullStr Visualization of a Dinoflagellate-Infecting Virus HcDNAV and Its Infection Process
title_full_unstemmed Visualization of a Dinoflagellate-Infecting Virus HcDNAV and Its Infection Process
title_short Visualization of a Dinoflagellate-Infecting Virus HcDNAV and Its Infection Process
title_sort visualization of a dinoflagellate-infecting virus hcdnav and its infection process
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6212932/
https://www.ncbi.nlm.nih.gov/pubmed/30314306
http://dx.doi.org/10.3390/v10100554
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