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Synthesis of Indoleacetic Acid, Gibberellic Acid and ACC-Deaminase by Mortierella Strains Promote Winter Wheat Seedlings Growth under Different Conditions

The endogenous pool of phytoregulators in plant tissues supplied with microbial secondary metabolites may be crucial for the development of winter wheat seedlings during cool springs. The phytohormones may be synthesized by psychrotrophic microorganisms in lower temperatures occurring in a temperate...

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Autores principales: Ozimek, Ewa, Jaroszuk-Ściseł, Jolanta, Bohacz, Justyna, Korniłłowicz-Kowalska, Teresa, Tyśkiewicz, Renata, Słomka, Anna, Nowak, Artur, Hanaka, Agnieszka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6214141/
https://www.ncbi.nlm.nih.gov/pubmed/30340353
http://dx.doi.org/10.3390/ijms19103218
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author Ozimek, Ewa
Jaroszuk-Ściseł, Jolanta
Bohacz, Justyna
Korniłłowicz-Kowalska, Teresa
Tyśkiewicz, Renata
Słomka, Anna
Nowak, Artur
Hanaka, Agnieszka
author_facet Ozimek, Ewa
Jaroszuk-Ściseł, Jolanta
Bohacz, Justyna
Korniłłowicz-Kowalska, Teresa
Tyśkiewicz, Renata
Słomka, Anna
Nowak, Artur
Hanaka, Agnieszka
author_sort Ozimek, Ewa
collection PubMed
description The endogenous pool of phytoregulators in plant tissues supplied with microbial secondary metabolites may be crucial for the development of winter wheat seedlings during cool springs. The phytohormones may be synthesized by psychrotrophic microorganisms in lower temperatures occurring in a temperate climate. Two fungal isolates from the Spitzbergen soils after the microscopic observations and “the internal transcribed spacer” (ITS) region molecular characterization were identified as Mortierella antarctica (MA DEM7) and Mortierella verticillata (MV DEM32). In order to study the synthesis of indoleacetic acid (IAA) and gibberellic acid (GA), Mortierella strains were grown on media supplemented with precursor of phytohormones tryptophan at 9, 15 °C, and 20 °C for nine days. The highest amount of IAA synthesis was identified in MV DEM32 nine-day-culture at 15 °C with 1.5 mM of tryptophan. At the same temperature (15 °C), the significant promoting effect (about 40% root and shoot fresh weight) of this strain on seedlings was observed. However, only MA DEM-7 had the ACC (1-aminocyclopropane-1-carboxylate) deaminase activity with the highest efficiency at 9 °C and synthesized IAA without tryptophan. Moreover, at the same conditions, the strain was confirmed to possess the strong promoting effect (about 40% root and 24% shoot fresh weight) on seedlings. Both strains synthesized GA in all tested terms and temperatures. The studied Mortierella strains had some important traits that led them to be considered as microbial biofertilizers components, improving plant growth in difficult temperate climates.
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spelling pubmed-62141412018-11-14 Synthesis of Indoleacetic Acid, Gibberellic Acid and ACC-Deaminase by Mortierella Strains Promote Winter Wheat Seedlings Growth under Different Conditions Ozimek, Ewa Jaroszuk-Ściseł, Jolanta Bohacz, Justyna Korniłłowicz-Kowalska, Teresa Tyśkiewicz, Renata Słomka, Anna Nowak, Artur Hanaka, Agnieszka Int J Mol Sci Article The endogenous pool of phytoregulators in plant tissues supplied with microbial secondary metabolites may be crucial for the development of winter wheat seedlings during cool springs. The phytohormones may be synthesized by psychrotrophic microorganisms in lower temperatures occurring in a temperate climate. Two fungal isolates from the Spitzbergen soils after the microscopic observations and “the internal transcribed spacer” (ITS) region molecular characterization were identified as Mortierella antarctica (MA DEM7) and Mortierella verticillata (MV DEM32). In order to study the synthesis of indoleacetic acid (IAA) and gibberellic acid (GA), Mortierella strains were grown on media supplemented with precursor of phytohormones tryptophan at 9, 15 °C, and 20 °C for nine days. The highest amount of IAA synthesis was identified in MV DEM32 nine-day-culture at 15 °C with 1.5 mM of tryptophan. At the same temperature (15 °C), the significant promoting effect (about 40% root and shoot fresh weight) of this strain on seedlings was observed. However, only MA DEM-7 had the ACC (1-aminocyclopropane-1-carboxylate) deaminase activity with the highest efficiency at 9 °C and synthesized IAA without tryptophan. Moreover, at the same conditions, the strain was confirmed to possess the strong promoting effect (about 40% root and 24% shoot fresh weight) on seedlings. Both strains synthesized GA in all tested terms and temperatures. The studied Mortierella strains had some important traits that led them to be considered as microbial biofertilizers components, improving plant growth in difficult temperate climates. MDPI 2018-10-18 /pmc/articles/PMC6214141/ /pubmed/30340353 http://dx.doi.org/10.3390/ijms19103218 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ozimek, Ewa
Jaroszuk-Ściseł, Jolanta
Bohacz, Justyna
Korniłłowicz-Kowalska, Teresa
Tyśkiewicz, Renata
Słomka, Anna
Nowak, Artur
Hanaka, Agnieszka
Synthesis of Indoleacetic Acid, Gibberellic Acid and ACC-Deaminase by Mortierella Strains Promote Winter Wheat Seedlings Growth under Different Conditions
title Synthesis of Indoleacetic Acid, Gibberellic Acid and ACC-Deaminase by Mortierella Strains Promote Winter Wheat Seedlings Growth under Different Conditions
title_full Synthesis of Indoleacetic Acid, Gibberellic Acid and ACC-Deaminase by Mortierella Strains Promote Winter Wheat Seedlings Growth under Different Conditions
title_fullStr Synthesis of Indoleacetic Acid, Gibberellic Acid and ACC-Deaminase by Mortierella Strains Promote Winter Wheat Seedlings Growth under Different Conditions
title_full_unstemmed Synthesis of Indoleacetic Acid, Gibberellic Acid and ACC-Deaminase by Mortierella Strains Promote Winter Wheat Seedlings Growth under Different Conditions
title_short Synthesis of Indoleacetic Acid, Gibberellic Acid and ACC-Deaminase by Mortierella Strains Promote Winter Wheat Seedlings Growth under Different Conditions
title_sort synthesis of indoleacetic acid, gibberellic acid and acc-deaminase by mortierella strains promote winter wheat seedlings growth under different conditions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6214141/
https://www.ncbi.nlm.nih.gov/pubmed/30340353
http://dx.doi.org/10.3390/ijms19103218
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