Characterization of MK(8)(H(2)) from Rhodococcus sp. B7740 and Its Potential Antiglycation Capacity Measurements

Menaquinone (MK) has an important role in human metabolism as an essential vitamin (VK(2)), which is mainly produced through the fermentation of microorganisms. MK(8)(H(2)) was identified to be the main menaquinone from Rhodococcus sp. B7740, a bacterium isolated from the arctic ocean. In this work, MK(8)(H(2)) (purity: 99.75%) was collected through a convenient and economic extraction process followed by high-speed countercurrent chromatography (HSCCC) purification. Additionally, high-resolution mass spectrometry (HRMS) was performed for further identification and the hydrogenation position of MK(8)(H(2)) (terminal unit) was determined using nuclear magnetic resonance (NMR) for the first time. MK(8)(H(2)) showed a superior antioxidant effect and antiglycation capacity compared with ubiquinone Q10 and MK(4). High-performance liquid chromatography–mass spectrometer (HPLC-MS/MS) and molecular docking showed the fine interaction between MK(8)(H(2)) with methylglyoxal (MGO) and bull serum albumin (BSA), respectively. These properties make MK(8)(H(2)) a promising natural active ingredient with future food and medicine applications.