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Induction of Trained Innate Immunity in Human Monocytes by Bovine Milk and Milk-Derived Immunoglobulin G

Innate immune memory, also termed “trained immunity” in vertebrates, has been recently described in a large variety of plants and animals. In most cases, trained innate immunity is induced by pathogens or pathogen-associated molecular patterns (PAMPs), and is associated with long-term epigenetic, me...

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Autores principales: van Splunter, Marloes, van Osch, Thijs L. J., Brugman, Sylvia, Savelkoul, Huub F. J., Joosten, Leo A. B., Netea, Mihai G., van Neerven, R. J. Joost
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6213000/
https://www.ncbi.nlm.nih.gov/pubmed/30262772
http://dx.doi.org/10.3390/nu10101378
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author van Splunter, Marloes
van Osch, Thijs L. J.
Brugman, Sylvia
Savelkoul, Huub F. J.
Joosten, Leo A. B.
Netea, Mihai G.
van Neerven, R. J. Joost
author_facet van Splunter, Marloes
van Osch, Thijs L. J.
Brugman, Sylvia
Savelkoul, Huub F. J.
Joosten, Leo A. B.
Netea, Mihai G.
van Neerven, R. J. Joost
author_sort van Splunter, Marloes
collection PubMed
description Innate immune memory, also termed “trained immunity” in vertebrates, has been recently described in a large variety of plants and animals. In most cases, trained innate immunity is induced by pathogens or pathogen-associated molecular patterns (PAMPs), and is associated with long-term epigenetic, metabolic, and functional reprogramming. Interestingly, recent findings indicate that food components can mimic PAMPs effects and induce trained immunity. The aim of this study was to investigate whether bovine milk or its components can induce trained immunity in human monocytes. To this aim, monocytes were exposed for 24 h to β-glucan, Toll-like receptor (TLR)-ligands, bovine milk, milk fractions, bovine lactoferrin (bLF), and bovine Immunoglobulin G (bIgG). After washing away the stimulus and a resting period of five days, the cells were re-stimulated with TLR ligands and Tumor necrosis factor (TNF-) and interleukin (IL)-6 production was measured. Training with β-glucan resulted in higher cytokine production after TLR1/2, TLR4, and TLR7/8 stimulation. When monocytes trained with raw milk were re-stimulated with TLR1/2 ligand Pam3CSK4, trained cells produced more IL-6 compared to non-trained cells. Training with bIgG resulted in higher cytokine production after TLR4 and TLR7/8 stimulation. These results show that bovine milk and bIgG can induce trained immunity in human monocytes. This confirms the hypothesis that diet components can influence the long-term responsiveness of the innate immune system.
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spelling pubmed-62130002018-11-06 Induction of Trained Innate Immunity in Human Monocytes by Bovine Milk and Milk-Derived Immunoglobulin G van Splunter, Marloes van Osch, Thijs L. J. Brugman, Sylvia Savelkoul, Huub F. J. Joosten, Leo A. B. Netea, Mihai G. van Neerven, R. J. Joost Nutrients Article Innate immune memory, also termed “trained immunity” in vertebrates, has been recently described in a large variety of plants and animals. In most cases, trained innate immunity is induced by pathogens or pathogen-associated molecular patterns (PAMPs), and is associated with long-term epigenetic, metabolic, and functional reprogramming. Interestingly, recent findings indicate that food components can mimic PAMPs effects and induce trained immunity. The aim of this study was to investigate whether bovine milk or its components can induce trained immunity in human monocytes. To this aim, monocytes were exposed for 24 h to β-glucan, Toll-like receptor (TLR)-ligands, bovine milk, milk fractions, bovine lactoferrin (bLF), and bovine Immunoglobulin G (bIgG). After washing away the stimulus and a resting period of five days, the cells were re-stimulated with TLR ligands and Tumor necrosis factor (TNF-) and interleukin (IL)-6 production was measured. Training with β-glucan resulted in higher cytokine production after TLR1/2, TLR4, and TLR7/8 stimulation. When monocytes trained with raw milk were re-stimulated with TLR1/2 ligand Pam3CSK4, trained cells produced more IL-6 compared to non-trained cells. Training with bIgG resulted in higher cytokine production after TLR4 and TLR7/8 stimulation. These results show that bovine milk and bIgG can induce trained immunity in human monocytes. This confirms the hypothesis that diet components can influence the long-term responsiveness of the innate immune system. MDPI 2018-09-27 /pmc/articles/PMC6213000/ /pubmed/30262772 http://dx.doi.org/10.3390/nu10101378 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
van Splunter, Marloes
van Osch, Thijs L. J.
Brugman, Sylvia
Savelkoul, Huub F. J.
Joosten, Leo A. B.
Netea, Mihai G.
van Neerven, R. J. Joost
Induction of Trained Innate Immunity in Human Monocytes by Bovine Milk and Milk-Derived Immunoglobulin G
title Induction of Trained Innate Immunity in Human Monocytes by Bovine Milk and Milk-Derived Immunoglobulin G
title_full Induction of Trained Innate Immunity in Human Monocytes by Bovine Milk and Milk-Derived Immunoglobulin G
title_fullStr Induction of Trained Innate Immunity in Human Monocytes by Bovine Milk and Milk-Derived Immunoglobulin G
title_full_unstemmed Induction of Trained Innate Immunity in Human Monocytes by Bovine Milk and Milk-Derived Immunoglobulin G
title_short Induction of Trained Innate Immunity in Human Monocytes by Bovine Milk and Milk-Derived Immunoglobulin G
title_sort induction of trained innate immunity in human monocytes by bovine milk and milk-derived immunoglobulin g
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6213000/
https://www.ncbi.nlm.nih.gov/pubmed/30262772
http://dx.doi.org/10.3390/nu10101378
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