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Correlation between Diabetes Mellitus and Knee Osteoarthritis: A Dry-To-Wet Lab Approach

Recent years have witnessed an increased prevalence of knee osteoarthritis (KOA) among diabetes mellitus (DM) patients—conditions which might share common risk factors such as obesity and advanced aging. Therefore, we conducted dry-to-wet lab research approaches to assess the correlation of type 1 D...

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Autores principales: Dubey, Navneet Kumar, Ningrum, Dina Nur Anggraini, Dubey, Rajni, Deng, Yue-Hua, Li, Yu-Chuan, Wang, Peter D., Wang, Joseph R., Syed-Abdul, Shabbir, Deng, Win-Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6213511/
https://www.ncbi.nlm.nih.gov/pubmed/30282957
http://dx.doi.org/10.3390/ijms19103021
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author Dubey, Navneet Kumar
Ningrum, Dina Nur Anggraini
Dubey, Rajni
Deng, Yue-Hua
Li, Yu-Chuan
Wang, Peter D.
Wang, Joseph R.
Syed-Abdul, Shabbir
Deng, Win-Ping
author_facet Dubey, Navneet Kumar
Ningrum, Dina Nur Anggraini
Dubey, Rajni
Deng, Yue-Hua
Li, Yu-Chuan
Wang, Peter D.
Wang, Joseph R.
Syed-Abdul, Shabbir
Deng, Win-Ping
author_sort Dubey, Navneet Kumar
collection PubMed
description Recent years have witnessed an increased prevalence of knee osteoarthritis (KOA) among diabetes mellitus (DM) patients—conditions which might share common risk factors such as obesity and advanced aging. Therefore, we conducted dry-to-wet lab research approaches to assess the correlation of type 1 DM (T1DM) and type 2 DM (T2DM) with KOA among all age and genders of Taiwanese population. The strength of association (odds ratio: OR) was analyzed using a phenome-wide association study portal. Populations of 37,353 T1DM and 1,218,254 T2DM were included. We observed a significant association of KOA with T1DM (OR: 1.40 (1.33–1.47), p< 0.0001) and T2DM (OR: 2.75 (2.72–2.78), p< 0.0001). The association between T1DM and KOA among the obese (OR: 0.99 (0.54–1.67), p = 0.0477) was insignificant compared to the non-obese (OR: 1.40 (1.33–1.48), p < 0.0001). Interestingly, a higher association between T2DM and KOA among non-obese persons (OR: 2.75, (2.72–2.79), p < 0.0001) compared to the obese (OR: 1.71 (1.55–1.89), p < 0.0001) was noted. Further, histopathologic and Western blot studies of diabetic mice knee joints revealed enhanced carboxymethyl lysine (advanced glycation end product), matrix metalloproteinase-1, and reduced cartilage-specific proteins, including type II collagen (Col II), SOX9, and aggrecan (AGN), indicating deteriorated articular cartilage and proteoglycans. Results indicate that DM is strongly associated with KOA, and obesity may not be a confounding factor.
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spelling pubmed-62135112018-11-14 Correlation between Diabetes Mellitus and Knee Osteoarthritis: A Dry-To-Wet Lab Approach Dubey, Navneet Kumar Ningrum, Dina Nur Anggraini Dubey, Rajni Deng, Yue-Hua Li, Yu-Chuan Wang, Peter D. Wang, Joseph R. Syed-Abdul, Shabbir Deng, Win-Ping Int J Mol Sci Article Recent years have witnessed an increased prevalence of knee osteoarthritis (KOA) among diabetes mellitus (DM) patients—conditions which might share common risk factors such as obesity and advanced aging. Therefore, we conducted dry-to-wet lab research approaches to assess the correlation of type 1 DM (T1DM) and type 2 DM (T2DM) with KOA among all age and genders of Taiwanese population. The strength of association (odds ratio: OR) was analyzed using a phenome-wide association study portal. Populations of 37,353 T1DM and 1,218,254 T2DM were included. We observed a significant association of KOA with T1DM (OR: 1.40 (1.33–1.47), p< 0.0001) and T2DM (OR: 2.75 (2.72–2.78), p< 0.0001). The association between T1DM and KOA among the obese (OR: 0.99 (0.54–1.67), p = 0.0477) was insignificant compared to the non-obese (OR: 1.40 (1.33–1.48), p < 0.0001). Interestingly, a higher association between T2DM and KOA among non-obese persons (OR: 2.75, (2.72–2.79), p < 0.0001) compared to the obese (OR: 1.71 (1.55–1.89), p < 0.0001) was noted. Further, histopathologic and Western blot studies of diabetic mice knee joints revealed enhanced carboxymethyl lysine (advanced glycation end product), matrix metalloproteinase-1, and reduced cartilage-specific proteins, including type II collagen (Col II), SOX9, and aggrecan (AGN), indicating deteriorated articular cartilage and proteoglycans. Results indicate that DM is strongly associated with KOA, and obesity may not be a confounding factor. MDPI 2018-10-03 /pmc/articles/PMC6213511/ /pubmed/30282957 http://dx.doi.org/10.3390/ijms19103021 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dubey, Navneet Kumar
Ningrum, Dina Nur Anggraini
Dubey, Rajni
Deng, Yue-Hua
Li, Yu-Chuan
Wang, Peter D.
Wang, Joseph R.
Syed-Abdul, Shabbir
Deng, Win-Ping
Correlation between Diabetes Mellitus and Knee Osteoarthritis: A Dry-To-Wet Lab Approach
title Correlation between Diabetes Mellitus and Knee Osteoarthritis: A Dry-To-Wet Lab Approach
title_full Correlation between Diabetes Mellitus and Knee Osteoarthritis: A Dry-To-Wet Lab Approach
title_fullStr Correlation between Diabetes Mellitus and Knee Osteoarthritis: A Dry-To-Wet Lab Approach
title_full_unstemmed Correlation between Diabetes Mellitus and Knee Osteoarthritis: A Dry-To-Wet Lab Approach
title_short Correlation between Diabetes Mellitus and Knee Osteoarthritis: A Dry-To-Wet Lab Approach
title_sort correlation between diabetes mellitus and knee osteoarthritis: a dry-to-wet lab approach
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6213511/
https://www.ncbi.nlm.nih.gov/pubmed/30282957
http://dx.doi.org/10.3390/ijms19103021
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