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Fetal Brain Infection Is Not a Unique Characteristic of Brazilian Zika Viruses

The recent emergence of Zika virus (ZIKV) in Brazil was associated with an increased number of fetal brain infections that resulted in a spectrum of congenital neurological complications known as congenital Zika syndrome (CZS). Herein, we generated de novo from sequence data an early Asian lineage Z...

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Autores principales: Setoh, Yin Xiang, Peng, Nias Y., Nakayama, Eri, Amarilla, Alberto A., Prow, Natalie A., Suhrbier, Andreas, Khromykh, Alexander A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6213914/
https://www.ncbi.nlm.nih.gov/pubmed/30282919
http://dx.doi.org/10.3390/v10100541
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author Setoh, Yin Xiang
Peng, Nias Y.
Nakayama, Eri
Amarilla, Alberto A.
Prow, Natalie A.
Suhrbier, Andreas
Khromykh, Alexander A.
author_facet Setoh, Yin Xiang
Peng, Nias Y.
Nakayama, Eri
Amarilla, Alberto A.
Prow, Natalie A.
Suhrbier, Andreas
Khromykh, Alexander A.
author_sort Setoh, Yin Xiang
collection PubMed
description The recent emergence of Zika virus (ZIKV) in Brazil was associated with an increased number of fetal brain infections that resulted in a spectrum of congenital neurological complications known as congenital Zika syndrome (CZS). Herein, we generated de novo from sequence data an early Asian lineage ZIKV isolate (ZIKV-MY; Malaysia, 1966) not associated with microcephaly and compared the in vitro replication kinetics and fetal brain infection in interferon α/β receptor 1 knockout (IFNAR1(−/−)) dams of this isolate and of a Brazilian isolate (ZIKV-Natal; Natal, 2015) unequivocally associated with microcephaly. The replication efficiencies of ZIKV-MY and ZIKV-Natal in A549 and Vero cells were similar, while ZIKV-MY replicated more efficiently in wild-type (WT) and IFNAR(−/−) mouse embryonic fibroblasts. Viremias in IFNAR1(−/−) dams were similar after infection with ZIKV-MY or ZIKV-Natal, and importantly, infection of fetal brains was also not significantly different. Thus, fetal brain infection does not appear to be a unique feature of Brazilian ZIKV isolates.
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spelling pubmed-62139142018-11-09 Fetal Brain Infection Is Not a Unique Characteristic of Brazilian Zika Viruses Setoh, Yin Xiang Peng, Nias Y. Nakayama, Eri Amarilla, Alberto A. Prow, Natalie A. Suhrbier, Andreas Khromykh, Alexander A. Viruses Communication The recent emergence of Zika virus (ZIKV) in Brazil was associated with an increased number of fetal brain infections that resulted in a spectrum of congenital neurological complications known as congenital Zika syndrome (CZS). Herein, we generated de novo from sequence data an early Asian lineage ZIKV isolate (ZIKV-MY; Malaysia, 1966) not associated with microcephaly and compared the in vitro replication kinetics and fetal brain infection in interferon α/β receptor 1 knockout (IFNAR1(−/−)) dams of this isolate and of a Brazilian isolate (ZIKV-Natal; Natal, 2015) unequivocally associated with microcephaly. The replication efficiencies of ZIKV-MY and ZIKV-Natal in A549 and Vero cells were similar, while ZIKV-MY replicated more efficiently in wild-type (WT) and IFNAR(−/−) mouse embryonic fibroblasts. Viremias in IFNAR1(−/−) dams were similar after infection with ZIKV-MY or ZIKV-Natal, and importantly, infection of fetal brains was also not significantly different. Thus, fetal brain infection does not appear to be a unique feature of Brazilian ZIKV isolates. MDPI 2018-10-03 /pmc/articles/PMC6213914/ /pubmed/30282919 http://dx.doi.org/10.3390/v10100541 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Setoh, Yin Xiang
Peng, Nias Y.
Nakayama, Eri
Amarilla, Alberto A.
Prow, Natalie A.
Suhrbier, Andreas
Khromykh, Alexander A.
Fetal Brain Infection Is Not a Unique Characteristic of Brazilian Zika Viruses
title Fetal Brain Infection Is Not a Unique Characteristic of Brazilian Zika Viruses
title_full Fetal Brain Infection Is Not a Unique Characteristic of Brazilian Zika Viruses
title_fullStr Fetal Brain Infection Is Not a Unique Characteristic of Brazilian Zika Viruses
title_full_unstemmed Fetal Brain Infection Is Not a Unique Characteristic of Brazilian Zika Viruses
title_short Fetal Brain Infection Is Not a Unique Characteristic of Brazilian Zika Viruses
title_sort fetal brain infection is not a unique characteristic of brazilian zika viruses
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6213914/
https://www.ncbi.nlm.nih.gov/pubmed/30282919
http://dx.doi.org/10.3390/v10100541
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