Cargando…

Identification of Arbuscular Mycorrhiza Fungi Responsive microRNAs and Their Regulatory Network in Maize

Maize can form symbiotic relationships with arbuscular mycorrhiza (AM) fungus to increase productivity and resistance, but the miRNAs in maize responsible for this process have not been discovered. In this study, 155 known and 28 novel miRNAs were identified by performing high-throughput sequencing...

Descripción completa

Detalles Bibliográficos
Autores principales: Xu, Yunjian, Zhu, Suwen, Liu, Fang, Wang, Wei, Wang, Xuewen, Han, Guomin, Cheng, Beijiu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6214007/
https://www.ncbi.nlm.nih.gov/pubmed/30332850
http://dx.doi.org/10.3390/ijms19103201
_version_ 1783367908502536192
author Xu, Yunjian
Zhu, Suwen
Liu, Fang
Wang, Wei
Wang, Xuewen
Han, Guomin
Cheng, Beijiu
author_facet Xu, Yunjian
Zhu, Suwen
Liu, Fang
Wang, Wei
Wang, Xuewen
Han, Guomin
Cheng, Beijiu
author_sort Xu, Yunjian
collection PubMed
description Maize can form symbiotic relationships with arbuscular mycorrhiza (AM) fungus to increase productivity and resistance, but the miRNAs in maize responsible for this process have not been discovered. In this study, 155 known and 28 novel miRNAs were identified by performing high-throughput sequencing of sRNA in maize roots colonized by AM fungi. Similar to the profiles in other AM-capable plants, a large proportion of identified maize miRNAs were 24 nt in length. Fourteen and two miRNAs were significantly down- and up-regulated in response to AM fungus Glomus intraradices inoculation, respectively, suggesting potential roles of these miRNAs in AM symbiosis. Interestingly, 12 of 14 significantly down-regulated known maize miRNAs belong to the miR399 family, which was previously reported to be involved in the interaction between Medicago truncatula and AM fungi. This result indicated that the miR399 family should regulate AM symbiosis conservatively across different plant lineages. Pathway and network analyses showed that the differentially expressed miRNAs might regulate lipid metabolism and phosphate starvation response in maize during the symbiosis process via their target genes. Several members of the miR399 family and the miR397 family should be involved in controlling the fatty acid metabolism and promoting lipid delivering from plants to AM fungi. To the best of our knowledge, this is the first report on miRNAs mediating fatty acids from plant to AM fungi. This study provides insight into the regulatory roles of miRNAs in the symbiosis between plants and AM fungi.
format Online
Article
Text
id pubmed-6214007
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-62140072018-11-14 Identification of Arbuscular Mycorrhiza Fungi Responsive microRNAs and Their Regulatory Network in Maize Xu, Yunjian Zhu, Suwen Liu, Fang Wang, Wei Wang, Xuewen Han, Guomin Cheng, Beijiu Int J Mol Sci Article Maize can form symbiotic relationships with arbuscular mycorrhiza (AM) fungus to increase productivity and resistance, but the miRNAs in maize responsible for this process have not been discovered. In this study, 155 known and 28 novel miRNAs were identified by performing high-throughput sequencing of sRNA in maize roots colonized by AM fungi. Similar to the profiles in other AM-capable plants, a large proportion of identified maize miRNAs were 24 nt in length. Fourteen and two miRNAs were significantly down- and up-regulated in response to AM fungus Glomus intraradices inoculation, respectively, suggesting potential roles of these miRNAs in AM symbiosis. Interestingly, 12 of 14 significantly down-regulated known maize miRNAs belong to the miR399 family, which was previously reported to be involved in the interaction between Medicago truncatula and AM fungi. This result indicated that the miR399 family should regulate AM symbiosis conservatively across different plant lineages. Pathway and network analyses showed that the differentially expressed miRNAs might regulate lipid metabolism and phosphate starvation response in maize during the symbiosis process via their target genes. Several members of the miR399 family and the miR397 family should be involved in controlling the fatty acid metabolism and promoting lipid delivering from plants to AM fungi. To the best of our knowledge, this is the first report on miRNAs mediating fatty acids from plant to AM fungi. This study provides insight into the regulatory roles of miRNAs in the symbiosis between plants and AM fungi. MDPI 2018-10-16 /pmc/articles/PMC6214007/ /pubmed/30332850 http://dx.doi.org/10.3390/ijms19103201 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Xu, Yunjian
Zhu, Suwen
Liu, Fang
Wang, Wei
Wang, Xuewen
Han, Guomin
Cheng, Beijiu
Identification of Arbuscular Mycorrhiza Fungi Responsive microRNAs and Their Regulatory Network in Maize
title Identification of Arbuscular Mycorrhiza Fungi Responsive microRNAs and Their Regulatory Network in Maize
title_full Identification of Arbuscular Mycorrhiza Fungi Responsive microRNAs and Their Regulatory Network in Maize
title_fullStr Identification of Arbuscular Mycorrhiza Fungi Responsive microRNAs and Their Regulatory Network in Maize
title_full_unstemmed Identification of Arbuscular Mycorrhiza Fungi Responsive microRNAs and Their Regulatory Network in Maize
title_short Identification of Arbuscular Mycorrhiza Fungi Responsive microRNAs and Their Regulatory Network in Maize
title_sort identification of arbuscular mycorrhiza fungi responsive micrornas and their regulatory network in maize
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6214007/
https://www.ncbi.nlm.nih.gov/pubmed/30332850
http://dx.doi.org/10.3390/ijms19103201
work_keys_str_mv AT xuyunjian identificationofarbuscularmycorrhizafungiresponsivemicrornasandtheirregulatorynetworkinmaize
AT zhusuwen identificationofarbuscularmycorrhizafungiresponsivemicrornasandtheirregulatorynetworkinmaize
AT liufang identificationofarbuscularmycorrhizafungiresponsivemicrornasandtheirregulatorynetworkinmaize
AT wangwei identificationofarbuscularmycorrhizafungiresponsivemicrornasandtheirregulatorynetworkinmaize
AT wangxuewen identificationofarbuscularmycorrhizafungiresponsivemicrornasandtheirregulatorynetworkinmaize
AT hanguomin identificationofarbuscularmycorrhizafungiresponsivemicrornasandtheirregulatorynetworkinmaize
AT chengbeijiu identificationofarbuscularmycorrhizafungiresponsivemicrornasandtheirregulatorynetworkinmaize