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How to Process Sputum Samples and Extract Bacterial DNA for Microbiota Analysis

Different steps and conditions for DNA extraction for microbiota analysis in sputum have been reported in the literature. We aimed at testing both dithiothreitol (DTT) and enzymatic treatments of sputum samples and identifying the most suitable DNA extraction technique for the microbiota analysis of...

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Detalles Bibliográficos
Autores principales:	Terranova, Leonardo, Oriano, Martina, Teri, Antonio, Ruggiero, Luca, Tafuro, Camilla, Marchisio, Paola, Gramegna, Andrea, Contarini, Martina, Franceschi, Elisa, Sottotetti, Samantha, Cariani, Lisa, Bevivino, Annamaria, Chalmers, James D., Aliberti, Stefano, Blasi, Francesco
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2018
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6214103/ https://www.ncbi.nlm.nih.gov/pubmed/30347804 http://dx.doi.org/10.3390/ijms19103256

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