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Effects of OsCDPK1 on the Structure and Physicochemical Properties of Starch in Developing Rice Seeds

Overexpression of a constitutively active truncated form of OsCDPK1 (OEtr) in rice produced smaller seeds, but a double-stranded RNA gene-silenced form of OsCDPK1 (Ri) yielded larger seeds, suggesting that OsCDPK1 plays a functional role in rice seed development. In the study presented here, we prop...

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Autores principales: Jiang, Jian-Zhi, Kuo, Chun-Hsiang, Chen, Bo-Hong, Chen, Mao-Kei, Lin, Choun-Sea, Ho, Shin-Lon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6214144/
https://www.ncbi.nlm.nih.gov/pubmed/30347730
http://dx.doi.org/10.3390/ijms19103247
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author Jiang, Jian-Zhi
Kuo, Chun-Hsiang
Chen, Bo-Hong
Chen, Mao-Kei
Lin, Choun-Sea
Ho, Shin-Lon
author_facet Jiang, Jian-Zhi
Kuo, Chun-Hsiang
Chen, Bo-Hong
Chen, Mao-Kei
Lin, Choun-Sea
Ho, Shin-Lon
author_sort Jiang, Jian-Zhi
collection PubMed
description Overexpression of a constitutively active truncated form of OsCDPK1 (OEtr) in rice produced smaller seeds, but a double-stranded RNA gene-silenced form of OsCDPK1 (Ri) yielded larger seeds, suggesting that OsCDPK1 plays a functional role in rice seed development. In the study presented here, we propose a model in which OsCDPK1 plays key roles in negatively controlling the grain size, amylose content, and endosperm appearance, and also affects the physicochemical properties of the starch. The dehulled transgenic OEtr grains were smaller than the dehulled wild-type grains, and the OEtr endosperm was opaque and had a low amylose content and numerous small loosely packed polyhedral starch granules. However, the OEtr grain sizes and endosperm appearances were not affected by temperature, which ranged from low (22 °C) to high (31 °C) during the grain-filling phase. In contrast, the transgenic Ri grains were larger, had higher amylose content, and had more transparent endosperms filled with tightly packed polyhedral starch granules. This demonstrates that OsCDPK1 plays a novel functional role in starch biosynthesis during seed development and affects the transparent appearance of the endosperm. These results improve our understanding of the molecular mechanisms through which the grain-filling process occurs in rice.
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spelling pubmed-62141442018-11-14 Effects of OsCDPK1 on the Structure and Physicochemical Properties of Starch in Developing Rice Seeds Jiang, Jian-Zhi Kuo, Chun-Hsiang Chen, Bo-Hong Chen, Mao-Kei Lin, Choun-Sea Ho, Shin-Lon Int J Mol Sci Article Overexpression of a constitutively active truncated form of OsCDPK1 (OEtr) in rice produced smaller seeds, but a double-stranded RNA gene-silenced form of OsCDPK1 (Ri) yielded larger seeds, suggesting that OsCDPK1 plays a functional role in rice seed development. In the study presented here, we propose a model in which OsCDPK1 plays key roles in negatively controlling the grain size, amylose content, and endosperm appearance, and also affects the physicochemical properties of the starch. The dehulled transgenic OEtr grains were smaller than the dehulled wild-type grains, and the OEtr endosperm was opaque and had a low amylose content and numerous small loosely packed polyhedral starch granules. However, the OEtr grain sizes and endosperm appearances were not affected by temperature, which ranged from low (22 °C) to high (31 °C) during the grain-filling phase. In contrast, the transgenic Ri grains were larger, had higher amylose content, and had more transparent endosperms filled with tightly packed polyhedral starch granules. This demonstrates that OsCDPK1 plays a novel functional role in starch biosynthesis during seed development and affects the transparent appearance of the endosperm. These results improve our understanding of the molecular mechanisms through which the grain-filling process occurs in rice. MDPI 2018-10-19 /pmc/articles/PMC6214144/ /pubmed/30347730 http://dx.doi.org/10.3390/ijms19103247 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jiang, Jian-Zhi
Kuo, Chun-Hsiang
Chen, Bo-Hong
Chen, Mao-Kei
Lin, Choun-Sea
Ho, Shin-Lon
Effects of OsCDPK1 on the Structure and Physicochemical Properties of Starch in Developing Rice Seeds
title Effects of OsCDPK1 on the Structure and Physicochemical Properties of Starch in Developing Rice Seeds
title_full Effects of OsCDPK1 on the Structure and Physicochemical Properties of Starch in Developing Rice Seeds
title_fullStr Effects of OsCDPK1 on the Structure and Physicochemical Properties of Starch in Developing Rice Seeds
title_full_unstemmed Effects of OsCDPK1 on the Structure and Physicochemical Properties of Starch in Developing Rice Seeds
title_short Effects of OsCDPK1 on the Structure and Physicochemical Properties of Starch in Developing Rice Seeds
title_sort effects of oscdpk1 on the structure and physicochemical properties of starch in developing rice seeds
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6214144/
https://www.ncbi.nlm.nih.gov/pubmed/30347730
http://dx.doi.org/10.3390/ijms19103247
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