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Optimization of Growth and Carotenoid Production by Haloferax mediterranei Using Response Surface Methodology

Haloferax mediterranei produces C50 carotenoids that have strong antioxidant properties. The response surface methodology (RSM) tool helps to accurately analyze the most suitable conditions to maximize C50 carotenoids production by haloarchaea. The effects of temperature (15–50 °C), pH (4−10), and s...

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Autores principales: Montero-Lobato, Zaida, Ramos-Merchante, Adrián, Fuentes, Juan Luis, Sayago, Ana, Fernández-Recamales, Ángeles, Martínez-Espinosa, Rosa María, Vega, José María, Vílchez, Carlos, Garbayo, Inés
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6213265/
https://www.ncbi.nlm.nih.gov/pubmed/30304770
http://dx.doi.org/10.3390/md16100372
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author Montero-Lobato, Zaida
Ramos-Merchante, Adrián
Fuentes, Juan Luis
Sayago, Ana
Fernández-Recamales, Ángeles
Martínez-Espinosa, Rosa María
Vega, José María
Vílchez, Carlos
Garbayo, Inés
author_facet Montero-Lobato, Zaida
Ramos-Merchante, Adrián
Fuentes, Juan Luis
Sayago, Ana
Fernández-Recamales, Ángeles
Martínez-Espinosa, Rosa María
Vega, José María
Vílchez, Carlos
Garbayo, Inés
author_sort Montero-Lobato, Zaida
collection PubMed
description Haloferax mediterranei produces C50 carotenoids that have strong antioxidant properties. The response surface methodology (RSM) tool helps to accurately analyze the most suitable conditions to maximize C50 carotenoids production by haloarchaea. The effects of temperature (15–50 °C), pH (4−10), and salinity (5–28% NaCl (w/v)) on the growth and carotenoid content of H. mediterranei were analyzed using the RSM approach. Growth was determined by measuring the turbidity at 600 nm. To determine the carotenoid content, harvested cells were lysed by freeze/thawing, then re-suspended in acetone and the total carotenoid content determined by measuring the absorbance at 494 nm. The analysis of carotenoids was performed by an HPLC system coupled with mass spectrometry. The results indicated the theoretical optimal conditions of 36.51 or 36.81 °C, pH of 8.20 or 8.96, and 15.01% or 12.03% (w/v) salinity for the growth of haloarchaea (OD600 = 12.5 ± 0.64) and production of total carotenoids (3.34 ± 0.29 mg/L), respectively. These conditions were validated experimentally for growth (OD600 = 13.72 ± 0.98) and carotenoid production (3.74 ± 0.20 mg/L). The carotenoid profile showed four isomers of bacterioruberin (89.13%). Our findings suggest that the RSM approach is highly useful for determining optimal conditions for large-scale production of bacterioruberin by haloarchaea.
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spelling pubmed-62132652018-11-09 Optimization of Growth and Carotenoid Production by Haloferax mediterranei Using Response Surface Methodology Montero-Lobato, Zaida Ramos-Merchante, Adrián Fuentes, Juan Luis Sayago, Ana Fernández-Recamales, Ángeles Martínez-Espinosa, Rosa María Vega, José María Vílchez, Carlos Garbayo, Inés Mar Drugs Article Haloferax mediterranei produces C50 carotenoids that have strong antioxidant properties. The response surface methodology (RSM) tool helps to accurately analyze the most suitable conditions to maximize C50 carotenoids production by haloarchaea. The effects of temperature (15–50 °C), pH (4−10), and salinity (5–28% NaCl (w/v)) on the growth and carotenoid content of H. mediterranei were analyzed using the RSM approach. Growth was determined by measuring the turbidity at 600 nm. To determine the carotenoid content, harvested cells were lysed by freeze/thawing, then re-suspended in acetone and the total carotenoid content determined by measuring the absorbance at 494 nm. The analysis of carotenoids was performed by an HPLC system coupled with mass spectrometry. The results indicated the theoretical optimal conditions of 36.51 or 36.81 °C, pH of 8.20 or 8.96, and 15.01% or 12.03% (w/v) salinity for the growth of haloarchaea (OD600 = 12.5 ± 0.64) and production of total carotenoids (3.34 ± 0.29 mg/L), respectively. These conditions were validated experimentally for growth (OD600 = 13.72 ± 0.98) and carotenoid production (3.74 ± 0.20 mg/L). The carotenoid profile showed four isomers of bacterioruberin (89.13%). Our findings suggest that the RSM approach is highly useful for determining optimal conditions for large-scale production of bacterioruberin by haloarchaea. MDPI 2018-10-09 /pmc/articles/PMC6213265/ /pubmed/30304770 http://dx.doi.org/10.3390/md16100372 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Montero-Lobato, Zaida
Ramos-Merchante, Adrián
Fuentes, Juan Luis
Sayago, Ana
Fernández-Recamales, Ángeles
Martínez-Espinosa, Rosa María
Vega, José María
Vílchez, Carlos
Garbayo, Inés
Optimization of Growth and Carotenoid Production by Haloferax mediterranei Using Response Surface Methodology
title Optimization of Growth and Carotenoid Production by Haloferax mediterranei Using Response Surface Methodology
title_full Optimization of Growth and Carotenoid Production by Haloferax mediterranei Using Response Surface Methodology
title_fullStr Optimization of Growth and Carotenoid Production by Haloferax mediterranei Using Response Surface Methodology
title_full_unstemmed Optimization of Growth and Carotenoid Production by Haloferax mediterranei Using Response Surface Methodology
title_short Optimization of Growth and Carotenoid Production by Haloferax mediterranei Using Response Surface Methodology
title_sort optimization of growth and carotenoid production by haloferax mediterranei using response surface methodology
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6213265/
https://www.ncbi.nlm.nih.gov/pubmed/30304770
http://dx.doi.org/10.3390/md16100372
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