Cargando…
Identification of Arbuscular Mycorrhiza Fungi Responsive microRNAs and Their Regulatory Network in Maize
Maize can form symbiotic relationships with arbuscular mycorrhiza (AM) fungus to increase productivity and resistance, but the miRNAs in maize responsible for this process have not been discovered. In this study, 155 known and 28 novel miRNAs were identified by performing high-throughput sequencing...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6214007/ https://www.ncbi.nlm.nih.gov/pubmed/30332850 http://dx.doi.org/10.3390/ijms19103201 |
_version_ | 1783367908502536192 |
---|---|
author | Xu, Yunjian Zhu, Suwen Liu, Fang Wang, Wei Wang, Xuewen Han, Guomin Cheng, Beijiu |
author_facet | Xu, Yunjian Zhu, Suwen Liu, Fang Wang, Wei Wang, Xuewen Han, Guomin Cheng, Beijiu |
author_sort | Xu, Yunjian |
collection | PubMed |
description | Maize can form symbiotic relationships with arbuscular mycorrhiza (AM) fungus to increase productivity and resistance, but the miRNAs in maize responsible for this process have not been discovered. In this study, 155 known and 28 novel miRNAs were identified by performing high-throughput sequencing of sRNA in maize roots colonized by AM fungi. Similar to the profiles in other AM-capable plants, a large proportion of identified maize miRNAs were 24 nt in length. Fourteen and two miRNAs were significantly down- and up-regulated in response to AM fungus Glomus intraradices inoculation, respectively, suggesting potential roles of these miRNAs in AM symbiosis. Interestingly, 12 of 14 significantly down-regulated known maize miRNAs belong to the miR399 family, which was previously reported to be involved in the interaction between Medicago truncatula and AM fungi. This result indicated that the miR399 family should regulate AM symbiosis conservatively across different plant lineages. Pathway and network analyses showed that the differentially expressed miRNAs might regulate lipid metabolism and phosphate starvation response in maize during the symbiosis process via their target genes. Several members of the miR399 family and the miR397 family should be involved in controlling the fatty acid metabolism and promoting lipid delivering from plants to AM fungi. To the best of our knowledge, this is the first report on miRNAs mediating fatty acids from plant to AM fungi. This study provides insight into the regulatory roles of miRNAs in the symbiosis between plants and AM fungi. |
format | Online Article Text |
id | pubmed-6214007 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62140072018-11-14 Identification of Arbuscular Mycorrhiza Fungi Responsive microRNAs and Their Regulatory Network in Maize Xu, Yunjian Zhu, Suwen Liu, Fang Wang, Wei Wang, Xuewen Han, Guomin Cheng, Beijiu Int J Mol Sci Article Maize can form symbiotic relationships with arbuscular mycorrhiza (AM) fungus to increase productivity and resistance, but the miRNAs in maize responsible for this process have not been discovered. In this study, 155 known and 28 novel miRNAs were identified by performing high-throughput sequencing of sRNA in maize roots colonized by AM fungi. Similar to the profiles in other AM-capable plants, a large proportion of identified maize miRNAs were 24 nt in length. Fourteen and two miRNAs were significantly down- and up-regulated in response to AM fungus Glomus intraradices inoculation, respectively, suggesting potential roles of these miRNAs in AM symbiosis. Interestingly, 12 of 14 significantly down-regulated known maize miRNAs belong to the miR399 family, which was previously reported to be involved in the interaction between Medicago truncatula and AM fungi. This result indicated that the miR399 family should regulate AM symbiosis conservatively across different plant lineages. Pathway and network analyses showed that the differentially expressed miRNAs might regulate lipid metabolism and phosphate starvation response in maize during the symbiosis process via their target genes. Several members of the miR399 family and the miR397 family should be involved in controlling the fatty acid metabolism and promoting lipid delivering from plants to AM fungi. To the best of our knowledge, this is the first report on miRNAs mediating fatty acids from plant to AM fungi. This study provides insight into the regulatory roles of miRNAs in the symbiosis between plants and AM fungi. MDPI 2018-10-16 /pmc/articles/PMC6214007/ /pubmed/30332850 http://dx.doi.org/10.3390/ijms19103201 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Xu, Yunjian Zhu, Suwen Liu, Fang Wang, Wei Wang, Xuewen Han, Guomin Cheng, Beijiu Identification of Arbuscular Mycorrhiza Fungi Responsive microRNAs and Their Regulatory Network in Maize |
title | Identification of Arbuscular Mycorrhiza Fungi Responsive microRNAs and Their Regulatory Network in Maize |
title_full | Identification of Arbuscular Mycorrhiza Fungi Responsive microRNAs and Their Regulatory Network in Maize |
title_fullStr | Identification of Arbuscular Mycorrhiza Fungi Responsive microRNAs and Their Regulatory Network in Maize |
title_full_unstemmed | Identification of Arbuscular Mycorrhiza Fungi Responsive microRNAs and Their Regulatory Network in Maize |
title_short | Identification of Arbuscular Mycorrhiza Fungi Responsive microRNAs and Their Regulatory Network in Maize |
title_sort | identification of arbuscular mycorrhiza fungi responsive micrornas and their regulatory network in maize |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6214007/ https://www.ncbi.nlm.nih.gov/pubmed/30332850 http://dx.doi.org/10.3390/ijms19103201 |
work_keys_str_mv | AT xuyunjian identificationofarbuscularmycorrhizafungiresponsivemicrornasandtheirregulatorynetworkinmaize AT zhusuwen identificationofarbuscularmycorrhizafungiresponsivemicrornasandtheirregulatorynetworkinmaize AT liufang identificationofarbuscularmycorrhizafungiresponsivemicrornasandtheirregulatorynetworkinmaize AT wangwei identificationofarbuscularmycorrhizafungiresponsivemicrornasandtheirregulatorynetworkinmaize AT wangxuewen identificationofarbuscularmycorrhizafungiresponsivemicrornasandtheirregulatorynetworkinmaize AT hanguomin identificationofarbuscularmycorrhizafungiresponsivemicrornasandtheirregulatorynetworkinmaize AT chengbeijiu identificationofarbuscularmycorrhizafungiresponsivemicrornasandtheirregulatorynetworkinmaize |