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Sandensolide Induces Oxidative Stress-Mediated Apoptosis in Oral Cancer Cells and in Zebrafish Xenograft Model

Presently, natural sources and herbs are being sought for the treatment of human oral squamous cell carcinoma (OSCC) in order to alleviate the side effects of chemotherapy. This study investigates the effect of sandensolide, a cembrane isolated from Sinularia flexibilis, to inhibit human OSCC cell g...

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Autores principales: Yu, Chung-I, Chen, Chung-Yi, Liu, Wangta, Chang, Po-Chih, Huang, Chiung-Wei, Han, Kuang-Fen, Lin, In-Pin, Lin, Mei-Ying, Lee, Chien-Hsing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6213332/
https://www.ncbi.nlm.nih.gov/pubmed/30332851
http://dx.doi.org/10.3390/md16100387
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author Yu, Chung-I
Chen, Chung-Yi
Liu, Wangta
Chang, Po-Chih
Huang, Chiung-Wei
Han, Kuang-Fen
Lin, In-Pin
Lin, Mei-Ying
Lee, Chien-Hsing
author_facet Yu, Chung-I
Chen, Chung-Yi
Liu, Wangta
Chang, Po-Chih
Huang, Chiung-Wei
Han, Kuang-Fen
Lin, In-Pin
Lin, Mei-Ying
Lee, Chien-Hsing
author_sort Yu, Chung-I
collection PubMed
description Presently, natural sources and herbs are being sought for the treatment of human oral squamous cell carcinoma (OSCC) in order to alleviate the side effects of chemotherapy. This study investigates the effect of sandensolide, a cembrane isolated from Sinularia flexibilis, to inhibit human OSCC cell growth with the aim of developing a new drug for the treatment of oral cancer. In vitro cultured human OSCC models (Ca9.22, SCC9 and HSC-3 cell lines) and oral normal cells (HGF-1), as well as a zebrafish xenograft model, were used to test the cytotoxicity of sandensolide (MTT assay), as well as to perform cell cycle analysis and Western blotting. Both the in vitro bioassay and the zebrafish xenograft model demonstrated the anti-oral cancer effect of sandensolide. Moreover, sandensolide was able to significantly suppress colony formation and induce apoptosis, as well as cell cycle arrest, in OSCC by regulating multiple key proteins. Induction of reactive oxygen species (ROS) was observed in sandensolide-treated oral cancer cells. However, these apoptotic changes were rescued by NAC pretreatment. These findings contribute to the knowledge of the model of action of sandensolide, which may induce oxidative stress-mediated cell death pathways as a potential agent in oral cancer therapeutics.
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spelling pubmed-62133322018-11-09 Sandensolide Induces Oxidative Stress-Mediated Apoptosis in Oral Cancer Cells and in Zebrafish Xenograft Model Yu, Chung-I Chen, Chung-Yi Liu, Wangta Chang, Po-Chih Huang, Chiung-Wei Han, Kuang-Fen Lin, In-Pin Lin, Mei-Ying Lee, Chien-Hsing Mar Drugs Article Presently, natural sources and herbs are being sought for the treatment of human oral squamous cell carcinoma (OSCC) in order to alleviate the side effects of chemotherapy. This study investigates the effect of sandensolide, a cembrane isolated from Sinularia flexibilis, to inhibit human OSCC cell growth with the aim of developing a new drug for the treatment of oral cancer. In vitro cultured human OSCC models (Ca9.22, SCC9 and HSC-3 cell lines) and oral normal cells (HGF-1), as well as a zebrafish xenograft model, were used to test the cytotoxicity of sandensolide (MTT assay), as well as to perform cell cycle analysis and Western blotting. Both the in vitro bioassay and the zebrafish xenograft model demonstrated the anti-oral cancer effect of sandensolide. Moreover, sandensolide was able to significantly suppress colony formation and induce apoptosis, as well as cell cycle arrest, in OSCC by regulating multiple key proteins. Induction of reactive oxygen species (ROS) was observed in sandensolide-treated oral cancer cells. However, these apoptotic changes were rescued by NAC pretreatment. These findings contribute to the knowledge of the model of action of sandensolide, which may induce oxidative stress-mediated cell death pathways as a potential agent in oral cancer therapeutics. MDPI 2018-10-16 /pmc/articles/PMC6213332/ /pubmed/30332851 http://dx.doi.org/10.3390/md16100387 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yu, Chung-I
Chen, Chung-Yi
Liu, Wangta
Chang, Po-Chih
Huang, Chiung-Wei
Han, Kuang-Fen
Lin, In-Pin
Lin, Mei-Ying
Lee, Chien-Hsing
Sandensolide Induces Oxidative Stress-Mediated Apoptosis in Oral Cancer Cells and in Zebrafish Xenograft Model
title Sandensolide Induces Oxidative Stress-Mediated Apoptosis in Oral Cancer Cells and in Zebrafish Xenograft Model
title_full Sandensolide Induces Oxidative Stress-Mediated Apoptosis in Oral Cancer Cells and in Zebrafish Xenograft Model
title_fullStr Sandensolide Induces Oxidative Stress-Mediated Apoptosis in Oral Cancer Cells and in Zebrafish Xenograft Model
title_full_unstemmed Sandensolide Induces Oxidative Stress-Mediated Apoptosis in Oral Cancer Cells and in Zebrafish Xenograft Model
title_short Sandensolide Induces Oxidative Stress-Mediated Apoptosis in Oral Cancer Cells and in Zebrafish Xenograft Model
title_sort sandensolide induces oxidative stress-mediated apoptosis in oral cancer cells and in zebrafish xenograft model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6213332/
https://www.ncbi.nlm.nih.gov/pubmed/30332851
http://dx.doi.org/10.3390/md16100387
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