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Optimization of Growth and Carotenoid Production by Haloferax mediterranei Using Response Surface Methodology
Haloferax mediterranei produces C50 carotenoids that have strong antioxidant properties. The response surface methodology (RSM) tool helps to accurately analyze the most suitable conditions to maximize C50 carotenoids production by haloarchaea. The effects of temperature (15–50 °C), pH (4−10), and s...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6213265/ https://www.ncbi.nlm.nih.gov/pubmed/30304770 http://dx.doi.org/10.3390/md16100372 |
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author | Montero-Lobato, Zaida Ramos-Merchante, Adrián Fuentes, Juan Luis Sayago, Ana Fernández-Recamales, Ángeles Martínez-Espinosa, Rosa María Vega, José María Vílchez, Carlos Garbayo, Inés |
author_facet | Montero-Lobato, Zaida Ramos-Merchante, Adrián Fuentes, Juan Luis Sayago, Ana Fernández-Recamales, Ángeles Martínez-Espinosa, Rosa María Vega, José María Vílchez, Carlos Garbayo, Inés |
author_sort | Montero-Lobato, Zaida |
collection | PubMed |
description | Haloferax mediterranei produces C50 carotenoids that have strong antioxidant properties. The response surface methodology (RSM) tool helps to accurately analyze the most suitable conditions to maximize C50 carotenoids production by haloarchaea. The effects of temperature (15–50 °C), pH (4−10), and salinity (5–28% NaCl (w/v)) on the growth and carotenoid content of H. mediterranei were analyzed using the RSM approach. Growth was determined by measuring the turbidity at 600 nm. To determine the carotenoid content, harvested cells were lysed by freeze/thawing, then re-suspended in acetone and the total carotenoid content determined by measuring the absorbance at 494 nm. The analysis of carotenoids was performed by an HPLC system coupled with mass spectrometry. The results indicated the theoretical optimal conditions of 36.51 or 36.81 °C, pH of 8.20 or 8.96, and 15.01% or 12.03% (w/v) salinity for the growth of haloarchaea (OD600 = 12.5 ± 0.64) and production of total carotenoids (3.34 ± 0.29 mg/L), respectively. These conditions were validated experimentally for growth (OD600 = 13.72 ± 0.98) and carotenoid production (3.74 ± 0.20 mg/L). The carotenoid profile showed four isomers of bacterioruberin (89.13%). Our findings suggest that the RSM approach is highly useful for determining optimal conditions for large-scale production of bacterioruberin by haloarchaea. |
format | Online Article Text |
id | pubmed-6213265 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62132652018-11-09 Optimization of Growth and Carotenoid Production by Haloferax mediterranei Using Response Surface Methodology Montero-Lobato, Zaida Ramos-Merchante, Adrián Fuentes, Juan Luis Sayago, Ana Fernández-Recamales, Ángeles Martínez-Espinosa, Rosa María Vega, José María Vílchez, Carlos Garbayo, Inés Mar Drugs Article Haloferax mediterranei produces C50 carotenoids that have strong antioxidant properties. The response surface methodology (RSM) tool helps to accurately analyze the most suitable conditions to maximize C50 carotenoids production by haloarchaea. The effects of temperature (15–50 °C), pH (4−10), and salinity (5–28% NaCl (w/v)) on the growth and carotenoid content of H. mediterranei were analyzed using the RSM approach. Growth was determined by measuring the turbidity at 600 nm. To determine the carotenoid content, harvested cells were lysed by freeze/thawing, then re-suspended in acetone and the total carotenoid content determined by measuring the absorbance at 494 nm. The analysis of carotenoids was performed by an HPLC system coupled with mass spectrometry. The results indicated the theoretical optimal conditions of 36.51 or 36.81 °C, pH of 8.20 or 8.96, and 15.01% or 12.03% (w/v) salinity for the growth of haloarchaea (OD600 = 12.5 ± 0.64) and production of total carotenoids (3.34 ± 0.29 mg/L), respectively. These conditions were validated experimentally for growth (OD600 = 13.72 ± 0.98) and carotenoid production (3.74 ± 0.20 mg/L). The carotenoid profile showed four isomers of bacterioruberin (89.13%). Our findings suggest that the RSM approach is highly useful for determining optimal conditions for large-scale production of bacterioruberin by haloarchaea. MDPI 2018-10-09 /pmc/articles/PMC6213265/ /pubmed/30304770 http://dx.doi.org/10.3390/md16100372 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Montero-Lobato, Zaida Ramos-Merchante, Adrián Fuentes, Juan Luis Sayago, Ana Fernández-Recamales, Ángeles Martínez-Espinosa, Rosa María Vega, José María Vílchez, Carlos Garbayo, Inés Optimization of Growth and Carotenoid Production by Haloferax mediterranei Using Response Surface Methodology |
title | Optimization of Growth and Carotenoid Production by Haloferax mediterranei Using Response Surface Methodology |
title_full | Optimization of Growth and Carotenoid Production by Haloferax mediterranei Using Response Surface Methodology |
title_fullStr | Optimization of Growth and Carotenoid Production by Haloferax mediterranei Using Response Surface Methodology |
title_full_unstemmed | Optimization of Growth and Carotenoid Production by Haloferax mediterranei Using Response Surface Methodology |
title_short | Optimization of Growth and Carotenoid Production by Haloferax mediterranei Using Response Surface Methodology |
title_sort | optimization of growth and carotenoid production by haloferax mediterranei using response surface methodology |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6213265/ https://www.ncbi.nlm.nih.gov/pubmed/30304770 http://dx.doi.org/10.3390/md16100372 |
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