Cargando…
The Cocktail Effect of BMP-2 and TGF-β1 Loaded in Visible Light-Cured Glycol Chitosan Hydrogels for the Enhancement of Bone Formation in a Rat Tibial Defect Model
Bone tissue engineering scaffolds offer the merits of minimal invasion as well as localized and controlled biomolecule release to targeted sites. In this study, we prepared injectable hydrogel systems based on visible light-cured glycol chitosan (GC) hydrogels containing bone morphogenetic protein-2...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6213427/ https://www.ncbi.nlm.nih.gov/pubmed/30257482 http://dx.doi.org/10.3390/md16100351 |
_version_ | 1783367765744156672 |
---|---|
author | Yoon, Sun-Jung Yoo, Youngbum Nam, Sang Eun Hyun, Hoon Lee, Deok-Won Um, Sewook Kim, So Yeon Hong, Sung Ok Yang, Dae Hyeok Chun, Heung Jae |
author_facet | Yoon, Sun-Jung Yoo, Youngbum Nam, Sang Eun Hyun, Hoon Lee, Deok-Won Um, Sewook Kim, So Yeon Hong, Sung Ok Yang, Dae Hyeok Chun, Heung Jae |
author_sort | Yoon, Sun-Jung |
collection | PubMed |
description | Bone tissue engineering scaffolds offer the merits of minimal invasion as well as localized and controlled biomolecule release to targeted sites. In this study, we prepared injectable hydrogel systems based on visible light-cured glycol chitosan (GC) hydrogels containing bone morphogenetic protein-2 (BMP-2) and/or transforming growth factor-beta1 (TGF-β1) as scaffolds for bone formation in vitro and in vivo. The hydrogels were characterized by storage modulus, scanning electron microscopy (SEM) and swelling ratio analyses. The developed hydrogel systems showed controlled releases of growth factors in a sustained manner for 30 days. In vitro and in vivo studies revealed that growth factor-loaded GC hydrogels have no cytotoxicity against MC3T3-E1 osteoblast cell line, improved mRNA expressions of alkaline phosphatase (ALP), type I collagen (COL 1) and osteocalcin (OCN), and increased bone volume (BV) and bone mineral density (BMD) in tibia defect sites. Moreover, GC hydrogel containing BMP-2 (10 ng) and TGF-β1 (10 ng) (GC/BMP-2/TGF-β1-10 ng) showed greater bone formation abilities than that containing BMP-2 (5 ng) and TGF-β1 (5 ng) (GC/BMP-2/TGF-β1-5 ng) in vitro and in vivo. Consequently, the injectable GC/BMP-2/TGF-β1-10 ng hydrogel may have clinical potential for dental or orthopedic applications. |
format | Online Article Text |
id | pubmed-6213427 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62134272018-11-09 The Cocktail Effect of BMP-2 and TGF-β1 Loaded in Visible Light-Cured Glycol Chitosan Hydrogels for the Enhancement of Bone Formation in a Rat Tibial Defect Model Yoon, Sun-Jung Yoo, Youngbum Nam, Sang Eun Hyun, Hoon Lee, Deok-Won Um, Sewook Kim, So Yeon Hong, Sung Ok Yang, Dae Hyeok Chun, Heung Jae Mar Drugs Article Bone tissue engineering scaffolds offer the merits of minimal invasion as well as localized and controlled biomolecule release to targeted sites. In this study, we prepared injectable hydrogel systems based on visible light-cured glycol chitosan (GC) hydrogels containing bone morphogenetic protein-2 (BMP-2) and/or transforming growth factor-beta1 (TGF-β1) as scaffolds for bone formation in vitro and in vivo. The hydrogels were characterized by storage modulus, scanning electron microscopy (SEM) and swelling ratio analyses. The developed hydrogel systems showed controlled releases of growth factors in a sustained manner for 30 days. In vitro and in vivo studies revealed that growth factor-loaded GC hydrogels have no cytotoxicity against MC3T3-E1 osteoblast cell line, improved mRNA expressions of alkaline phosphatase (ALP), type I collagen (COL 1) and osteocalcin (OCN), and increased bone volume (BV) and bone mineral density (BMD) in tibia defect sites. Moreover, GC hydrogel containing BMP-2 (10 ng) and TGF-β1 (10 ng) (GC/BMP-2/TGF-β1-10 ng) showed greater bone formation abilities than that containing BMP-2 (5 ng) and TGF-β1 (5 ng) (GC/BMP-2/TGF-β1-5 ng) in vitro and in vivo. Consequently, the injectable GC/BMP-2/TGF-β1-10 ng hydrogel may have clinical potential for dental or orthopedic applications. MDPI 2018-09-25 /pmc/articles/PMC6213427/ /pubmed/30257482 http://dx.doi.org/10.3390/md16100351 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yoon, Sun-Jung Yoo, Youngbum Nam, Sang Eun Hyun, Hoon Lee, Deok-Won Um, Sewook Kim, So Yeon Hong, Sung Ok Yang, Dae Hyeok Chun, Heung Jae The Cocktail Effect of BMP-2 and TGF-β1 Loaded in Visible Light-Cured Glycol Chitosan Hydrogels for the Enhancement of Bone Formation in a Rat Tibial Defect Model |
title | The Cocktail Effect of BMP-2 and TGF-β1 Loaded in Visible Light-Cured Glycol Chitosan Hydrogels for the Enhancement of Bone Formation in a Rat Tibial Defect Model |
title_full | The Cocktail Effect of BMP-2 and TGF-β1 Loaded in Visible Light-Cured Glycol Chitosan Hydrogels for the Enhancement of Bone Formation in a Rat Tibial Defect Model |
title_fullStr | The Cocktail Effect of BMP-2 and TGF-β1 Loaded in Visible Light-Cured Glycol Chitosan Hydrogels for the Enhancement of Bone Formation in a Rat Tibial Defect Model |
title_full_unstemmed | The Cocktail Effect of BMP-2 and TGF-β1 Loaded in Visible Light-Cured Glycol Chitosan Hydrogels for the Enhancement of Bone Formation in a Rat Tibial Defect Model |
title_short | The Cocktail Effect of BMP-2 and TGF-β1 Loaded in Visible Light-Cured Glycol Chitosan Hydrogels for the Enhancement of Bone Formation in a Rat Tibial Defect Model |
title_sort | cocktail effect of bmp-2 and tgf-β1 loaded in visible light-cured glycol chitosan hydrogels for the enhancement of bone formation in a rat tibial defect model |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6213427/ https://www.ncbi.nlm.nih.gov/pubmed/30257482 http://dx.doi.org/10.3390/md16100351 |
work_keys_str_mv | AT yoonsunjung thecocktaileffectofbmp2andtgfb1loadedinvisiblelightcuredglycolchitosanhydrogelsfortheenhancementofboneformationinarattibialdefectmodel AT yooyoungbum thecocktaileffectofbmp2andtgfb1loadedinvisiblelightcuredglycolchitosanhydrogelsfortheenhancementofboneformationinarattibialdefectmodel AT namsangeun thecocktaileffectofbmp2andtgfb1loadedinvisiblelightcuredglycolchitosanhydrogelsfortheenhancementofboneformationinarattibialdefectmodel AT hyunhoon thecocktaileffectofbmp2andtgfb1loadedinvisiblelightcuredglycolchitosanhydrogelsfortheenhancementofboneformationinarattibialdefectmodel AT leedeokwon thecocktaileffectofbmp2andtgfb1loadedinvisiblelightcuredglycolchitosanhydrogelsfortheenhancementofboneformationinarattibialdefectmodel AT umsewook thecocktaileffectofbmp2andtgfb1loadedinvisiblelightcuredglycolchitosanhydrogelsfortheenhancementofboneformationinarattibialdefectmodel AT kimsoyeon thecocktaileffectofbmp2andtgfb1loadedinvisiblelightcuredglycolchitosanhydrogelsfortheenhancementofboneformationinarattibialdefectmodel AT hongsungok thecocktaileffectofbmp2andtgfb1loadedinvisiblelightcuredglycolchitosanhydrogelsfortheenhancementofboneformationinarattibialdefectmodel AT yangdaehyeok thecocktaileffectofbmp2andtgfb1loadedinvisiblelightcuredglycolchitosanhydrogelsfortheenhancementofboneformationinarattibialdefectmodel AT chunheungjae thecocktaileffectofbmp2andtgfb1loadedinvisiblelightcuredglycolchitosanhydrogelsfortheenhancementofboneformationinarattibialdefectmodel AT yoonsunjung cocktaileffectofbmp2andtgfb1loadedinvisiblelightcuredglycolchitosanhydrogelsfortheenhancementofboneformationinarattibialdefectmodel AT yooyoungbum cocktaileffectofbmp2andtgfb1loadedinvisiblelightcuredglycolchitosanhydrogelsfortheenhancementofboneformationinarattibialdefectmodel AT namsangeun cocktaileffectofbmp2andtgfb1loadedinvisiblelightcuredglycolchitosanhydrogelsfortheenhancementofboneformationinarattibialdefectmodel AT hyunhoon cocktaileffectofbmp2andtgfb1loadedinvisiblelightcuredglycolchitosanhydrogelsfortheenhancementofboneformationinarattibialdefectmodel AT leedeokwon cocktaileffectofbmp2andtgfb1loadedinvisiblelightcuredglycolchitosanhydrogelsfortheenhancementofboneformationinarattibialdefectmodel AT umsewook cocktaileffectofbmp2andtgfb1loadedinvisiblelightcuredglycolchitosanhydrogelsfortheenhancementofboneformationinarattibialdefectmodel AT kimsoyeon cocktaileffectofbmp2andtgfb1loadedinvisiblelightcuredglycolchitosanhydrogelsfortheenhancementofboneformationinarattibialdefectmodel AT hongsungok cocktaileffectofbmp2andtgfb1loadedinvisiblelightcuredglycolchitosanhydrogelsfortheenhancementofboneformationinarattibialdefectmodel AT yangdaehyeok cocktaileffectofbmp2andtgfb1loadedinvisiblelightcuredglycolchitosanhydrogelsfortheenhancementofboneformationinarattibialdefectmodel AT chunheungjae cocktaileffectofbmp2andtgfb1loadedinvisiblelightcuredglycolchitosanhydrogelsfortheenhancementofboneformationinarattibialdefectmodel |