Cargando…
Analysis of Gut Microbiota in Rheumatoid Arthritis Patients: Disease-Related Dysbiosis and Modifications Induced by Etanercept
A certain number of studies were carried out to address the question of how dysbiosis could affect the onset and development of rheumatoid arthritis (RA), but little is known about the reciprocal influence between microbiota composition and immunosuppressive drugs, and how this interaction may have...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6213034/ https://www.ncbi.nlm.nih.gov/pubmed/30261687 http://dx.doi.org/10.3390/ijms19102938 |
_version_ | 1783367678770020352 |
---|---|
author | Picchianti-Diamanti, Andrea Panebianco, Concetta Salemi, Simonetta Sorgi, Maria Laura Di Rosa, Roberta Tropea, Alessandro Sgrulletti, Mayla Salerno, Gerardo Terracciano, Fulvia D’Amelio, Raffaele Laganà, Bruno Pazienza, Valerio |
author_facet | Picchianti-Diamanti, Andrea Panebianco, Concetta Salemi, Simonetta Sorgi, Maria Laura Di Rosa, Roberta Tropea, Alessandro Sgrulletti, Mayla Salerno, Gerardo Terracciano, Fulvia D’Amelio, Raffaele Laganà, Bruno Pazienza, Valerio |
author_sort | Picchianti-Diamanti, Andrea |
collection | PubMed |
description | A certain number of studies were carried out to address the question of how dysbiosis could affect the onset and development of rheumatoid arthritis (RA), but little is known about the reciprocal influence between microbiota composition and immunosuppressive drugs, and how this interaction may have an impact on the clinical outcome. The aim of this study was to characterize the intestinal microbiota in a groups of RA patients treatment-naïve, under methotrexate, and/or etanercept (ETN). Correlations between the gut microbiota composition and validated immunological and clinical parameters of disease activity were also evaluated. In the current study, a 16S analysis was employed to explore the gut microbiota of 42 patients affected by RA and 10 healthy controls. Disease activity score on 28 joints (DAS-28), erythrocyte sedimentation rate, C-reactive protein, rheumatoid factor, anti-cyclic citrullinated peptides, and dietary and smoking habits were assessed. The composition of the gut microbiota in RA patients free of therapy is characterized by several abnormalities compared to healthy controls. Gut dysbiosis in RA patients is associated with different serological and clinical parameters; in particular, the phylum of Euryarchaeota was directly correlated to DAS and emerged as an independent risk factor. Patients under treatment with ETN present a partial restoration of a beneficial microbiota. The results of our study confirm that gut dysbiosis is a hallmark of the disease, and shows, for the first time, that the anti-tumor necrosis factor alpha (TNF-α) ETN is able to modify microbial communities, at least partially restoring a beneficial microbiota. |
format | Online Article Text |
id | pubmed-6213034 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62130342018-11-14 Analysis of Gut Microbiota in Rheumatoid Arthritis Patients: Disease-Related Dysbiosis and Modifications Induced by Etanercept Picchianti-Diamanti, Andrea Panebianco, Concetta Salemi, Simonetta Sorgi, Maria Laura Di Rosa, Roberta Tropea, Alessandro Sgrulletti, Mayla Salerno, Gerardo Terracciano, Fulvia D’Amelio, Raffaele Laganà, Bruno Pazienza, Valerio Int J Mol Sci Article A certain number of studies were carried out to address the question of how dysbiosis could affect the onset and development of rheumatoid arthritis (RA), but little is known about the reciprocal influence between microbiota composition and immunosuppressive drugs, and how this interaction may have an impact on the clinical outcome. The aim of this study was to characterize the intestinal microbiota in a groups of RA patients treatment-naïve, under methotrexate, and/or etanercept (ETN). Correlations between the gut microbiota composition and validated immunological and clinical parameters of disease activity were also evaluated. In the current study, a 16S analysis was employed to explore the gut microbiota of 42 patients affected by RA and 10 healthy controls. Disease activity score on 28 joints (DAS-28), erythrocyte sedimentation rate, C-reactive protein, rheumatoid factor, anti-cyclic citrullinated peptides, and dietary and smoking habits were assessed. The composition of the gut microbiota in RA patients free of therapy is characterized by several abnormalities compared to healthy controls. Gut dysbiosis in RA patients is associated with different serological and clinical parameters; in particular, the phylum of Euryarchaeota was directly correlated to DAS and emerged as an independent risk factor. Patients under treatment with ETN present a partial restoration of a beneficial microbiota. The results of our study confirm that gut dysbiosis is a hallmark of the disease, and shows, for the first time, that the anti-tumor necrosis factor alpha (TNF-α) ETN is able to modify microbial communities, at least partially restoring a beneficial microbiota. MDPI 2018-09-27 /pmc/articles/PMC6213034/ /pubmed/30261687 http://dx.doi.org/10.3390/ijms19102938 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Picchianti-Diamanti, Andrea Panebianco, Concetta Salemi, Simonetta Sorgi, Maria Laura Di Rosa, Roberta Tropea, Alessandro Sgrulletti, Mayla Salerno, Gerardo Terracciano, Fulvia D’Amelio, Raffaele Laganà, Bruno Pazienza, Valerio Analysis of Gut Microbiota in Rheumatoid Arthritis Patients: Disease-Related Dysbiosis and Modifications Induced by Etanercept |
title | Analysis of Gut Microbiota in Rheumatoid Arthritis Patients: Disease-Related Dysbiosis and Modifications Induced by Etanercept |
title_full | Analysis of Gut Microbiota in Rheumatoid Arthritis Patients: Disease-Related Dysbiosis and Modifications Induced by Etanercept |
title_fullStr | Analysis of Gut Microbiota in Rheumatoid Arthritis Patients: Disease-Related Dysbiosis and Modifications Induced by Etanercept |
title_full_unstemmed | Analysis of Gut Microbiota in Rheumatoid Arthritis Patients: Disease-Related Dysbiosis and Modifications Induced by Etanercept |
title_short | Analysis of Gut Microbiota in Rheumatoid Arthritis Patients: Disease-Related Dysbiosis and Modifications Induced by Etanercept |
title_sort | analysis of gut microbiota in rheumatoid arthritis patients: disease-related dysbiosis and modifications induced by etanercept |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6213034/ https://www.ncbi.nlm.nih.gov/pubmed/30261687 http://dx.doi.org/10.3390/ijms19102938 |
work_keys_str_mv | AT picchiantidiamantiandrea analysisofgutmicrobiotainrheumatoidarthritispatientsdiseaserelateddysbiosisandmodificationsinducedbyetanercept AT panebiancoconcetta analysisofgutmicrobiotainrheumatoidarthritispatientsdiseaserelateddysbiosisandmodificationsinducedbyetanercept AT salemisimonetta analysisofgutmicrobiotainrheumatoidarthritispatientsdiseaserelateddysbiosisandmodificationsinducedbyetanercept AT sorgimarialaura analysisofgutmicrobiotainrheumatoidarthritispatientsdiseaserelateddysbiosisandmodificationsinducedbyetanercept AT dirosaroberta analysisofgutmicrobiotainrheumatoidarthritispatientsdiseaserelateddysbiosisandmodificationsinducedbyetanercept AT tropeaalessandro analysisofgutmicrobiotainrheumatoidarthritispatientsdiseaserelateddysbiosisandmodificationsinducedbyetanercept AT sgrullettimayla analysisofgutmicrobiotainrheumatoidarthritispatientsdiseaserelateddysbiosisandmodificationsinducedbyetanercept AT salernogerardo analysisofgutmicrobiotainrheumatoidarthritispatientsdiseaserelateddysbiosisandmodificationsinducedbyetanercept AT terraccianofulvia analysisofgutmicrobiotainrheumatoidarthritispatientsdiseaserelateddysbiosisandmodificationsinducedbyetanercept AT damelioraffaele analysisofgutmicrobiotainrheumatoidarthritispatientsdiseaserelateddysbiosisandmodificationsinducedbyetanercept AT laganabruno analysisofgutmicrobiotainrheumatoidarthritispatientsdiseaserelateddysbiosisandmodificationsinducedbyetanercept AT pazienzavalerio analysisofgutmicrobiotainrheumatoidarthritispatientsdiseaserelateddysbiosisandmodificationsinducedbyetanercept |