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Massive Gene Expansion and Sequence Diversification Is Associated with Diverse Tissue Distribution, Regulation and Antimicrobial Properties of Anti-Lipopolysaccharide Factors in Shrimp

Anti-lipopolysaccharide factors (ALFs) are antimicrobial peptides with a central β-hairpin structure able to bind to microbial components. Mining sequence databases for ALFs allowed us to show the remarkable diversity of ALF sequences in shrimp. We found at least seven members of the ALF family (Gro...

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Autores principales: Matos, Gabriel Machado, Schmitt, Paulina, Barreto, Cairé, Farias, Natanael Dantas, Toledo-Silva, Guilherme, Guzmán, Fanny, Destoumieux-Garzón, Delphine, Perazzolo, Luciane Maria, Rosa, Rafael Diego
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6213531/
https://www.ncbi.nlm.nih.gov/pubmed/30314303
http://dx.doi.org/10.3390/md16100381
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author Matos, Gabriel Machado
Schmitt, Paulina
Barreto, Cairé
Farias, Natanael Dantas
Toledo-Silva, Guilherme
Guzmán, Fanny
Destoumieux-Garzón, Delphine
Perazzolo, Luciane Maria
Rosa, Rafael Diego
author_facet Matos, Gabriel Machado
Schmitt, Paulina
Barreto, Cairé
Farias, Natanael Dantas
Toledo-Silva, Guilherme
Guzmán, Fanny
Destoumieux-Garzón, Delphine
Perazzolo, Luciane Maria
Rosa, Rafael Diego
author_sort Matos, Gabriel Machado
collection PubMed
description Anti-lipopolysaccharide factors (ALFs) are antimicrobial peptides with a central β-hairpin structure able to bind to microbial components. Mining sequence databases for ALFs allowed us to show the remarkable diversity of ALF sequences in shrimp. We found at least seven members of the ALF family (Groups A to G), including two novel Groups (F and G), all of which are encoded by different loci with conserved gene organization. Phylogenetic analyses revealed that gene expansion and subsequent diversification of the ALF family occurred in crustaceans before shrimp speciation occurred. The transcriptional profile of ALFs was compared in terms of tissue distribution, response to two pathogens and during shrimp development in Litopenaeus vannamei, the most cultivated species. ALFs were found to be constitutively expressed in hemocytes and to respond differently to tissue damage. While synthetic β-hairpins of Groups E and G displayed both antibacterial and antifungal activities, no activity was recorded for Group F β-hairpins. Altogether, our results showed that ALFs form a family of shrimp AMPs that has been the subject of intense diversification. The different genes differ in terms of tissue expression, regulation and function. These data strongly suggest that multiple selection pressures have led to functional diversification of ALFs in shrimp.
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spelling pubmed-62135312018-11-09 Massive Gene Expansion and Sequence Diversification Is Associated with Diverse Tissue Distribution, Regulation and Antimicrobial Properties of Anti-Lipopolysaccharide Factors in Shrimp Matos, Gabriel Machado Schmitt, Paulina Barreto, Cairé Farias, Natanael Dantas Toledo-Silva, Guilherme Guzmán, Fanny Destoumieux-Garzón, Delphine Perazzolo, Luciane Maria Rosa, Rafael Diego Mar Drugs Article Anti-lipopolysaccharide factors (ALFs) are antimicrobial peptides with a central β-hairpin structure able to bind to microbial components. Mining sequence databases for ALFs allowed us to show the remarkable diversity of ALF sequences in shrimp. We found at least seven members of the ALF family (Groups A to G), including two novel Groups (F and G), all of which are encoded by different loci with conserved gene organization. Phylogenetic analyses revealed that gene expansion and subsequent diversification of the ALF family occurred in crustaceans before shrimp speciation occurred. The transcriptional profile of ALFs was compared in terms of tissue distribution, response to two pathogens and during shrimp development in Litopenaeus vannamei, the most cultivated species. ALFs were found to be constitutively expressed in hemocytes and to respond differently to tissue damage. While synthetic β-hairpins of Groups E and G displayed both antibacterial and antifungal activities, no activity was recorded for Group F β-hairpins. Altogether, our results showed that ALFs form a family of shrimp AMPs that has been the subject of intense diversification. The different genes differ in terms of tissue expression, regulation and function. These data strongly suggest that multiple selection pressures have led to functional diversification of ALFs in shrimp. MDPI 2018-10-11 /pmc/articles/PMC6213531/ /pubmed/30314303 http://dx.doi.org/10.3390/md16100381 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Matos, Gabriel Machado
Schmitt, Paulina
Barreto, Cairé
Farias, Natanael Dantas
Toledo-Silva, Guilherme
Guzmán, Fanny
Destoumieux-Garzón, Delphine
Perazzolo, Luciane Maria
Rosa, Rafael Diego
Massive Gene Expansion and Sequence Diversification Is Associated with Diverse Tissue Distribution, Regulation and Antimicrobial Properties of Anti-Lipopolysaccharide Factors in Shrimp
title Massive Gene Expansion and Sequence Diversification Is Associated with Diverse Tissue Distribution, Regulation and Antimicrobial Properties of Anti-Lipopolysaccharide Factors in Shrimp
title_full Massive Gene Expansion and Sequence Diversification Is Associated with Diverse Tissue Distribution, Regulation and Antimicrobial Properties of Anti-Lipopolysaccharide Factors in Shrimp
title_fullStr Massive Gene Expansion and Sequence Diversification Is Associated with Diverse Tissue Distribution, Regulation and Antimicrobial Properties of Anti-Lipopolysaccharide Factors in Shrimp
title_full_unstemmed Massive Gene Expansion and Sequence Diversification Is Associated with Diverse Tissue Distribution, Regulation and Antimicrobial Properties of Anti-Lipopolysaccharide Factors in Shrimp
title_short Massive Gene Expansion and Sequence Diversification Is Associated with Diverse Tissue Distribution, Regulation and Antimicrobial Properties of Anti-Lipopolysaccharide Factors in Shrimp
title_sort massive gene expansion and sequence diversification is associated with diverse tissue distribution, regulation and antimicrobial properties of anti-lipopolysaccharide factors in shrimp
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6213531/
https://www.ncbi.nlm.nih.gov/pubmed/30314303
http://dx.doi.org/10.3390/md16100381
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