Cargando…

How to Process Sputum Samples and Extract Bacterial DNA for Microbiota Analysis

Different steps and conditions for DNA extraction for microbiota analysis in sputum have been reported in the literature. We aimed at testing both dithiothreitol (DTT) and enzymatic treatments of sputum samples and identifying the most suitable DNA extraction technique for the microbiota analysis of...

Descripción completa

Detalles Bibliográficos
Autores principales:	Terranova, Leonardo, Oriano, Martina, Teri, Antonio, Ruggiero, Luca, Tafuro, Camilla, Marchisio, Paola, Gramegna, Andrea, Contarini, Martina, Franceschi, Elisa, Sottotetti, Samantha, Cariani, Lisa, Bevivino, Annamaria, Chalmers, James D., Aliberti, Stefano, Blasi, Francesco
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2018
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6214103/ https://www.ncbi.nlm.nih.gov/pubmed/30347804 http://dx.doi.org/10.3390/ijms19103256

Ejemplares similares

Comparison of different conditions for DNA extraction in sputum - a pilot study
por: Oriano, Martina, et al.
Publicado: (2019)

Pro-resolving and pro-inflammatory fatty acid-derived mediators in sputum of stable state bronchiectasis patients
por: Terranova, Leonardo, et al.
Publicado: (2022)

When and how ruling out cystic fibrosis in adult patients with bronchiectasis
por: Gramegna, Andrea, et al.
Publicado: (2018)

From Ivacaftor to Triple Combination: A Systematic Review of Efficacy and Safety of CFTR Modulators in People with Cystic Fibrosis
por: Gramegna, Andrea, et al.
Publicado: (2020)

The biology of pulmonary exacerbations in bronchiectasis
por: Amati, Francesco, et al.
Publicado: (2019)

The Isoform GC1f of the Vitamin D Binding Protein Is Associated with Bronchiectasis Severity
por: Oriano, Martina, et al.
Publicado: (2021)

Protease–Antiprotease Imbalance in Bronchiectasis
por: Oriano, Martina, et al.
Publicado: (2021)

Comparison of different sets of immunological tests to identify treatable immunodeficiencies in adult bronchiectasis patients
por: Aliberti, Stefano, et al.
Publicado: (2022)

Genetic and Serum Screening for Alpha-1-Antitrypsin Deficiency in Adult Patients with Cystic Fibrosis: A Single-Center Experience
por: Amati, Francesco, et al.
Publicado: (2022)

Why, when and how to investigate primary ciliary dyskinesia in adult patients with bronchiectasis
por: Contarini, Martina, et al.
Publicado: (2018)

Heterogeneity of weight gain after initiation of Elexacaftor/Tezacaftor/Ivacaftor in people with cystic fibrosis
por: Gramegna, Andrea, et al.
Publicado: (2023)

SARS-CoV-2 antibodies among people with cystic fibrosis prior to the vaccination campaign: A seroprevalence study in two specialized centres in Northern Italy
por: Alicandro, Gianfranco, et al.
Publicado: (2022)

Safety of mRNA-based vaccines against SARS-CoV-2 in people with cystic fibrosis aged 12 years and over
por: Alicandro, Gianfranco, et al.
Publicado: (2022)

In vitro activity of N-acetylcysteine against Stenotrophomonas maltophilia and Burkholderia cepacia complex grown in planktonic phase and biofilm
por: Pollini, Simona, et al.
Publicado: (2018)

T2-High Endotype and Response to Biological Treatments in Patients with Bronchiectasis
por: Oriano, Martina, et al.
Publicado: (2021)

Neutrophil elastase in bronchiectasis
por: Gramegna, Andrea, et al.
Publicado: (2017)

Bronchiectasis: a case-based approach to investigation and management
por: Contarini, Martina, et al.
Publicado: (2018)

Association of sputum microbiota profiles with severity of community-acquired pneumonia in children
por: Pettigrew, Melinda M., et al.
Publicado: (2016)

Azithromycin and the microbiota of cystic fibrosis sputum
por: Acosta, Nicole, et al.
Publicado: (2021)

Prevalence and incidence of bronchiectasis in Italy
por: Aliberti, Stefano, et al.
Publicado: (2020)

Environmental Arsenic Exposure and Microbiota in Induced Sputum
por: White, Allison G., et al.
Publicado: (2014)

Multicenter analysis of sputum microbiota in tuberculosis patients
por: Sala, Claudia, et al.
Publicado: (2020)

TACKLING ANTIMICROBIAL RESISTANCE (AMR) - IN VITRO EFFECT OF SODIUM CHLORIDE ON ANTIBIOTIC SUSCEPTIBILITY IN CLINICAL PSEUDOMONAS AERUGINOSA ISOLATED FROM PATIENTS WITH CYSTIC FIBROSIS (CF)
por: Gramegna, Andrea, et al.
Publicado: (2020)

The Italian registry of pulmonary non-tuberculous mycobacteria - IRENE: the study protocol
por: Aliberti, Stefano, et al.
Publicado: (2018)

Evolving serodiagnostics by rationally designed peptide arrays: the Burkholderia paradigm in Cystic Fibrosis
por: Peri, Claudio, et al.
Publicado: (2016)

Alpha-1 antitrypsin deficiency as a common treatable mechanism in chronic respiratory disorders and for conditions different from pulmonary emphysema? A commentary on the new European Respiratory Society statement
por: Gramegna, Andrea, et al.
Publicado: (2018)

SARS-CoV-2 vaccines: A critical perspective through efficacy data and barriers to herd immunity
por: Blasi, Francesco, et al.
Publicado: (2021)

Efficacy of Pirfenidone and Nintedanib in Interstitial Lung Diseases Other than Idiopathic Pulmonary Fibrosis: A Systematic Review
por: Amati, Francesco, et al.
Publicado: (2023)

In Vitro Synergism of Colistin and N-acetylcysteine against Stenotrophomonas maltophilia
por: Ciacci, Nagaia, et al.
Publicado: (2019)

Reliable and Sensitive Nested PCR for the Detection of Chlamydia in Sputum
por: Smolejová, Martina, et al.
Publicado: (2021)

Belowground Microbiota and the Health of Tree Crops
por: Mercado-Blanco, Jesús, et al.
Publicado: (2018)

Humoral and cell-mediated immune responses to BNT162b2 vaccine against SARS-CoV-2 in people with cystic fibrosis
por: Alicandro, G., et al.
Publicado: (2023)

The Impact of Soil-Applied Biochars From Different Vegetal Feedstocks on Durum Wheat Plant Performance and Rhizospheric Bacterial Microbiota in Low Metal-Contaminated Soil
por: Latini, Arianna, et al.
Publicado: (2019)

Sputum Microbiota in Tuberculosis as Revealed by 16S rRNA Pyrosequencing
por: Cheung, Man Kit, et al.
Publicado: (2013)

Sputum Microbiota Associated with New, Recurrent and Treatment Failure Tuberculosis
por: Wu, Jing, et al.
Publicado: (2013)

Lung and Gut Microbiota Changes Associated with Pseudomonas aeruginosa Infection in Mouse Models of Cystic Fibrosis
por: Bacci, Giovanni, et al.
Publicado: (2021)

Efficacy and effectiveness of Ceftaroline Fosamil in patients with pneumonia: a systematic review and meta-analysis
por: Sotgiu, Giovanni, et al.
Publicado: (2018)

Symptom-related sputum microbiota in stable chronic obstructive pulmonary disease
por: Diao, Wenqi, et al.
Publicado: (2018)

Comparing microbiota profiles in induced and spontaneous sputum samples in COPD patients
por: Tangedal, Solveig, et al.
Publicado: (2017)

Sputum microbiota as a potential diagnostic marker for multidrug-resistant tuberculosis
por: Lin, Dongzi, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_olhpqbu2qo1fi4dilnp841su20